siRNA-loaded nanoparticles for CT/fluorescence dual-modality imaging and preparation methods and applications
A dual-mode imaging and CT imaging technology is applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, and preparations for in vivo tests, etc. It can solve the problem of providing comprehensive information and tissue penetration capabilities. Low-level problems, to achieve good blood pool contrast effect, long blood circulation time, and prolong the effect of CT imaging time window
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[0037] The present invention will be described in detail below in conjunction with the accompanying drawings and test examples.
[0038] Preparation of nanoparticles of the present invention
[0039] 1) Prepare the solution
[0040] ⅰ) Au-siRNA aqueous phase solution
[0041]First prepare gold nanoparticles: soak the experimental apparatus with aqua regia overnight, wash fully with deionized water, add 1 volume of 1% chloroauric acid solution to 80 volumes of water, stir magnetically for 5 minutes, and then add 7 volumes of gold nanoparticles at one time. 1% sodium citrate solution, after stirring for 5 minutes, slowly add 8 volumes of newly prepared 0.05% sodium borohydride at 4°C dropwise, the solution gradually turns wine red, and continue stirring for 30 minutes to obtain gold nanoparticles. Add 1% pure diethylpyrocarbonate (DEPC) solution by volume and stir overnight, then sterilize under high temperature and high pressure (120°C, 15min) to remove the ribozyme that can ...
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