siRNA-loaded nanoparticles for CT/fluorescence dual-modality imaging and preparation methods and applications

A dual-mode imaging and CT imaging technology is applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, and preparations for in vivo tests, etc. It can solve the problem of providing comprehensive information and tissue penetration capabilities. Low-level problems, to achieve good blood pool contrast effect, long blood circulation time, and prolong the effect of CT imaging time window

Active Publication Date: 2021-06-25
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Fluorescence imaging generally has high sensitivity, and low concentration fluorescent probes can perform obvious fluorescence imaging, but its application is limited by low tissue penetration ability
Any imaging mode has its inherent defects, and it is difficult to provide comprehensive information by only one imaging mode

Method used

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  • siRNA-loaded nanoparticles for CT/fluorescence dual-modality imaging and preparation methods and applications
  • siRNA-loaded nanoparticles for CT/fluorescence dual-modality imaging and preparation methods and applications
  • siRNA-loaded nanoparticles for CT/fluorescence dual-modality imaging and preparation methods and applications

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Embodiment Construction

[0037] The present invention will be described in detail below in conjunction with the accompanying drawings and test examples.

[0038] Preparation of nanoparticles of the present invention

[0039] 1) Prepare the solution

[0040] ⅰ) Au-siRNA aqueous phase solution

[0041]First prepare gold nanoparticles: soak the experimental apparatus with aqua regia overnight, wash fully with deionized water, add 1 volume of 1% chloroauric acid solution to 80 volumes of water, stir magnetically for 5 minutes, and then add 7 volumes of gold nanoparticles at one time. 1% sodium citrate solution, after stirring for 5 minutes, slowly add 8 volumes of newly prepared 0.05% sodium borohydride at 4°C dropwise, the solution gradually turns wine red, and continue stirring for 30 minutes to obtain gold nanoparticles. Add 1% pure diethylpyrocarbonate (DEPC) solution by volume and stir overnight, then sterilize under high temperature and high pressure (120°C, 15min) to remove the ribozyme that can ...

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Abstract

The invention discloses a nanoparticle loaded with siRNA for CT / fluorescence dual-mode imaging, a preparation method and application thereof. The nanoparticle comprises a carrier component, a drug component, a CT imaging component and a fluorescent imaging component. The carrier component is lipid, the drug component is siRNA, the CT imaging component is gold nanoparticles with a particle size of about 5nm, and the fluorescent imaging component is Cy5 (carbon cyanide dye). Nanoparticles formed by encapsulating siRNA, gold nanoparticles and Cy5 with lipid molecules are prepared by ethanol dilution method, which can effectively overcome the delivery barrier of drug component siRNA in vivo, and can also endow gold nanoparticles with tumor targeting properties that they do not have. and longer CT imaging time windows.

Description

technical field [0001] The invention relates to the technical field of multimodal imaging and siRNA delivery, in particular to a nanoparticle loaded with siRNA for CT / fluorescence dual-modal imaging and its preparation method and application, which is to simultaneously realize CT imaging and fluorescence imaging to monitor the release position of siRNA in vivo Efficient method for point and time. Background technique [0002] siRNA has attracted much attention because of its remarkable gene silencing effect. However, naked siRNA has the disadvantages of being easily degraded by ribozymes, poor endocytosis and poor cell targeting ability, so it is particularly critical to establish a suitable gene delivery system. Nano-delivery systems have been extensively studied for their safety, low toxicity, low immunogenicity, targeting, and ease of assembly. Hydrophilic polymers such as polyethylene glycol (PEG) modified on the surface of nanoparticles can increase the circulation ti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K49/04A61K31/7105A61K9/51A61K47/44A61K47/28A61P35/00
CPCA61K9/5123A61K31/7105A61K49/0002A61K49/0021A61K49/04A61P35/00
Inventor 俞磊张金芳沈鸿伟谭婧雯刘丽王镜闫志强
Owner EAST CHINA NORMAL UNIV
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