MSRV (micropterus salmoides rhabdovirus) egg yolk antibody

A technology of yolk antibody and largemouth bass, which is applied in the direction of antibodies, antiviral agents, antiviral immunoglobulin, etc., can solve the problems of immature immune system of fish fry and the difficulty of active immune prevention, etc., so as to terminate virus infection and obtain Convenience, high-yield results

Active Publication Date: 2019-03-08
ZHEJIANG INST OF FRESH WATER FISHERIES
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Problems solved by technology

Since the rhabdovirus mainly harms perch fry below 5cm, and the immune system of f...

Method used

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  • MSRV (micropterus salmoides rhabdovirus) egg yolk antibody
  • MSRV (micropterus salmoides rhabdovirus) egg yolk antibody
  • MSRV (micropterus salmoides rhabdovirus) egg yolk antibody

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Embodiment

[0029] A largemouth bass rhabdovirus yolk antibody prepared by the following method:

[0030] 1. Isolation and culture of rhabdovirus from largemouth bass

[0031] Get the largemouth bass fry (from the breeding factory in Huzhou City, Zhejiang Province, body length 2-5cm) infected with MSRV, freeze and thaw twice after homogenization, centrifuge 30min at 12000rpm, get the supernatant and filter through a 0.25 μm bacterial filter, The filtrate was the crude virus extract, which was aliquoted and stored at -80°C for future use.

[0032] Grass carp ovary cell line CO cells were used as sensitive cells for largemouth bass rhabdovirus. The culture medium for CO cells was M199 complete medium (10% fetal bovine serum, 100 μg / mL penicillin, and 100 μg / mL streptomycin were added to M199 basal medium). Cultivate in a cell culture flask of T25 specification, add 4-5 milliliters of M199 complete medium, when the CO cells are at a confluence of 60-80%, suck out the culture solution, add ...

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Abstract

The invention discloses an MSRV (micropterus salmoides rhabdovirus) egg yolk antibody. The antibody is prepared with the following method: (1) MSRV is obtained from micropterus salmoides infected withthe MSRV through separation and culture; (2) the MSRV is inactivated; (3) laying hens of 180-190 day ages are immunized with the inactivated MSRV as an antigen, eggs of immunized laying hens are collected, and the egg yolk antibody is extracted and purified from egg yolk of the eggs. The antibody neutralizes the MSRV effectively to terminate virus infection, provides an effective passive immunitypreparation for prevention and control of the virus later and has great significance in decreasing virus carrying rate of parents and increasing survival rate of chicks.

Description

technical field [0001] The invention relates to the technical field of production of fish biological products, in particular to a largemouth bass rhabdovirus yolk antibody. Background technique [0002] Largemouth bass Micropterus salmoides, commonly known as California perch, is native to the Mississippi River system in California, USA. It has delicious meat and is very popular in the market. In addition, it has the characteristics of rapid growth, easy to catch, wide temperature range, and strong low oxygen resistance. Very popular with farmers. my country's annual output can reach more than 300,000 tons, which is the country with the highest output in the world. In 2016, the breeding area of ​​largemouth bass in Zhejiang Province was nearly 50,000 mu. In recent years, California perch has become the main species of raceway recirculating aquaculture. However, whether it is traditional high-density breeding or track-style centralized captivity, there is a potential dange...

Claims

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Application Information

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IPC IPC(8): C07K16/10C07K16/02A61K39/42A61P31/14
CPCA61K2039/505A61P31/14C07K16/10C07K2317/11
Inventor 刘莉吕孙建袁雪梅施伟达杭小英吴颖蕾
Owner ZHEJIANG INST OF FRESH WATER FISHERIES
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