Transparent agent for treating root tip of plant and application thereof

Abstract

The invention provides a transparent agent for treating a root tip of a plant, comprising hydrated trichloroacetaldehyde, glycerin and water. The ratio of the hydrated trichloroacetaldehyde, the glycerin to the water in g/ml/ml is 3-9:1:3, wherein the pH of the clearing agent is 1.1-7.5. The invention also provides the application of the transparent agent in transparent treatment of the root tip of the plant, a transparent treatment method and a method for adjusting transparent treatment conditions of the root tip of the plant. The transparent agent provided by the invention adjusts the groupdistribution ratio in the existing transparent agent, and is applied to a material for treating the root tip of the plant to obtain the root tip of the plant with small degree of cell plasmolysis, normal shape, high definition and suitable observation. The transparent agent is beneficial to observe and statistically and quantitatively analyze the root tip of the plant. The optimal conditions for the transparent treatment of the root tip of the plant can also be filtered according to the transparent treatment effect of the root tip, the proportion of cell plasmolysis is reduced, the proportionof normal morphological cells is increased, the observation definition of cells is improved, and reference is provided for the development of the overall transparency technology of the root tip of theplant.

Description

technical field [0001] The application relates to the field of plant microscopy technology, in particular to a transparent agent for treating plant root tips and its application. Background technique [0002] The overall transparent observation technology is one of the basic means of plant science research, and it is a convenient method to directly observe the internal structure of plants without sectioning. This technology uses high-refractive-index media to increase the luminous flux, increase the depth of field, and allow more images on the focal plane to enter the observation field, so that the tissue sample is transparent, and due to the difference in the thickness and refractive index of different tissues, it presents different shapes. . The transparent tissue can be used without staining, and the tissues and cells that are usually not easy to be observed can be observed by bright field microscope, phase contrast microscope, differential interference contrast microsco...

AI Technical Summary

Problems solved by technology

However, for plant root tips, since the cell walls of root tip cells are relatively thin, they are more sensitive to chemical reagent treatment. Using existing clearing agents and clearing treatment methods to treat plant root tips will lead to a large proportion of root tip cells. Separation of the plasmodium leads to swelling or curling of the cell morphology, and the clarity of observation is poor. Therefore, it is difficult to obtain an overall transparent root tip with ideal shape, which is not conducive to the observation, statistics or measurement analysis of root tip cells.

However, in the prior art, no clearing agent, clearing treatment method, and method for optimizing root tip clearing conditions that can maintain the integrity of plant root tip cell morphology and high clarity of observation have not been proposed.

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