A kind of active microalgae skin care liquid and its preparation method and application
A technology of skin care lotion and microalgae solution, which is applied in the field of skin care products, can solve the problems of limited skin care nutrients, limit the efficacy of emulsions, and burden, and achieve rich skin care nutrients, enhanced skin care efficacy, good compatibility and symbiosis Effect
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Embodiment 1
[0036] A kind of active microalgae skin care liquid, the preparation method comprises the following steps:
[0037] S1, expanding and cultivating Chlorella pyrenoidosa and Spirulina respectively to obtain three kinds of algae liquids, wherein, in the process of expanding cultivation,
[0038] The expanded cultivation process of the two algae liquids includes: selecting and separating a single microalgal cell under a microscope → culturing a single microalgal cell in 15 mL of medium solution for 10 days to obtain 15 mL of algal liquid → transfer to 85 mL of medium solution Continue culturing for 10 days to obtain 100mL of algae solution → inoculate into 0.9L medium solution for 10 days to obtain 1L of algae solution → inoculate into 4L medium solution for 10 days to obtain 5L Algae liquid → inoculated into 13L medium solution and continued to cultivate for 10 days to obtain 18L of algae liquid → transferred to 782L medium solution and continued to cultivate for 10 days to obtai...
Embodiment 2
[0043] The difference with embodiment 1 is:
[0044] In the expanded cultivation of step S1,
[0045] The expanded cultivation process of the two algae solutions includes: selecting and separating a single microalgae cell under a microscope → culturing a single microalgae cell in 15mL of medium solution for 12 days to obtain 15mL of algae solution → transfer to 85mL of medium solution Continue culturing for 10 days to obtain 100mL of algae liquid → inoculate into 0.9L medium solution for 12 days to obtain 1L of algae liquid → inoculate into 4L medium solution for 12 days to obtain 5L Algae liquid → inoculated into 13L medium solution and continued to cultivate for 12 days to obtain 18L of algae liquid → transferred to 782L medium solution and continued to cultivate for 12 days to obtain 800L of algae liquid. At the end of the expansion of each stage above, the number of viable microalgae cells is ≥10 6 cells / mL, the number of active microalgae cells in the final obtained alg...
Embodiment 3
[0048] The difference with embodiment 1 is:
[0049] In the expanded cultivation of step S1,
[0050] The expanded cultivation process of the two algal liquids includes: selecting and separating a single microalgal cell under a microscope → culturing a single microalgal cell in 15 mL of medium solution for 15 days to obtain 15 mL of algal liquid → transfer to 85 mL of medium solution Continue culturing for 15 days, obtain 100mL of algae liquid → inoculate into 0.9L medium solution and continue culturing for 15 days, obtain 1L of algae liquid → transfer and inoculate into 4L medium solution for 15 days, obtain 5L Algae liquid → inoculated into 13L medium solution and continued to cultivate for 15 days to obtain 18L of algae liquid → transferred to 782L medium solution and continued to cultivate for 15 days to obtain 800L of algae liquid. At the end of the expansion of each stage above, the number of viable microalgae cells is ≥10 6 cells / mL, the number of active microalgae ce...
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