A method for automatically extracting target cell mitochondria in vitro

Abstract

The invention discloses a method for automatically extracting target cell mitochondria, which belongs to a micro-nano robot auxiliary control system, and realizes automatic cell pose control and two-dimensional cell biopsy operation. The invention includes: collecting cell images through a CCD camera connected to a fluorescence microscope, identifying the position and attitude angle of the target mitochondria to be extracted through an image processing algorithm; and realizing precise control of the cell position and attitude angle through optical tweezers in different operating modes; Negative pressure is applied through the external pump of the microfluidic chip, so that the target cells are firmly fixed in the channel of the microfluidic chip to keep the posture unchanged; the tip of the glass microtube is moved to the vicinity of the target mitochondria to be extracted by using the visual feedback control strategy; The negative pressure applied by the pump connected to the microtube will extract the mitochondria to be extracted out of the target cells.

Description

technical field [0001] The invention belongs to a micro-nano robot auxiliary control system, in particular to a method for automatically extracting mitochondria of target cells in vitro. Background technique [0002] Bioanalysis at the single-cell level can capture cell heterogeneity and has received extensive attention in recent years. As a single-cell biopsy technique, cell biopsy achieves disease diagnosis and treatment by extracting and analyzing tissues or organelles at the subcellular level. Therefore, it is imperative to develop highly precise micromanipulation tools capable of manipulating and extracting organelles and intracellular components to facilitate the rapid development of new single-cell-based medical therapies, thereby facilitating in-depth insights into cellular dynamics, cellular component functions, and disease mechanisms. understand. [0003] In the past decades, a variety of micro / nano manipulation tools for intracellular level manipulations have be...

AI Technical Summary

Problems solved by technology

These operating systems and tools are generally implemented by open-loop manual methods, which require high operational proficiency and require a lot of time for operational training, poor repeatability of experiments, and low operational accuracy.

Cell biopsy manipulation tasks, such as locating the position of the microtubule tip relative to the cell, locating the glass microtubule at a specific location within the cell after piercing the cell, and extracting tiny amounts of tissue from the cell, are tedious and time-consuming

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