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Biocontrol preparation with nematicidal function and preparation method thereof

A technology of biological control agents and nematicides, applied in the direction of nematicides, biocides, biocides, etc., can solve the problems of endangering human health, environmental pollution, damage, etc., and achieve the effect of balanced pH value and low environmental pollution

Active Publication Date: 2019-03-22
YUNNAN AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the method of preventing and controlling root-knot nematode is mostly chemical agent control, that is, applying compounds such as gramamifos, abamectin EC, phoxim EC, thiamethoxam water dispersible granule in the crop soil, but chemical control is harmful to the soil environment. Often cause irreparable damage, chemical insecticide methods cause environmental pollution and endanger human health
Field rotation and the use of non-host plants or disease-resistant plants are the main management measures to control root-knot nematodes, but they have certain limitations. For example, most species of root-knot nematodes have a wide host range. Most of the population is a mixed population of several species, and the control effect is difficult to sustain
Other measures such as sun exposure, flooding, use of disease-free seedlings, and soil improvement can achieve some control effects in some cases, but the effect is minimal or has relatively large limitations

Method used

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  • Biocontrol preparation with nematicidal function and preparation method thereof
  • Biocontrol preparation with nematicidal function and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 1: Preparation of a biocontrol agent with nematode function

[0027] ①Cultivation of Acremonium acremonium

[0028] Inoculate the Acremonium acremonium on the PDA plate medium at a temperature of 28°C for 5-7 days, and inoculate the Acremonium acremonium cultured on the PDA plate medium into 100mL LB liquid medium in an ultra-clean workbench In the medium, the rotation speed is 180rpm, the temperature is 28°C and the shaking culture is for three days, and the cultured bacterial liquid is inoculated into 1L of sterile LB liquid medium per 100mL, the rotation speed is 180rpm, and the temperature is 28°C shaking culture for three days;

[0029] ② Obtain the freeze-dried powder of the culture medium

[0030] Centrifuge the acremonium mold liquid obtained in step ① at a speed of 12000 rpm for 10 minutes, retain the supernatant after centrifugation, remove the precipitate, filter the supernatant with a 0.45 μm filter, and freeze-dry at low temperature until the water...

Embodiment 2

[0034] Embodiment two: research on the insecticidal effect of the culture solution of acremonium acremonium

[0035] 1 Isolation and culture of nematodes

[0036] a. Rinse the tomato roots containing root-knot nematodes, cut them as much as possible with scissors, and put them into a grinder to make them fully crushed.

[0037] b. Pour the crushed root material into the sampling sieve for sieving nematodes (the upper one is 320 mesh, the lower one is 500 mesh, and the two are stacked together) to sieve nematode eggs, and rinse them in an appropriate amount of running water The crushed root material (the sieve is tilted for flushing) for 5 minutes, the nematode ovum mass will leak into the second sieve, and then the nematode ovum in the 500 mesh sieve will be rinsed into the petri dish with a washing bottle, and the sieve will be repeated once.

[0038] c. Observe whether nematode eggs and the number of eggs are screened under the stereoscope. If there are nematode eggs, they ...

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Abstract

The invention relates to the technical field of biological pesticides, in particular to a biocontrol preparation with a nematicidal function and a preparation method of the biocontrol preparation withthe nematicidal function. The biocontrol preparation with the nematicidal function provided by the invention comprises the following main components in parts by weight: 3 to 7 parts of plant ash, 8 to 18 parts of borax, and 5 to 10 parts of lyophilized powder of an acremonium yunnanensis culture solution. The invention further provides the preparation method of the biocontrol preparation, comprising the following steps: collecting the culture solution after culturing the acremonium yunnanensis, freeze-drying and adding the plant ash, borax and sawdust. The active component in the biocontrol preparation provided by the invention is the lyophilized powder of the acremonium yunnanensis culture solution, and has a very good root-knot nematode killing effect.

Description

technical field [0001] The invention relates to the technical field of biopesticides, in particular to a biocontrol preparation with a nematode function and a preparation method thereof. Background technique [0002] Root-knot nematode (Meloidogyne) is a highly specialized omnivorous plant pathogenic nematode, dioecious. The larvae are elongated and worm-like, harmful to vegetables, and have a wide range of hosts, and can survive for one year without hosts. The upper part of the damaged plant grows short and slow, with abnormal leaf color, few results, low yield, and even premature death of the plant. Nematodes that are known to harm vegetables mainly include root-knot nematode high pedicle, root-knot nematode peanut, root-knot nematode northern and root-knot nematode southern. Nematodes have a wide range of hosts and often damage more than 30 kinds of vegetables such as melons, solanaceous fruits, beans, radishes, carrots, lettuce, and cabbage, and can also spread some fu...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N61/00A01N59/14A01P5/00
CPCA01N59/14A01N61/00A01N63/30
Inventor 杜云龙万媛媛韩丽赵宜婷周璇武丽霞张利娜彭晟何霞红
Owner YUNNAN AGRICULTURAL UNIVERSITY
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