Extraction method of clerodendranthus spicatus total flavone extract
An extraction method and technology of total flavonoids are applied in the fields of medicine, food and health care products, which can solve the problems of restricting in-depth development and application, low yield and purity of flavonoids, etc. Level down effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0033]Extraction: Take kidney tea (also known as: cat's beard grass leaves, purchased from Tongrentang Pharmaceutical Co., Ltd.) powder with a mesh number of 10, add 60% ethanol aqueous solution at a liquid-to-material ratio of 10L / Kg, and soak at room temperature for 24 hours Stir while soaking, 280W ultrasonic (ultrasonic instrument model is UC-10, purchased from Shanghai Titan Technology Co., Ltd.) treatment for 30min, the obtained extract is filtered with medium-speed quantitative filter paper (pore size 20-50μm), and the filtrate is concentrated by rotary evaporation get a paste;
[0034] Purification: The above paste was separated with AB-8 (purchased from Beijing Suo Lai Biotechnology Co., Ltd.) macroporous adsorption resin column, 3BV was eluted with water to remove impurities, 3BV was eluted with 70% ethanol, and the collection concentration was 70% ethanol eluate, recover ethanol under reduced pressure, and dry under reduced pressure to obtain total flavonoids extrac...
Embodiment 2
[0036] Extraction: Take kidney tea (also known as: cat's beard grass leaves, purchased from Tongrentang Pharmaceutical Co., Ltd.) powder with a mesh number of 10, add 70% ethanol aqueous solution at a liquid-to-material ratio of 20L / Kg, and soak at room temperature for 24 hours Stir while soaking, 280W ultrasonic (ultrasonic instrument model is UC-10, purchased from Shanghai Titan Technology Co., Ltd.) treatment for 30min, the obtained extract is filtered with medium-speed quantitative filter paper (pore size 20-50μm), and the filtrate is concentrated by rotary evaporation get a paste;
[0037] Purification: the above paste is separated by AB-8 macroporous adsorption resin column, 4BV of water is eluted to remove impurities, 4BV of ethanol with a concentration of 70% is eluted, and the ethanol eluate with a concentration of 70% is collected, and the ethanol is recovered under reduced pressure. The total flavone extract was obtained by drying under reduced pressure, the final t...
Embodiment 3
[0039] Extraction: Take kidney tea (also known as: cat's beard grass leaves, purchased from Tongrentang Pharmaceutical Co., Ltd.) powder with a mesh number of 10, add 80% ethanol aqueous solution at a liquid-to-material ratio of 25L / Kg, and soak at room temperature for 24 hours Stir while soaking, 280W ultrasonic (ultrasonic instrument model is UC-10, purchased from Shanghai Titan Technology Co., Ltd.) treatment for 30min, the obtained extract is filtered with medium-speed quantitative filter paper (pore size 20-50μm), and the filtrate is concentrated by rotary evaporation get a paste;
[0040] Purification: The above paste is separated by AB-8 macroporous adsorption resin column, 2BV of water is eluted to remove impurities, 2BV of ethanol with a concentration of 70% is eluted, and the ethanol eluate with a concentration of 70% is collected, and the ethanol is recovered under reduced pressure. The total flavonoid extract was obtained by drying under reduced pressure, and the f...
PUM
Property | Measurement | Unit |
---|---|---|
Mesh | aaaaa | aaaaa |
Mesh | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com