Extraction method of clerodendranthus spicatus total flavone extract

An extraction method and technology of total flavonoids are applied in the fields of medicine, food and health care products, which can solve the problems of restricting in-depth development and application, low yield and purity of flavonoids, etc. Level down effect

Inactive Publication Date: 2019-03-29
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the fat-soluble flavonoid aglycone compounds obtained in the patent are crude extracts, and the yield and purity of flavonoids are very low, which restricts its in-depth development and application

Method used

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  • Extraction method of clerodendranthus spicatus total flavone extract
  • Extraction method of clerodendranthus spicatus total flavone extract
  • Extraction method of clerodendranthus spicatus total flavone extract

Examples

Experimental program
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Effect test

Embodiment 1

[0033]Extraction: Take kidney tea (also known as: cat's beard grass leaves, purchased from Tongrentang Pharmaceutical Co., Ltd.) powder with a mesh number of 10, add 60% ethanol aqueous solution at a liquid-to-material ratio of 10L / Kg, and soak at room temperature for 24 hours Stir while soaking, 280W ultrasonic (ultrasonic instrument model is UC-10, purchased from Shanghai Titan Technology Co., Ltd.) treatment for 30min, the obtained extract is filtered with medium-speed quantitative filter paper (pore size 20-50μm), and the filtrate is concentrated by rotary evaporation get a paste;

[0034] Purification: The above paste was separated with AB-8 (purchased from Beijing Suo Lai Biotechnology Co., Ltd.) macroporous adsorption resin column, 3BV was eluted with water to remove impurities, 3BV was eluted with 70% ethanol, and the collection concentration was 70% ethanol eluate, recover ethanol under reduced pressure, and dry under reduced pressure to obtain total flavonoids extrac...

Embodiment 2

[0036] Extraction: Take kidney tea (also known as: cat's beard grass leaves, purchased from Tongrentang Pharmaceutical Co., Ltd.) powder with a mesh number of 10, add 70% ethanol aqueous solution at a liquid-to-material ratio of 20L / Kg, and soak at room temperature for 24 hours Stir while soaking, 280W ultrasonic (ultrasonic instrument model is UC-10, purchased from Shanghai Titan Technology Co., Ltd.) treatment for 30min, the obtained extract is filtered with medium-speed quantitative filter paper (pore size 20-50μm), and the filtrate is concentrated by rotary evaporation get a paste;

[0037] Purification: the above paste is separated by AB-8 macroporous adsorption resin column, 4BV of water is eluted to remove impurities, 4BV of ethanol with a concentration of 70% is eluted, and the ethanol eluate with a concentration of 70% is collected, and the ethanol is recovered under reduced pressure. The total flavone extract was obtained by drying under reduced pressure, the final t...

Embodiment 3

[0039] Extraction: Take kidney tea (also known as: cat's beard grass leaves, purchased from Tongrentang Pharmaceutical Co., Ltd.) powder with a mesh number of 10, add 80% ethanol aqueous solution at a liquid-to-material ratio of 25L / Kg, and soak at room temperature for 24 hours Stir while soaking, 280W ultrasonic (ultrasonic instrument model is UC-10, purchased from Shanghai Titan Technology Co., Ltd.) treatment for 30min, the obtained extract is filtered with medium-speed quantitative filter paper (pore size 20-50μm), and the filtrate is concentrated by rotary evaporation get a paste;

[0040] Purification: The above paste is separated by AB-8 macroporous adsorption resin column, 2BV of water is eluted to remove impurities, 2BV of ethanol with a concentration of 70% is eluted, and the ethanol eluate with a concentration of 70% is collected, and the ethanol is recovered under reduced pressure. The total flavonoid extract was obtained by drying under reduced pressure, and the f...

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Abstract

The invention discloses an extraction method of a clerodendranthus spicatus total flavone extract. The method comprises the following steps: adding an ethanol aqueous solution with a liquid-material ratio of 5-25 L / Kg and a concentration of 0-95% into a clerodendranthus spicatus powder, performing soaking at room temperature for 1-48h with stirring, performing ultrasonic treatment, filtering the obtained extract liquid, and concentrating filtrate to obtain a paste; and separating the paste with weak-polar macroporous adsorbent resin columns, performing elution with 2-4BV water to remove impurities, performing elution with 2-4BV ethanol with a concentration of 0-95%, collecting ethanol eluent, recovering ethanol under reduced pressure, and performing drying under reduced pressure to obtaintotal flavone extract. The experimental results show that the total flavone extract of the kidney tea can promote serum creatinine and urea nitrogen levels of mice to be obviously reduced, the calciumcontent of kidney tissues to be obviously reduced, and the crystal deposition of calcium oxalate in kidney tissues and the pathological tissue injury of the kidney to be obviously reduced. The clerodendranthus spicatus total flavone extract has effect in preventing or treating urinary calculus, and can be used for preparing medicines, health products or foods for preventing or treating urinary calculus.

Description

technical field [0001] The invention relates to the technical fields of medicine, food and health products, in particular to a method for extracting total flavonoids of kidney tea. Background technique [0002] Urolithiasis, also known as urolithiasis, is the most common disease among urological patients. Stones can occur anywhere in the kidneys, bladder, ureters, and urethra. Clinical symptoms include abdominal and lower back pain, hematuria, frequent urination, urgency, and painful urination, which can cause urinary tract obstruction, urethral injury, hydrops, infection, and impaired renal function. A large number of epidemiological data show that urinary stones are related to many factors such as heredity, age, sex, eating habits, nutritional status, urinary tract infection, environment, metabolic abnormalities, and dyslipidemia. This is a global common disease, and the incidence rate in European and American countries is 5%-12% (Assimos DG. 1089-1090), the number of pa...

Claims

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Application Information

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IPC IPC(8): A61K36/53A61P13/04A23L33/105
CPCA23V2002/00A61K36/53A61K2236/333A61K2236/55A23L33/105A61P13/04A23V2200/30A23V2300/14A23V2300/48
Inventor 董昕晁玉凡李娜高松燕赵红霞王庭芳
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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