Escherichia coli detection kit as well as preparation method and application thereof

A detection kit and Escherichia coli technology, applied in biochemical equipment and methods, methods based on microorganisms, measurement/testing of microorganisms, etc., can solve problems such as inconvenient operation, long detection time, and large limitations in the field of use. To achieve the effect of improving detection efficiency

Pending Publication Date: 2019-03-29
长春万成生物电子工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two products need to be sampled during use. Using a constant temperature incubator to cultivate for 24 hours is not only inconvenient to operate, but also takes a long time to detect (more than 24 hours). Both products are prepared using the principle of microbial

Method used

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  • Escherichia coli detection kit as well as preparation method and application thereof
  • Escherichia coli detection kit as well as preparation method and application thereof
  • Escherichia coli detection kit as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0031] Example 1: The kit of the present invention

[0032] 1. Lysis solution

[0033] Add water to prepare the lysate according to the concentration in any row in Table 1.

[0034] Table 1

[0035]

[0036]

[0037] 2. Test strips

[0038] Weigh 0.02 g of X-GAL, add 0.02 g of polyvinylpyrrolidone, and add 100 mL of absolute ethanol to obtain a color developing solution.

[0039] Prepare neutral chromatography filter paper or any other type of test paper, soak the test paper in the color developing solution prepared above, and dry it at a temperature between room temperature and 100°C to obtain a color block; cut the color block into (2-6mm) )×(2-8mm) is fixed to one end of the substrate through double-sided tape to obtain a test strip. The structure diagram is shown in figure 1 .

[0040] 3. Pack the test strip, lysis solution, reaction tube, straw, and desiccant into a kit.

Example Embodiment

[0041] Example 2: Detection test of the kit of the present invention

[0042] Select E. coli bacteria liquid with an OD value of 0.3, sterile water, mold bacteria liquid with an OD value of 0.3, and Staphylococcus bacteria liquid with an OD value of 0.3 as the test objects for testing;

[0043] Add the lysis solution 4 in Example 1 to the water sample to be tested, and let it stand for 10-20 minutes; then use the test strip in the kit to immerse the test strip in the water sample to be tested and keep it for 10 minutes, if the test strip shows Any degree of blue is positive, and the test strip is colorless or other colors are negative.

[0044] The test results are shown in Table 2 and figure 2 ;

[0045] Table 2

[0046] Sample name

[0047] From the above results, it can be seen that only the Escherichia coli bacteria liquid changes color and shows blue, and the other three groups have no color development. At the same time, the whole process only takes 20min (10min lysis+10min r...

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Abstract

The invention relates to the technical field of water quality microbiological detection and discloses an escherichia coli detection kit as well as a preparation method and an application thereof. Thekit comprises a lysate and a detection test strip, wherein the lysate is prepared from a dehydrant, a penetrant and a surfactant, and the dehydrant is sodium chloride, EDTA-2Na or sodium hydroxide; the penetrant is glycerin, polyethylene glycol 2000, Tween80 or triton-100; the surfactant is polyvinylpyrrolidone or ethyl cellulose; a color developing block is arranged at one end of the detection test strip and contains X-GAL. With the adoption of a dry chemical test paper method, escherichia coli cracks to release galactase and is subjected to a reaction with X-GAL (a galactoside color developing agent) to show blue color, so that dry chemical test paper is prepared; the overall detection process can be shortened to be within 30 min through combination with the specific rapid lysate, and compared with at least 24 h detection time of an existing product, detection efficiency of the escherichia coli in water and food is remarkably improved.

Description

technical field [0001] The invention relates to the technical field of water quality microorganism detection, in particular to an Escherichia coli detection kit and a preparation method and application thereof. Background technique [0002] Escherichia coli is usually called Escherichia coli, which was discovered by Escherich in 1885. Escherichia coli is a Gram-negative bacterium with a size of 0.5×1-3 microns, perinatal flagella, can move, and has no spores. It can ferment a variety of sugars to produce acid and gas. It is a normal resident bacterium in the intestinal tract of humans and animals. Babies enter the intestinal tract with breastfeeding after birth and accompany people for life, accounting for almost 1 / 3 of the dry weight of feces. [0003] The state stipulates that the total number of colonies per milliliter of drinking water is less than 100, and the total coliform group must not be detected in every 100 milliliters of water. [0004] The number of coliform b...

Claims

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Application Information

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IPC IPC(8): C12Q1/34C12Q1/10C12R1/19
CPCC12Q1/10C12Q1/34
Inventor 赵钢唐春王竑婷
Owner 长春万成生物电子工程有限公司
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