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Test paper, manufacturing method, and test method

A technology for detecting test paper and manufacturing methods, which is applied in the direction of measuring devices, instruments, and material analysis through optical means, and can solve the problems of complex environmental sewage samples, interference of coexisting organic substances, and instability of complexes, and achieve the goal of overcoming coexisting pollutants interference, more active sites, and the effect of eliminating the pretreatment process

Inactive Publication Date: 2019-04-12
FOCUSED PHOTONICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] 1. Spectrophotometry, the complex formed in the spectrophotometry is very unstable in aqueous solution, and the reagent used is an organic solvent, so there is a problem of secondary recovery;
[0005] 2. The bromination volumetric method has low sensitivity and is only suitable for the determination of high-concentration phenolic wastewater;
[0006] 3. Chromatography; although the sensitivity is high, the cost is high, and the phenolic compounds in the water sample need to be pretreated, the process is cumbersome and time-consuming
[0007] In addition, the environmental sewage samples are complex, and when the above methods are used for detection, there are interference problems of coexisting organic substances

Method used

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  • Test paper, manufacturing method, and test method

Examples

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Effect test

Embodiment 1

[0033] A kind of detection test paper of the embodiment of the present invention, described detection test paper comprises:

[0034] Hydrophilic nanofibrous membrane;

[0035] an enzyme, the enzyme is immobilized on the hydrophilic nanofiber membrane; the enzyme is suitable for specific reaction with the detection object;

[0036] methylene blue, the methylene blue is adsorbed in the pores of the hydrophilic nanofibrous membrane.

[0037] In order to immobilize the enzyme on the hydrophilic nanofiber membrane, further, the enzyme is immobilized on the hydrophilic nanofiber membrane by a cross-linking method.

[0038] In order to apply the detection test paper to the detection of phenolic compounds, further, the enzyme is laccase, and the detection object is phenolic compounds.

[0039] figure 1 The flow chart of the manufacturing method of the detection test paper of the embodiment of the present invention is schematically given, as figure 1 Shown, the manufacture method o...

Embodiment 2

[0059] An application example of the detection test paper according to Example 1 of the present invention in the detection of phenolic compounds in sewage.

[0060] In this application example, the manufacturing method of the detection test paper comprises the following steps:

[0061] (A0) Obtaining the nanofiber membrane, the acquisition method is:

[0062] Organic soluble polymers or molten polymers are prepared by electrospinning technology under electric field;

[0063] The polymer is at least one of polyvinyl alcohol, polyethylene oxide, polyacrylic acid, polyacrylamide, polyacrylonitrile, and polyvinylidene fluoride, and the concentration of the polymer solution is 0.5 to 15% by weight;

[0064]The requirements of the electrospinning are: the electric field voltage is 12-22kV, such as 12, 18, 22kV; the inner diameter of the syringe needle is 0.40-2.00mm, such as 0.40, 1.50, 2.00mm; the boosting speed is 0.5-2.5mL / h , such as 0.5, 1.9, 2.5mL / h; the spray distance is 5-...

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Abstract

The invention provides a test paper, a manufacturing method, and a test method using the test paper. The test paper comprises a hydrophilic nanofiber membrane, an enzyme, and methylene blue. The enzyme is immobilized on the hydrophilic nanofiber membrane and is suitable for a specific reaction with a test object. The methylene blue is adsorbed in the pores of the hydrophilic nanofiber membrane. The test paper has the advantages of simple preparation, high detection sensitivity and the like.

Description

technical field [0001] The invention relates to the detection of phenolic compounds, in particular to a detection test paper, a manufacturing method and a detection method of phenolic compounds in water. Background technique [0002] The pollution of phenolic compounds is very serious in our country, and the timely and accurate determination of the concentration of phenolic compounds in the environment is the basis and premise for the prevention, control and treatment of phenolic compound pollution. [0003] The detection methods of phenols in the national standard methods are: [0004] 1. Spectrophotometry, the complex formed in the spectrophotometry is very unstable in aqueous solution, and the reagent used is an organic solvent, so there is a problem of secondary recovery; [0005] 2. The bromination volumetric method has low sensitivity and is only suitable for the determination of high-concentration phenolic wastewater; [0006] 3. Chromatography; although the sensiti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N33/544G01N33/543G01N33/531
CPCG01N21/78G01N33/531G01N33/54306G01N33/544G01N2021/7759
Inventor 崔婧嫄陈侠胜胡清泉薛凯元王培城
Owner FOCUSED PHOTONICS
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