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Kit for measurement of apolipoprotein C3 by latex turbidimetric inhibition immuno assay

A technology of apolipoprotein and latex immunization, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of low specificity, affecting reagent sensitivity and stability, etc., achieve good stability, improve sensitivity and stability , the effect of improving accuracy

Inactive Publication Date: 2019-04-12
中拓生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are two commonly used methods for preparing latex-antibody protein complexes, physical adsorption method and chemical coupling method. The physical adsorption method will cause partial analysis and structural changes of latex microsphere surface proteins. The prepared latex-antibody protein complexes The specificity is low, and it is easily interfered by other substances (such as rheumatoid factor and heterophile antibody), and its application in biological diagnosis is limited. The chemical coupling method can control the problems in the adsorption to the greatest extent, but the chemical coupling method The Fab fragment and Fc of the linked antibody are random, which affects the sensitivity and stability of the reagent

Method used

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  • Kit for measurement of apolipoprotein C3 by latex turbidimetric inhibition immuno assay
  • Kit for measurement of apolipoprotein C3 by latex turbidimetric inhibition immuno assay
  • Kit for measurement of apolipoprotein C3 by latex turbidimetric inhibition immuno assay

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] A reagent kit for the determination of apolipoprotein C3 with high sensitivity and good stability, which includes reagent R1, reagent R2, and calibrator.

[0061] The composition of reagent R1 is:

[0062] Phosphate buffer 160mmol / L

[0063] Sodium chloride 140mmol / L

[0064] Triton 100 1 g / L

[0065] PEG6000 52g / L

[0066] Trehalose 1 g / L

[0067] Sodium azide 1 g / L

[0068] pH 7.5

[0069] The composition of reagent R2 is:

[0070] Phosphate buffer 150mmol / L

[0071] Goat anti-human apolipoprotein C3 antibody latex particles 42ml / L

[0072] Sodium chloride 120mmol / L

[0073] Triton 100 1 g / L

[0074] Bovine serum albumin 1 g / L

[0075] Sodium azide 1 g / L

[0076] pH 8.0

[0077] The composition of the calibrator is:

[0078] Phosphate buffer 160mmol / L

[0079] Recombinant Apolipoprotein C3 13mg / dl

[0080] Bovine serum albumin 1g / L

[0081] Sorbitol 1g / L

[0082] Sodium azide 1g / L

[0083] pH 7.5

[0084] The preparation method of the apolipoprotein C3 determination kit includes the following ste...

Embodiment 2

[0090] The composition of reagent R1 is:

[0091] Phosphate buffer 160mmol / L

[0092] Sodium chloride 100mmol / L

[0093] brij-35 1 g / L

[0094] PEG6000 50g / L

[0095] Trehalose 1 g / L

[0096] Sodium azide 1 g / L

[0097] pH 7.5

[0098] The composition of reagent R2 is:

[0099] Phosphate buffer 120mmol / L

[0100] Goat anti-human apolipoprotein C3 antibody latex particles 40ml / L

[0101] Sodium chloride 120mmol / L

[0102] brij-35 1 g / L

[0103] Propylene glycol 1 g / L

[0104] Sodium azide 1 g / L

[0105] pH 8.0

[0106] The composition of the calibrator is:

[0107] Phosphate buffer 160mmol / L

[0108] Recombinant Apolipoprotein C3 15mg / dl

[0109] Bovine serum albumin 1g / L

[0110] Sorbitol 1g / L

[0111] Sodium azide 1g / L

[0112] pH 7.5

[0113] The preparation method is the same as in Example 1.

Embodiment 3

[0115] The composition of reagent R1 is:

[0116] HEPES buffer 160mmol / L

[0117] Magnesium chloride 145mmol / L

[0118] brij-35 1 g / L

[0119] PEG6000 50g / L

[0120] Trehalose 1 g / L

[0121] Sodium azide 1 g / L

[0122] pH 7.5

[0123] The composition of reagent R2 is:

[0124] HEPES buffer 120mmol / L

[0125] Goat anti-human apolipoprotein C3 antibody latex particles 35ml / L

[0126] Magnesium chloride 130mmol / L

[0127] brij-35 1 g / L

[0128] Propylene glycol 1 g / L

[0129] Sodium azide 1 g / L

[0130] pH 8.0

[0131] The composition of the calibrator is:

[0132] HEPES buffer 160mmol / L

[0133] Recombinant Apolipoprotein C3 15mg / dl

[0134] Bovine serum albumin 1g / L

[0135] Sorbitol 1g / L

[0136] Sodium azide 1g / L

[0137] pH 7.5

[0138] The preparation method is the same as in Example 1.

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Abstract

The invention discloses a kit for measuring apolipoprotein C3. The kit comprises a reagent R1, a reagent R2 and a calibrator. The R1 comprises a buffer solution, an electrolyte, a surfactant, a reaction enhancer, a stabilizer, and a preservative. The R2 comprises a buffer solution, sheep anti-human apolipoprotein C3 antibody latex particles, an electrolyte, a surfactant, a stabilizer, and a preservative. The calibrator comprises a buffer solution, recombinant apolipoprotein C3, bovine serum albumin, mannitol, and sodium azide. The apolipoprotein C3 kit is a liquid double-reagent, reconstitution preparation is not required, and therefore the reagents can be directly used when taken out from a bottle. Stability of the obtained latex particles is high. Accuracy of detecting result is greatlyimproved. The sugar residues of the Fc end of the apolipoprotein C3 antibody are oxidized to aldehyde by sodium periodate. The binding rate and stability of the antibody are improved. The active region of antibody binding to antigen is effectively protected. Sensitivity and stability of the detecting reagents are improved.

Description

Technical field [0001] The invention relates to the field of medical immune in vitro diagnostic reagents, in particular to a kit for determining apolipoprotein C3 by using a latex immunoturbidimetric method. Background technique [0002] Apolipoprotein is a huge family, including A, B, C, E and other families. Apolipoprotein is a special kind of protein. In the process of lipid transport, it must be combined with apolipoprotein before it can be transported normally. [0003] Apolipoprotein C3 is a glycoprotein composed of 79 amino acids. It has the highest concentration in the C group. It mainly regulates the decomposition and metabolism of triacylglycerol lipoproteins (TRLs) in hypertriglyceridemia, and is mainly rich in triacylglycerols. There are very few lipoproteins (TRLs), including very low-density lipoproteins and chylomicrons, and very few exist in high-density lipoproteins. Apolipoprotein is mainly synthesized in the liver, and a small part is synthesized in the intestin...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6803
Inventor 解苇生王飞刘安娜隗勇
Owner 中拓生物有限公司
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