Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A live coccidiosis vaccine and its application

A live vaccine, chicken coccidiosis technology, applied in the field of livestock and poultry disease prevention and control, can solve the problem of continuous use for about 10 days for 2-3 days, restrictions on the promotion and use of chicken coccidiosis vaccine, and the inability to compensate for weight before slaughter, etc. problems, achieve good immune protection, improve safety, and reduce damage

Active Publication Date: 2022-08-09
CHINA AGRI UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Utilization of coccidiosis vaccine immunization to prevent and control coccidiosis mainly has the following problems: on the one hand, chickens need to repeatedly ingest the offspring oocysts of the vaccine in the litter, that is, through 2-3 booster immunizations, to establish a good and even Complete immunity against Eimeria tenella infection, the entire immunization process takes 4-5 weeks or even longer, resulting in the inability of young broilers to compensate for the weight loss caused by such enhanced immunization before slaughter; on the other hand , Before a good immunity has been established, that is, 1 week after immunization, chickens ingest a large amount of vaccine progeny oocysts in the feces, which may cause coccidiosis due to vaccine immunization, resulting in the need to wait 10 days after immunization. Continuously use anticoccidial drugs for 2-3 days every day to reduce the side effects of coccidiosis vaccine
The above problems have become a difficult problem in the application of chicken coccidiosis vaccines, and are the key bottlenecks that limit the popularization and use of chicken coccidiosis vaccines, especially in the prevention and control of coccidiosis in broilers

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A live coccidiosis vaccine and its application
  • A live coccidiosis vaccine and its application
  • A live coccidiosis vaccine and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 Influence of co-infection of virulent strains and attenuated strains of coccidia on the pathogenicity of virulent strains of coccidia 1

[0034] In this example, the highly pathogenic species Eimeria tenella commonly used in chicken coccidiosis vaccine components is used as an example, and a high dose of Eimeria tenella that has the same parasitic site in the parasitic life history as Eimeria tenella is used as an example. The less pathogenic species Eimeria lentis co-infected flocks with low doses of the strongly pathogenic species Eimeria tenella. The effect of Eimeria lentis on the pathogenicity of Eimeria tenella was analyzed by oocyst excretion, cecal lesion detection and body weight change.

[0035] 200 1-week-old SPF chickens were randomly divided into 4 groups: non-infected control group (Ctrl), Eimeria tenella infection group (Et), Eimeria tenella infection group (Emi) and Eimeria tenella Coccidia infection group and co-infection group with Eimeri...

Embodiment 2

[0040] Embodiment 2 Influence of co-infection of virulent strains of coccidia with virulent strains on the pathogenicity of virulent strains of coccidia 2

[0041] In this example, Eimeria necatrix, a highly pathogenic species commonly used in chicken coccidiosis vaccine components, is used as an example. The weak pathogenic species of Eimeria lentigo and the low dose of strong pathogenic species of the same parasitic site co-infected chickens. The effect of Eimeria lentis on the pathogenicity of Eimeria viridis was analyzed by oocyst excretion, cecal lesion detection and body weight change.

[0042] 200 1-week-old SPF chickens were randomly divided into 4 groups: non-infected control group (Ctrl), Eimeria poisoning group (En), mild Eimeria infection group (Emi) and Eimeria poisoning group worm infection group and co-infection group with Eimeria lentigo (EnEmi). Each chicken in the above groups was orally inoculated with 200 μL of PBS, 200 μL of PBS solution containing 200 s...

Embodiment 3

[0047] Embodiment 3 Chicken coccidiosis live vaccine and its immune effect analysis

[0048] The present embodiment provides a live vaccine for chicken coccidiosis, which is composed as follows: 200 oocysts / feather of Eimeria tenella, 500 oocysts / feather of Eimeria maxima, and Eimeria giant 200 oocysts / feather, and 1000 oocysts / feather for Eimeria mildew.

[0049] The present embodiment also provides the preparation method of the above-mentioned chicken coccidiosis live vaccine, which is as follows:

[0050] (1) Passing the Eimeria tenella strain Beijing strain, Eimeria pilosula Shandong strain, Eimeria gigantic strain Beijing strain and Eimeria lentiscus Zhuozhou strain respectively through SPF chickens, and harvesting the feces Oocysts, aseptically treated after sporulation;

[0051] (2) Count each coccidia oocyst using a McMaster counter, according to Eimeria tenderloin oocyst: Eimeria pilosa oocyst: Eimeria giant oocyst: mild Eimeria oocysts = 2:5:2:10 ratio, and dilute...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of prevention and control of livestock and poultry diseases, in particular to a live coccidiosis vaccine and its application. The vaccine provided by the present invention comprises a virulent coccidia strain and a coccidia attenuated strain which has the same parasitic site or has the same parasitic site in any one or more stages of the parasitic life cycle; The ratio of the number of oocysts of the attenuated strain and the virulent strain of the coccidia is 50:1 to 3:1. The present invention utilizes a high dose of weakly pathogenic coccidia strains to produce a competitive space occupation advantage for strongly pathogenic strains with the same parasitic site, establishes the space occupation effect of comorbid pathogens in vaccine components, and reduces strong pathogenicity. The fecundity, pathogenic effects, and damage to the host caused by re-intake of viral strains following immunization as vaccine components. The coccidia vaccine provided by the invention can effectively improve the safety of the vaccine and reduce the cost of drug prevention and control under the condition of ensuring the immune efficacy of the vaccine, and has good application potential.

Description

technical field [0001] The invention relates to the technical field of prevention and control of livestock and poultry diseases, in particular to a live coccidiosis vaccine and its application. Background technique [0002] Eimeria is a kind of eukaryotic pathogen that seriously endangers the health of livestock and poultry, and can infect a variety of animals. In the breeding industry, the coccidiosis of chickens and rabbits is the most serious, which greatly affects the breeding efficiency and is one of the main diseases that restrict the development of large-scale chicken and rabbit breeding. Taking chicken coccidiosis as an example, it can cause more than 2 billion US dollars in economic losses every year. At present, the prevention of coccidiosis mainly relies on drugs and vaccines. At present, 13 of the 26 medicated feed additives announced in China are used for the prevention and control of coccidiosis. It can be seen that anti-coccidial drugs occupy "half of the co...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/012A61P33/02
CPCA61K39/012A61P33/02A61K2039/522A61K2039/542A61K2039/552Y02A50/30
Inventor 索勋汤新明刘贤勇索静霞王超越刘婕张思新段春慧
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com