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Live coccidiosis vaccine and application thereof

A coccidiosis and vaccine technology, applied in the field of livestock and poultry disease prevention and control, can solve problems such as continuous use for 2-3 days in about 10 days, restrictions on the promotion and use of coccidiosis vaccines in chickens, and the inability to compensate for weight before slaughtering, etc. Achieve the effect of maintaining immune efficacy, good immune protection, and reducing fecundity

Active Publication Date: 2019-04-19
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Utilization of coccidiosis vaccine immunization to prevent and control coccidiosis mainly has the following problems: on the one hand, chickens need to repeatedly ingest the offspring oocysts of the vaccine in the litter, that is, through 2-3 booster immunizations, to establish a good and even Complete immunity against Eimeria tenella infection, the entire immunization process takes 4-5 weeks or even longer, resulting in the inability of young broilers to compensate for the weight loss caused by such enhanced immunization before slaughter; on the other hand , Before a good immunity has been established, that is, 1 week after immunization, chickens ingest a large amount of vaccine progeny oocysts in the feces, which may cause coccidiosis due to vaccine immunization, resulting in the need to wait 10 days after immunization. Continuously use anticoccidial drugs for 2-3 days every day to reduce the side effects of coccidiosis vaccine
The above problems have become a difficult problem in the application of chicken coccidiosis vaccines, and are the key bottlenecks that limit the popularization and use of chicken coccidiosis vaccines, especially in the prevention and control of coccidiosis in broilers

Method used

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  • Live coccidiosis vaccine and application thereof
  • Live coccidiosis vaccine and application thereof
  • Live coccidiosis vaccine and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Coccidia virulent strain and attenuated strain co-infection affect the pathogenicity of coccidia virulent strain 1

[0034] This embodiment takes Eimeria tenella, a highly pathogenic insect species commonly used in chicken coccidiosis vaccine components, as an example, using a high dose of Eimeria tenella that has the same parasitic site in the parasitic life history The weak pathogenic species of Eimeria tenella co-infected chickens with low doses of the highly pathogenic species of Eimeria tenella. The influence of Eimeria lentilis on the pathogenicity of Eimeria tenella was analyzed by oocyst excretion, detection of cecal lesions, and body weight changes.

[0035] 200 1-week-old SPF chickens were randomly divided into 4 groups: non-infected control group (Ctrl), Eimeria tenella-infected group (Et), Eimeria pallidum-infected group (Emi) and Eimeria tenella Coccidia infection group and Eimeria placida co-infection group (EtEmi). Each chicken of each of the ...

Embodiment 2

[0040] Embodiment 2 coccidia virulent strain and attenuated strain co-infection are to the impact of the pathogenicity of coccidia virulent strain 2

[0041] In this embodiment, the strong pathogenic insect species poisoning Eimeria (Eimeria atrix) commonly used in chicken coccidiosis vaccine components is taken as an example, and high-dose Eimeria coccidia and poisonous Eimeria coccidia have the same characteristics in the parasitic life cycle as examples. Chicken flocks were co-infected by the weak pathogenic species Eimeria pallidum and the low dose of strong pathogenic species Eimeria venom in the same parasitic site. The influence of Eimeria bradymis on the pathogenicity of Eimeria catarrh by oocyst excretion, detection of cecal lesions, and analysis of body weight changes.

[0042] 200 1-week-old SPF chickens were randomly divided into 4 groups: non-infected control group (Ctrl), poisonous Eimeria infection group (En), mild Eimeria infection group (Emi) and poisonous Eim...

Embodiment 3

[0047] Embodiment 3 chicken coccidiosis live vaccine and its immune effect analysis

[0048] The present embodiment provides a kind of live chicken coccidiosis vaccine, and its composition is as follows: Eimeria tenella 200 oocysts / feather part, pile type Eimeria 500 oocysts / feather part, Eimeria maxima 200 oocysts / feather, 1000 oocysts / feather for Eimeria mildew.

[0049] Present embodiment also provides the preparation method of above-mentioned chicken coccidiosis live vaccine, specifically as follows:

[0050] (1) Eimeria tenella Beijing strain, Eimeria heap type Eimeria Shandong strain, Eimeria giga Beijing strain and Eimeria placidus Zhuozhou strain were subcultured respectively through SPF chickens, and harvested feces Oocysts, aseptically processed after sporulation;

[0051] (2) Count each coccidia oocyst using a McMaster counting board, according to Eimeria tenacia oocysts: pile type Eimeria oocysts: Eimeria giant oocysts: mild Eimeria oocysts=2:5:2:10 ratio, and d...

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Abstract

The invention relates to the technical field of prevention and control of livestock and poultry diseases, in particular to a live coccidiosis vaccine and application thereof. The vaccine comprises a coccidiosis virulent strain and a coccidiosis attenuating strain having the same parasitic site or having the same parasitic site at any one or more stages of the parasitic life history; in the vaccine, the oocysts number ratio of the coccidiosis attenuated strain to the coccidiosis virulent strain is 50:1-3:1. A high dose of the weakly pathogenic coccidiosis strain is used for producing a competitive occupying advantage for a highly pathogenic strain having the same parasitic site, a occupying effect of the symbiotic pathogen in vaccine components is established, and the fertility, pathogenicity and damage to a host caused by over-intake are reduced after the highly pathogenic strain is immunized as the vaccine components. The coccidiosis vaccine can effectively improve the safety of the vaccine, reduce the cost of drug prevention and control, and have a good application potential while ensuring the immune efficacy of the vaccine.

Description

technical field [0001] The invention relates to the technical field of livestock and poultry epidemic prevention and control, in particular to a live coccidiosis vaccine and its application. Background technique [0002] Eimeria is a kind of eukaryotic pathogen that seriously endangers the health of livestock and poultry, and can infect a variety of animals. In the breeding industry, coccidiosis in chickens and rabbits is the most serious, which greatly affects the breeding benefits, and is one of the main diseases that restrict the development of large-scale chicken and rabbit breeding industries. Take chicken coccidiosis as an example, which can cause economic losses of more than 2 billion US dollars every year. At present, the prevention of coccidiosis mainly relies on drugs and vaccines. At present, 13 of the 26 drug feed additives published in China are used for the prevention and control of coccidiosis. It can be seen that anticoccidial drugs account for "half of the...

Claims

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Application Information

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IPC IPC(8): A61K39/012A61P33/02
CPCA61K39/012A61P33/02A61K2039/522A61K2039/542A61K2039/552Y02A50/30
Inventor 索勋汤新明刘贤勇索静霞王超越刘婕张思新段春慧
Owner CHINA AGRI UNIV
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