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Process for preparing viral and bacterial vaccine for animals utilizing vegetable oil adjuvant

A vegetable oil, viral technology, applied in the direction of antibacterial drugs, antiviral agents, viral antigen components, etc., can solve the problems of slow weight gain of young animals, unfavorable development of animal husbandry, etc., so as not to affect immune efficacy and reduce adverse reactions. Effect

Inactive Publication Date: 2005-09-21
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing technology, mineral oil adjuvants are more common in the application of animal vaccines, but in recent years, in the immune prevention of animal mineral oil adjuvant vaccines, due to frequent immune reactions, some of the animals injected High fever, food reduction, food waste, miscarriage, and production performance decline, such as reduced milk production of dairy animals, slowed weight gain of young animals and other adverse reactions, especially the death of a very small number of animals due to allergic reactions, which is not conducive to the development of animal husbandry develop

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] This embodiment is the animal virus subtype avian influenza H5N1 and H9N2 vegetable oil adjuvant inactivated mixed vaccine prepared by utilizing vegetable oil adjuvant, and its preparation method comprises the following steps:

[0025] (1) making an antigenic agent, replicating the vaccine strain with sensitive cells or culture medium, after inactivation, obtaining the antigen and making the antigen into an antigenic agent; wherein:

[0026] 1) Virus strains: popular strains H5N1 and H9N2 (1:1 ratio) for seedling production, biological characteristics of seedling strains: chicken embryo half infectious dose (EID 50 ) is 8.25log2 / ml, that is, the virus content of seed virus is 8.25 EID per ml 50 Chicken embryo mean death time (MDT) is 72 hours after the allantoic cavity is inoculated with 0.1ml; Hemagglutination (HA) titer is to the HA≥8log2 of 1% SPF chicken erythrocyte before the chick embryo allantoic fluid of harvest is inactivated; The degree is free from any forei...

Embodiment 2

[0034] Present embodiment is the porcine type O foot-and-mouth disease inactivated vaccine that utilizes vegetable oil adjuvant to prepare, and its preparation method comprises the following steps:

[0035] (1) making an antigenic agent, replicating the vaccine strain with sensitive cells or culture medium, after inactivation, obtaining the antigen and making the antigen into an antigenic agent; wherein:

[0036] 1) Virus strain: The vaccine strain is porcine O-type foot-and-mouth disease virus OZ strain

[0037] The biological characteristics of the seedling strain: the seedling strain should conform to the characteristics of O-type foot-and-mouth disease virus. Suckling rat poison is poisoned by 6-8-day-old suckling mice, 4-5 generations of suckling mice with O-type foot-and-mouth disease porcine blister skin poison, diluted with phosphate buffer solution of pH 7.6-7.8, and subcutaneously injected on the back of the neck for 3 - 0.2ml / mouse for 4-day-old suckling rats, each...

Embodiment 3

[0047] Present embodiment is the bovine type O foot-and-mouth disease inactivated vaccine that utilizes vegetable oil adjuvant to prepare, and its preparation method comprises the following steps:

[0048] (1) making an antigenic agent, copying the vaccine-producing virus (bacteria) strain with sensitive cells or medium, after inactivation, obtaining the antigen and making the antigen into an antigenic agent; wherein:

[0049] 1) Virus strain: The seedling strain is bovine O-type foot-and-mouth disease virus Akesu / 58 strain Biological characteristics of the seedling strain: The seedling strain should conform to the characteristics of O-type foot-and-mouth disease virus. Carry out 1: 1000 times dilution with PBS (phosphate buffered saline) to virus seed, go up alcohol scratch and smear 2ml of the 2-4 year old cattle that do not have foot-and-mouth disease O type serum antibody, in 24-48 hours, test cattle Typical secondary lesions of FMD should appear.

[0050] Rejuvenation of...

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PUM

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Abstract

A method of producing animal's virus and bacterial vaccine by using vegetable oil adjuvant. It comprises the following steps,(1)make antigen agents, gain the antigens by copying the sprout bacterial strain with the sensitive cells and culture medium, and then deactivating them; produce the antigen agents by the antigens;(2)make vegetable oil adjuvant; (3)mixing, mix the antigen agents and the vegetable oil adjuvant proportional, homogeneous, and finish the vaccine. Wherein, the antigen agents are mixed with antigens and disinfection tuwen-80; the vegetable oil adjuvant is mixed with vegetable oil and span-80. The invention adopts new adjuvant to make the vaccine. When it is applied, it won't affect the immune efficiency of the vaccine, can drop the vaccine's untoward reaction, and is beneficial to produce safety meat foot. Compared to the current techniques, it has evident advantages. The invention is suit to various applications of animal virus and bacterial vaccine.

Description

Technical field: [0001] The invention relates to a method for producing vaccines using vegetable oil adjuvants, in particular to a method for preparing animal viral and bacterial vaccines using vegetable oil adjuvants. Background technique: [0002] Adjuvant, as a substance that can assist antigen to enhance immune response, can help antigen to be released slowly at the injection site to increase the effect of immune stimulation. In the existing technology, mineral oil adjuvants are more common in the application of animal vaccines, but in recent years, in the immune prevention of animal mineral oil adjuvant vaccines, due to frequent immune reactions, some of the animals injected with vaccines High fever, food reduction, food waste, miscarriage, and decline in production performance, such as reduced milk production of dairy animals, slowed weight gain of young animals and other adverse reactions, especially the death of a very small number of animals due to allergic reaction...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/002A61K39/12A61K39/39A61P31/04A61P31/12
Inventor 柳纪省殷相平黄银君白银梅牟克斌李宝玉杨联李志勇王辉兰喜张兴旺
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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