Method for simultaneously separating and purifying coagulation factors IX, X and VII from human plasma

A technology for coagulation factor and simultaneous separation, which is applied to coagulation/fibrinolytic factors, chemical instruments and methods, and peptide preparation methods, etc., can solve the problems of cumbersome steps, long time consumption, etc. The effect of purity
CN109651502AActive Publication Date: 2019-04-19HUALAN BIOLOGICAL ENG INC +2
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HUALAN BIOLOGICAL ENG INC
Publication Date
2019-04-19

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Abstract

The invention discloses a method for simultaneously separating and purifying coagulation factors IX, X and VII from human plasma, which comprises the following steps: performing centrifugal impurity removal, gel adsorption and ultrafiltration concentration to prepare a prothrombin compound; separating the coagulation factors VII and a mixed solution containing IX and II through an anion exchange resin column; separating the coagulation factor II and IX from the mixed solution containing IX and II by affinity chromatography. According to the method, by combining anion exchange chromatography with heparin affinity chromatography, the separation and preparation of three coagulation factors II, VII and IX at the same time can be achieved; the method has the advantages of high raw material utilization, simple operation and short time consumption; meanwhile, by detecting the electric signals in the chromatography process, the corresponding coagulation factors are accurately collected, the purity of the coagulation factors is effectively improved, and the economic benefit is improved.
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Description

technical field

[0001] The invention relates to the technical field of separation and purification of biological macromolecules, in particular to a method for simultaneously separating and purifying blood coagulation factors IX, X and VII from human plasma. Background technique

[0002] Coagulation factors are various protein components involved in the blood coagulation process. They are activated during bleeding, adhere to platelets and plug wounds on blood vessels. They are specific drugs for the treatment of hemophilia. At present, coagulation factors VII, VIII, IX and prothrombin complexes (mainly coagulation factors II, VII, IX, and X) have been developed into drugs for the treatment of hemophilia A or B.

[0003] Coagulation factor IX (FIX for short) is clinically used in the treatment of hemophilia B patients, so further purification of high-purity coagulation factor IX from the prothrombin complex has clinical application value. In addition, blood coagulation factor...

Claims

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