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Extraction process of response surface process optimization uralla total flavonoids and anti-oxidization purpose thereof

A response surface method and the technology of Uralia sativa, which are applied to medical preparations containing active ingredients, anti-toxins, chemical process analysis/design, etc., can solve the problem of less flavonoids in Urastia sativa

Active Publication Date: 2019-04-26
JILIN INST OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been few studies on the flavonoids of Uralia sativa, and there have been no reports on the optimization of the extraction process and the structure and function of the total flavonoids of Urassica sativa by using response surface methodology.

Method used

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  • Extraction process of response surface process optimization uralla total flavonoids and anti-oxidization purpose thereof
  • Extraction process of response surface process optimization uralla total flavonoids and anti-oxidization purpose thereof
  • Extraction process of response surface process optimization uralla total flavonoids and anti-oxidization purpose thereof

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Embodiment 1

[0028] The preferred embodiments of the present invention will be described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0029] (1) Dry and pulverize Uralia ulata to obtain Urla ulati powder, which is passed through a 40-mesh pharmacopoeia sieve.

[0030] (2) Extract the Ulacao powder with ethanol, concentrate and dry the extract, and determine the total flavonoid content of the dry extract.

[0031] (3) Select the four factors of ethanol concentration, liquid-to-solid ratio, extraction time, and extraction temperature to conduct a single-factor experiment on step (2) to determine the optimal levels of ethanol concentration, liquid-to-solid ratio, extraction time, and extraction temperature. The optimal point of the experiment is centered, and one horizontal value is taken around the ...

Embodiment 2

[0062] LC-MS analysis of flavonoids from Uralia pratense

[0063] Preparation of the test solution

[0064] Precisely weigh the appropriate amount of flavonoids from Ulagrass flavonoids, dissolve it with a small amount of acetonitrile to prepare a certain concentration of the sample solution to be tested, and use it for later use. Substances are analyzed and identified.

[0065] Chromatography and mass spectrometry conditions

[0066] Liquid chromatography conditions: Symmetry® C18 (5μm, 4.6×250mm), injection volume 5μL, see Table 4 for mobile phase elution program.

[0067] Mass spectrometry conditions: the ESI source injection voltage was set to 4000 V, the source temperature (TEM) was 300 °C, the volume flow rate of the dry gas was 1 L / min, the pressure of the atomizing gas was 30 psi, the pressure of the auxiliary gas was 60 psi, and the interface was continuously heated. Time-of-flight mass spectrometry detector.

[0068] Table 4 Mobile phase elution system schedule ...

Embodiment 3

[0073] Antioxidant research on total flavonoids of Uralia pratense under optimal extraction parameters

[0074] Take the total flavonoids extract of Uralia sativa extracted under the optimal conditions, measure its flavonoid content as the stock solution of 0.069 mg / mL, configure it as a stock solution of 0.05 mg / mL, and then divide the stock solution every 0.1 mg / mL (remove hydroxyl free Radicals and scavenging ABTS free radicals) and 0.01 mg / mL (scavenging DPPH free radicals) as a gradient, which were successively diluted into 5 concentrations for later use. Take the VC test sample and make it into 0.1 mg / mL VC stock solution, and then dilute the stock solution into 5 concentrations at intervals of 0.2 mg / mL and 0.02 mg / mL as a gradient. Experiments were repeated three times.

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Abstract

The invention provides an extraction process of response surface process optimization uralla total flavonoids and an anti-oxidization function. The ethanol concentration, the material-liquid ratio, the extraction time and the extraction time are used as single factors; the extraction rate (percent) of the total flavonoids is used as a response value; the optimization is performed on the extractionprocess through the response surface design tests; an optimum process realizing the ethanol concentration being 49.40 percent, the material-liquid ratio being 30.49mL / g, the extraction time being 91min, the extraction temperature being 71 DEG C, the highest extraction rate being 6.248 percent, and the uralla total flavonoids practical extraction rate being 6.24 percent+ / - 0.04 percent is realized; the LC-MS combined use is used for qualitative analysis; the condition that uralla possibly contains five kinds of compounds such as catechin, rutin, orientoside, luteolin-7-o-D-glucuronic acid andcyanidenon is speculated; the anti-oxidization activity of the total flavonoids is studied; the condition that the total flavonoids have certain clearing capability for clearing away hydroxide radicals, ABTS and DPPH free radicals is discovered; the IC 50 is respectively 0.232, 0.012 and 0.026mg / mL. The result shows that the obvious anti-oxidization capability is realized.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and relates to a method for optimally extracting total flavonoids from Uralia pratense by response surface method and its anti-oxidation application. Background technique [0002] Carex meyeriana Kunth (Carex meyeriana Kunth) is a perennial herb, Cyperaceae Carex (Carex L.), mainly grows in Heilongjiang, Jilin, Inner Mongolia, Sichuan and other places. ", can actively absorb moisture, has good adsorption capacity, but it is not easy to be corroded, so it can be used all year round. According to literature records, Ulagrass has a unique function of keeping warm, breathable, cold-proof, and antibacterial. The history of using ulagrass itself as a shoe lining can be traced back to hundreds of years ago. In the past, residents in the Northeast used animal fur to make shoes, and waffled the treated ulagrass inside the shoes to make cold-proof shoes. It is the "grass sandals" that the poor p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/89A61P39/06G16C20/10
CPCA61K36/89A61K2236/333A61K2236/51A61P39/06
Inventor 周鸿立王洪彪晏自强
Owner JILIN INST OF CHEM TECH
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