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A microfluidic device for automated transport and posture adjustment of zebrafish larvae

A technology for automatic transportation and attitude adjustment, applied in enzymology/microbiology devices, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the problems of long time-consuming and low success rate of juvenile fish, and achieve convenient experimental observation, Effect of reducing damage and high transparency

Active Publication Date: 2019-10-22
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the existing problems of long time-consuming and low success rate when injecting larvae, and to provide a microfluidic device for automatic delivery and attitude adjustment of zebrafish larvae

Method used

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  • A microfluidic device for automated transport and posture adjustment of zebrafish larvae
  • A microfluidic device for automated transport and posture adjustment of zebrafish larvae
  • A microfluidic device for automated transport and posture adjustment of zebrafish larvae

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Experimental program
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specific Embodiment approach 1

[0033] Specific implementation mode one: the following combination Figure 1-Figure 7 Illustrate this embodiment, the microfluidic channel device of the zebrafish juvenile described in this embodiment that automatically transports and adjusts posture, it includes an upper module, a middle module and a lower module;

[0034] The upper module includes an upper board 1 and a fish feeding device 2;

[0035] The right end of the upper plate 1 is provided with a square groove, and the fish feeding device 2 is embedded in the square groove, and the outlet channel of the square funnel with an inclination angle of 45° is below the square groove;

[0036] The middle module includes a middle plate 3, a valve block 4, a reversing gear 5 and a rack 6;

[0037] The upper part of the reversing gear 5 is a cylindrical boss, on which a reversing microchannel 5-1 is arranged, and a circular hole is arranged in the center of the middle plate 3, and the cylindrical hole of the reversing gear 5 i...

specific Embodiment approach 2

[0043] Specific implementation mode two: the following combination image 3 Illustrate this embodiment, and this embodiment will further explain Embodiment 1. The fish delivery device 2 includes an upper cover 2-1, a fish delivery square tube 2-2 and a sealing ring 2-3; the upper cover 2-1 is slidably installed on the fish delivery At the top opening of the square cylinder 2-2, the top surface of the fish delivery square cylinder 2-2 is provided with a circular groove, the sealing ring 2-3 is installed in the circular groove, and the side of the fish delivery square cylinder 2-2 is provided with a circular groove. There are syringe pump connection holes 2-4.

[0044] In this embodiment, the upper cover 2-1 and the square fish feeding cylinder 2-2 adopt a sliding connection, which can reduce the change of the internal air pressure. The sealing ring 2-3 can increase the sealing performance of the loam cake 2-1 and the fish sending square cylinder 2-2.

specific Embodiment approach 3

[0045] Specific implementation mode three: the following combination figure 2 Describe this embodiment, this embodiment will further explain Embodiment 1, the middle of the upper plate 1 is provided with a calibration cross groove, and the two ends are respectively provided with positioning pin holes; the two ends of the middle plate 3 are provided with positioning pin holes; the lower plate The two sides of the bottom end of the cuboid groove of 7 are provided with positioning pin holes; the upper plate 1, the middle plate 3 and the lower plate 7 are installed together through the corresponding positioning pin holes.

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Abstract

A micro-channel device for automatic conveying and posture adjusting of young zebra fish belongs to the field of microinjection. The device is provided to solve a problem that young fish injection atpresent is long in time and low in success rate. An upper-layer module includes an upper plate and a fish conveying unit. A cylindrical boss is on a reversing gear and is provided with a reversing micro-channel. A middle-layer plate is provided with a micro-channel penetrating the center round hole. The right end of the micro-channel is right below a square hopper outlet passage of the fish conveying unit. When the reversing micro-channel is at a lateral position, the reversing micro-channel and the micro-channel are communicated with each other, and the front part of a valve block is providedwith a square stepped through hole in which a rack is disposed. The upper-layer module and a middle-layer module are mounted in a rectangular groove. A first motor is mounted in a first motor mounting hole. A first motor output shaft is provided with a first gear meshed with the rack. The second gear is meshed with the reversing gear. The device is used for microinjection of young zebra fish.

Description

technical field [0001] The invention relates to a microfluidic device for automatically conveying and adjusting attitude of zebrafish larvae, belonging to the field of microinjection operations. Background technique [0002] As a model animal, zebrafish is playing an increasingly important role in the research fields of life science, health science, and environmental science. The similarity between its genome sequence and that of humans reaches more than 70%, and it is known as the "water mouse". Zebrafish has biological characteristics such as large egg production, rapid development, transparent embryos, in vitro fertilization and in vitro development, allowing researchers to carry out various experiments more efficiently (Genetic Hereditas (Beijing) August 2018, 40(8) :683-692). [0003] Due to the tiny size of juvenile zebrafish (about 3mm long and 0.5mm wide), there are problems such as long time-consuming and low success rate when manually injecting juvenile fish. The...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00
Inventor 佟明斯杨学博庄松霖马奇智林伟阳高会军
Owner HARBIN INST OF TECH
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