Interfering RNA that suppresses Rac1 expression and its application in increasing chemosensitivity of breast cancer

A breast cancer, high-sensitivity technology, applied in the directions of organic active ingredients, inorganic active ingredients, and non-active ingredients medical preparations, etc., can solve the problems of single treatment mode and uncontrollable drug release, and achieve high enrichment, Improved chemosensitivity and longer cycle time

Active Publication Date: 2021-10-01
SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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  • Abstract
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Problems solved by technology

Compared with ordinary chemotherapeutic drugs, nanomedicine changes its metabolism and organ distribution in the body. Nanomedicine can significantly reduce toxic side effects and improve the quality of life of tumor patients while improving the therapeutic effect of tumors; however, the current clinical use of nanomedicine The drug still has disadvantages such as uncontrollable drug release and single treatment method

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  • Interfering RNA that suppresses Rac1 expression and its application in increasing chemosensitivity of breast cancer
  • Interfering RNA that suppresses Rac1 expression and its application in increasing chemosensitivity of breast cancer
  • Interfering RNA that suppresses Rac1 expression and its application in increasing chemosensitivity of breast cancer

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Embodiment

[0037] This embodiment provides a chemotherapeutic drug for breast cancer, including nanocarriers responsive to the acidic pH of endosomes, the nanocarriers are loaded with siRNA and chemotherapeutic drugs, and the siRNA can knock down the expression of the drug resistance gene Rac1 of cancer cells.

[0038] Wherein, the siRNA is an interfering RNA that inhibits the expression of Rac1, and its gene sequence can be 5'-AGACGGAGCTGTAGGTAAA-3' (siRac1-1), as shown in SEQ NO.1, and its gene sequence can also be 5'-CCTTTGTACGCTTTGCTCA-3 '(siRac1-2), as shown in SEQ NO.2.

[0039] In this embodiment, what the nanocarrier adopts is polyethylene glycol-b-poly(2-(diisopropylamino)ethyl methacrylate) (PEG-b-PDPA), and its response pH value is The matrix environment of the cell endosome has a pH value range, that is to say, the response pH value of the nanocarrier is 6.0-6.5. In practical applications, nanocarriers are not limited to PEG-b-PDPA, as long as the response pH is within the pH ...

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Abstract

The present invention relates to the field of biomedicine, and specifically discloses an interfering RNA that inhibits the expression of Rac1 and its application in increasing the chemosensitivity of breast cancer. It also discloses a chemotherapeutic drug for breast cancer, including the above-mentioned interference that inhibits the expression of Rac1 RNA, chemotherapeutic drugs, and tumor microenvironment-responsive carriers for transporting the interfering RNA and the chemotherapeutic drugs. The invention uses acid-sensitive nanomaterials as carriers, which can efficiently transport siRNA and chemotherapeutic drugs into tumor cells, have a longer circulation time in blood, and have a high enrichment amount in tumors; at the same time, siRNA and chemotherapeutic prodrugs are loaded , can efficiently silence target genes, effectively inhibit the expression of drug-resistant genes, and at the same time, enhance the killing of tumor cells, significantly improve the effect of tumor chemotherapy, have little toxic side effects on normal tissue cells, and have no obvious damage to important organs such as the heart and liver.

Description

technical field [0001] The invention relates to the field of biology, in particular to an interfering RNA for inhibiting the expression of Rac1 and its application in increasing the chemosensitivity of breast cancer. Background technique [0002] The incidence of cancer is increasing year by year, and it has become one of the most serious diseases threatening human health. [0003] At present, chemotherapy is still the cornerstone of cancer treatment, but chemotherapy drugs lack targeting, which kills tumor cells and also greatly damages normal cells, which leads to some patients who cannot tolerate the side effects of chemotherapy and discontinue treatment. Moreover, many patients receiving chemotherapy are prone to develop drug resistance after a period of treatment. [0004] At present, some new anti-cancer drugs have been developed, such as molecular targeted drugs, antibody-drug conjugates, RNA interference technology, and nano-drugs. [0005] It has been found that t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7105A61K9/14A61K47/34A61K33/243A61K31/704A61K31/337A61P35/00A61P35/04
Inventor 姚和瑞许小丁胡海李青剑毕卓菲
Owner SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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