Microbial culture solution for promoting soybean growth and preparation method thereof

A technology for microbial culture and culture solution, applied in fertilizer mixtures, fertilization devices, applications, etc., can solve the problems of insignificant overall effect, pollution of farmland soil and air, excessive use of chemical fertilizers, etc., to promote soybean growth, low cost, The effect of simple ingredients

Pending Publication Date: 2019-05-07
YUNNAN AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current soybean production cannot meet the supply demand. Improving soybean production is an urgent problem to be solved. At present, there are two commonly used measures to increase soybean production: 1. Supplementing nitrogen, phosphorus and potassium through fertilization. This method is an important measure to obtain high soybean production. However, at present, the amount of chemical fertilizers used in my country has significantly exceeded the standard, resulting in serious pollution of farmland soil and the atmosphere; 2. The method of intercropping and rotation of soybeans with corn or other plants to increase production, but the overall effect is not obvious
[0004] The fungus Acremonium acremonium has been found to have the activity of killing root-knot nematodes of crops, but there is no report on the effect of promoting soybean growth and improving soybean yield by making culture solution. Acremonium acremonium fungus can be used as a kind of microbial fertilizer, namely Can achieve the effect of promoting soybean growth

Method used

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  • Microbial culture solution for promoting soybean growth and preparation method thereof
  • Microbial culture solution for promoting soybean growth and preparation method thereof
  • Microbial culture solution for promoting soybean growth and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment one: the preparation of a kind of nutrient solution that promotes soybean growth

[0023] Inoculate the fungus Acremonium acremonium on a sterile PDA plate, culture at 28°C for 10 days, scrape the cultured fungus Acremonium acremonium hyphae and spores into 600mL sterile water with an inoculation loop, and mix well, according to Add the required amount of potassium nitrate 0.10-0.12mM, calcium nitrate 0.8-0.11mM, calcium chloride 0.8-1.5mM, magnesium chloride 0.015-0.023mM, mix well, and adjust the pH value to 6-7.8, which can promote the cultivation of soybean growth liquid.

Embodiment 2

[0024] Embodiment two: the influence of nutrient solution on soybean growth

[0025] Soybean is carried out nutrient solution culture, and what use the nutrient solution of embodiment one gain is experimental group, and control group uses clear water, and experimental procedure is as follows:

[0026] 1. Cover the 10L plastic barrel with barbed wire, put 30 healthy soybean seeds on the barbed wire in each barrel, a total of 6 barrels, add water to submerge the seeds, and cultivate them for 15 days to germinate and grow roots;

[0027] 2. Change the clear water nutrient solution, three barrels use the nutrient solution obtained in Example 1, and the three barrels use clear water as a contrast, and the nutrient solution liquid level has not crossed soybean seeds;

[0028] 3. Place 6 barrels of soybeans in a plastic greenhouse for three months at room temperature, add culture medium every 15 days, observe and take photos to record the growth of soybeans at any time;

[0029] 4. ...

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Abstract

The invention relates to the technical field of crop planting, and particularly relates to a microbial culture solution for promoting soybean growth and a preparation method thereof. The microbial culture solution for promoting soybean growth comprises acremonium yunnanensis hyphae and spores and other nutritional substances required by soybean growth, wherein the concentration of the acremonium yunnanensis spores is 10<4>-10<6> spores / mL. The culture solution disclosed by the invention is used for hydroponic planting of soybeans, nutrient substances required by soybean growth are provided, and the yield of the soybeans is increased. The experiment proves that compared with a control group, soybeans cultured by using the culture solution has more flowers, pods and soybean numbers and has better growth vigor. According to the invention, a reference preparation method is provided for the soybean high-yield hydroponic culture solution.

Description

technical field [0001] The invention relates to the technical field of crop planting, in particular to a microbial culture solution for promoting soybean growth and a preparation method thereof. Background technique [0002] Soybeans, also known as soybeans, are native to China and are cultivated everywhere, and are also widely cultivated all over the world. Soybean is one of the important food crops in China. It has been cultivated for 5,000 years. It was called Shu in ancient times. It is mainly produced in Northeast China. Its seeds are rich in plant protein. Soybeans are most commonly used to make various soy products, extract soybean oil, brew soy sauce and extract protein. Okara, or soybeans ground into a meal, are also commonly used in livestock feed. [0003] The current soybean production cannot meet the supply demand. Improving soybean production is an urgent problem to be solved. At present, there are two commonly used measures to increase soybean production: 1....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00
Inventor 杜云龙万媛媛韩丽平翔蕊赵宜婷周璇武丽霞何云露彭晟何霞红
Owner YUNNAN AGRICULTURAL UNIVERSITY
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