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Detection kit for procalcitonin (PCT)

A detection kit and procalcitonin technology, applied in the field of biochemical detection, can solve the problems of narrow linear range, low sensitivity, poor stability, etc., and achieve the effect of wide linear range, strong anti-interference, and improved stability

Active Publication Date: 2019-05-07
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the above problems, the object of the present invention is to provide a procalcitonin (PCT) detection kit, which adopts latex enhanced immune turbidimetric method to overcome the low sensitivity, poor stability and narrow linear range of common immune turbidimetric method. Shortcoming, can effectively detect the content of procalcitonin (PCT), has the advantages such as high sensitivity, good stability, wide linear range; Another object of the present invention is to provide the preparation and the use method of this detection kit

Method used

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  • Detection kit for procalcitonin (PCT)
  • Detection kit for procalcitonin (PCT)
  • Detection kit for procalcitonin (PCT)

Examples

Experimental program
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Embodiment 1

[0033] The procalcitonin (PCT) detection reagent provided in this embodiment includes reagent R1 and reagent R2:

[0034] 1) The components of reagent R1 include:

[0035]

[0036]

[0037] Described reagent R2 comprises the component of following content:

[0038]

[0039] 2) The usage method of the reagent of this embodiment:

[0040] The procalcitonin (PCT) detection kit described in this embodiment adopts a fully automatic biochemical analyzer with dual reagent functions, such as Hitachi 7180 fully automatic analyzer, etc., and uses the endpoint method for determination. Place R1 and R2 on the corresponding reagent positions according to the ratio of 3:1, and place distilled water, standards and samples on the corresponding positions of the sample plate, and the operation is shown in Table 1.

[0041] Table 1 Reagent detection method table

[0042]

[0043]

[0044] Calculation: Procalcitonin (PCT) content (ng / mL) = (ΔA determination ÷ ΔA standard) × C st...

Embodiment 2

[0046] The detection reagent of procalcitonin (PCT) described in this embodiment includes reagent R1 and reagent R2 (detection method is the same as embodiment 1):

[0047] 1) The components of reagent R1 include:

[0048]

[0049] Described reagent R2 comprises the component of following content:

[0050]

[0051]

Embodiment 3

[0053] The detection reagent of procalcitonin (PCT) described in this embodiment includes reagent R1 and reagent R2 (detection method is the same as embodiment 1):

[0054] 1) The components of reagent R1 include:

[0055]

[0056] Described reagent R2 comprises the component of following content:

[0057]

[0058]

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Abstract

The invention relates to the technical field of procalcitonin (PCT) detection, in particular to a detection kit for procalcitonin (PCT) and a preparation and application method thereof. A reagent R1 contains a buffer solution, potassium chloride, ethyl carbamate, 2-hydroxypropyl-Beta-cyclodextrin, sorbitol, PEG-20000, N, N-diethyl-p-octadecylsulfonimidopropylammonium propanesulfonate, and a preservative. A reagent R2 comprise a buffer solution, ethyl carbamate, 2-hydroxypropyl-Beta-cyclodextrin, sorbitol, rabbit anti-human calcitonin (PCT) antibody-coated latex particles (65nm), rabbit anti-human calcitonin (PCT) antibody-coated latex particles (145nm), rabbit anti-human calcitonin (PCT) antibody-coated latex particles (280nm), N, N-diethyl-p-octadecylsulfopropylammonium propanesulfonate, and a preservative. According to the detection kit for procalcitonin (PCT), the stability and the linear range of the reagent are remarkably improved and the sensitivity and accuracy of the reagentare remarkably enhanced.

Description

technical field [0001] The invention belongs to the technical field of biochemical detection, and in particular relates to a procalcitonin (PCT) detection kit and a preparation and use method thereof. Background technique [0002] Procalcitonin (PCT) is a protein whose plasma levels are elevated in severe bacterial, fungal, and parasitic infections, as well as in sepsis and multiorgan failure. Procalcitonin (PCT) does not rise in autoimmunity, allergies, and viral infections. Local limited bacterial infection, minor infection and chronic inflammation do not cause it to be elevated. Bacterial endotoxins played a crucial role in the induction process. [0003] Procalcitonin (PCT) reflects the active degree of systemic inflammatory response. Factors that affect procalcitonin (PCT) levels include the size and type of organ affected, the type of bacteria, the degree of inflammation, and the state of the immune response. In addition, procalcitonin (PCT) can only be measured 1-...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 甘宜梧李志明王美丽
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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