Attenuated swine fever virus live marker vaccine strain and vaccine composition for oral administration using same

A technique of live attenuated vaccine and swine fever virus, which is applied in the field of the attenuated swine fever virus labeled live vaccine strain and the vaccine composition for oral administration using the same, and can solve the problem that it is difficult for pigs to distinguish infection antibodies from field strains, Serological identification and other issues, to achieve the effects of enhanced safety, alleviation of side effects, and excellent effects

Pending Publication Date: 2019-05-21
大韩民国农林畜产食品部农林畜产检疫本部
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] In addition, the LOM vaccine strain also has the problem of difficult serological differentiation, that is, it is difficult to distinguish the infection antibody of the wild strain and the infection antibody of the LOM vaccine strain ...

Method used

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  • Attenuated swine fever virus live marker vaccine strain and vaccine composition for oral administration using same
  • Attenuated swine fever virus live marker vaccine strain and vaccine composition for oral administration using same
  • Attenuated swine fever virus live marker vaccine strain and vaccine composition for oral administration using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] [Example 1] Preparation of attenuated classical swine fever virus marker live vaccine strain CSFV BErns attenuated C-P50

Embodiment 1-1

[0063] [Example 1-1] Cultivating Flc-LOM-Berns virus in CPK cells

[0064] Flc-LOM-Berns virus strain (deposit number: KCTC12304BP) was first prepared by the method described in Korean Laid-Open Patent No. 2010-0121288. In order to increase the titer and attenuate the Flc-LOM-Berns virus, the cells used to subculture the virus were explored. As a result of multiple experiments, compared with PK cells (porcine kidney cells, porcine kidney cells) and BK cells (bovine kidney cells, bovine kidney cells), in CPK cells (cloned porcine kidney cells, cloned porcine kidney cells) The CSFV-marked live virus can be obtained more effectively during infection and subculture, so CPK cells were selected as the first cells to be subcultured.

[0065] In order to further improve the titer of the Flc-LOM-BErns virus, first, the mycoplasma that contaminates the prepared Flc-LOM-BErns virus is removed by the following method. First, add MRA (Mycoplasma removal agent (Mycoplasma removal agent), ...

Embodiment 1-2

[0081] [Example 1-2] Continuous subculture in BK cells, PK cells and CPK cells

[0082] In order to increase the titer of the virus propagated to the 20th generation by culturing in the CPK cells of Example 1-1, in BK cells, the same culture conditions as in Example 1-1 were carried out again from the 21st generation to the 30th generation. Generations of continuous culture, the resulting titer from 1 × 10 3.5 TCID 50 / ml increased by 1×10 1.0 TCID 50 / ml and reached 1×10 4.5 TCID 50 / ml. Afterwards, in PK cells again, with the same culture condition as in Example 1-1, proliferate from the 31st generation to the 40th generation, as a result, the titer ranges from 1×10 4.5 TCID 50 / ml increased by 1×10 1.0 TCID 50 / ml and reached 1×10 5.5 TCID 50 / ml. Finally, go back to CPK cells again, and propagate from 41 generations to 50 generations with the same culture conditions as in Example 1-1, and the resulting titer ranges from 1×10 5.5 TCID 50 / ml increased by 1×10 ...

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Abstract

The present invention relates to an attenuated swine fever virus live marker vaccine strain and a vaccine composition for oral administration using same and, more particularly, to: an attenuated swinefever virus live marker vaccine strain (CSFV BErns attenuated C-P50) obtained by alternately and continuously culturing the conventional swine fever virus vaccine strain Flc-LOM-BErns between cells;a vaccine composition for oral administration comprising same; and a method for serologically differentiating between swine infected outdoors with swine fever virus and swine inoculated with the attenuated swine fever virus live marker vaccine strain. The safety of the attenuated swine fever virus live marker vaccine strain, CSFV BErns attenuated C-P50, according to the present invention has beenenhanced by mitigating side effects, such as abortions/stillbirths in pregnant sows, which are problems of conventional swine fever virus vaccine strains. Also, the vaccine composition for oral administration according to the present invention can be made into bait dosage forms and easily consumed by swine and thus has solved the problem of conventional swine fever virus vaccines being used only as injections and thus difficult to be administered to fierce farmed wild boars and black pigs. Further, the serological differentiation method according to the present invention enables differentiation of the antigens and antibodies of swine infected outdoors with swine fever virus and swine inoculated with the attenuated swine fever virus live marker vaccine strain, CSFV BErns attenuated C-P50, and thus has the excellent effect of differentiating infected from vaccinated animals (DIVA).

Description

technical field [0001] The present invention relates to an attenuated swine fever virus live marker vaccine strain (attenuated swine fever virus live marker vaccine strain) and a vaccine composition for oral administration using the same, more specifically, relates to an existing swine fever virus vaccine strain Flc-LOM-BErns carries out the attenuated classical swine fever virus labeled live vaccine strain (CSFV BErns attenuated C-P50) obtained by intercellular alternate continuous culture, the vaccine composition containing it for oral administration and field infection of classical swine fever virus The serological identification method of pigs and pigs inoculated with the attenuated classical swine fever virus labeled live vaccine strain. Background technique [0002] Classical Swine Fever Virus (CSFV) is an RNA virus with a size of about 40 to 50 nm and belongs to the Flaviviridae family. In addition, it is a virus belonging to the genus Pestivirus and consists of a 12...

Claims

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Application Information

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IPC IPC(8): C12N7/04C12N15/85A61K39/12G01N33/569G01N33/535A61K39/00
Inventor 安东濬任成仁崔世恩赵寅锈郑惠英宋载永金在炤韩圭河玄芳勋金柄汉
Owner 大韩民国农林畜产食品部农林畜产检疫本部
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