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A kind of SNP molecular marker related to pig growth rate and its application

A growth rate and molecular marker technology, applied in the field of molecular genetics, can solve the problem of lack of functional molecular markers, and achieve the effect of shortening the generation interval, increasing the growth rate and low cost.

Active Publication Date: 2021-02-09
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still a lack of molecular markers with clear functions, significant effects, and direct application in breeding.

Method used

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  • A kind of SNP molecular marker related to pig growth rate and its application
  • A kind of SNP molecular marker related to pig growth rate and its application
  • A kind of SNP molecular marker related to pig growth rate and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Expression Analysis of TRPC1 Gene in Example 1 Pig Longissimus Dorsi

[0034] The Chinese local breeds "Tibetan Pig" (collected from the teaching practice ranch of Tibet Agriculture and Animal Husbandry College), "Wujin Pig" (collected from the teaching practice ranch of Tibet Agriculture and Animal Husbandry College) and the imported breed "Darkie Pig" (collected from Anhui Pig Breeding Co., Ltd.) Longissimus dorsi muscle tissue samples at the age of 6 months, using the traditional method of Trizol to extract tissue RNA, and reverse transcribed into cDNA.

[0035] Download the porcine TRPC1 gene mRNA sequence (accession number NM_001145751.1) from NCBI, and use the software PrimerPremier 5.0 to design the following primers:

[0036] Forward primer F: 5'-CATCCAAAGGCAAGGTTA-3'

[0037] Reverse primer R: 5'-AAGTCCGAAAGCCAAGTA-3'

[0038] Use the above primers to carry out real-time fluorescence quantitative PCR amplification on the cDNA of Tibetan pig, Wujin pig and Yor...

Embodiment 2

[0040] Example 2 Screening of pig TRPC1 gene SNP sites

[0041] The Chinese local breeds "Tibetan Pig" (collected from the teaching practice ranch of Tibet Agriculture and Animal Husbandry College), "Wujin Pig" (collected from the teaching practice ranch of Tibet Agriculture and Animal Husbandry College) and the imported breed "Darkie Pig" (collected from Anhui Genomic DNA was extracted from ear tissue samples from Pig Breeding Co., Ltd. using the phenol / chloroform method.

[0042] Download the porcine TRPC1 gene sequence (accession number NC_010455.5) from NCBI, and use the software Primer Premier5.0 to design four pairs of primers for the DNA sequence of the TRPC1 gene downloaded from NCBI. Primer 1 amplifies the 5' of porcine TRPC1 gene Regulatory region -2142bp~-1548bp, the length of the target amplified fragment is 595bp; primer 2 amplifies the 5' regulatory region of porcine TRPC1 gene -1702bp~-942bp, and the length of the target amplified fragment is 761bp; primer 3 amp...

Embodiment 3

[0055] Example 3 Detection of the regulatory activity of the SNP site segment promoter of porcine TRPC1 gene

[0056] Primers were designed according to the sequence of the porcine TRPC1 gene 5' regulatory region, and the primer sequences were:

[0057] Forward primer F: 5'-CTAGAGTTGTGATGGGTCTTC-3';

[0058] Reverse primer R: 5'-GCTCATTGTAAATCTGTGGC-3'

[0059] The primer amplifies the 5' regulatory region of porcine TRPC1 gene -2142bp~-1548bp, which contains two SNP sites, C-1763T and C-1604T. The length of the target amplified fragment is 595bp, and the nucleotide sequence is as shown in SEQ ID NO.1 shown.

[0060] Using pig genomic DNA of different genotypes as a template, the PCR reaction was used to amplify, and the C-1763T and C-1604T sites in the 5' regulatory region of the TRPC1 gene were obtained as C / C and T / T haplotype sequences, respectively. Denote as CC and TT. The amplified fragments were digested, purified, and ligated to construct recombinant pGL3-basic-TR...

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Abstract

The invention provides a SNP molecular marker related to pig growth speed and application thereof. In the present invention, the TRPC1 gene is used as a candidate gene affecting the growth rate of pigs, and the SNP sites related to the growth rate of pigs are screened and identified by sequencing technology, and two SNP sites and genotype effects affecting the growth rate of pigs are obtained. The two SNP sites are respectively located at the 595bp and 539bp of the sequence shown in SEQ ID NO.1, and their allele types are C and T, and the alleles of the two SNP sites are C and C, T It is completely linked with T on the genome, so any of the above SNP sites can be applied to the selection of pig growth rate traits, and it is not limited by the age, sex, and breed of pigs, and can be used for early breeding of pigs. Speed ​​up the breeding process.

Description

technical field [0001] The invention relates to the field of molecular genetics, in particular to a SNP molecular marker related to pig growth speed and the application of the molecular marker in breeding. Background technique [0002] Pork has been occupying more than 60% of my country's total meat consumption for a long time, and it is the main body of animal husbandry production. As people's awareness of environmental protection gradually increases, domestic environmental protection policies are gradually tightened, and the cost of pig farming is also gradually rising; faster growth speed corresponds to high production efficiency, so growth speed is an important target trait in pig genetic improvement. However, the growth rate of pig breeds is regulated by multiple transcription factors and epigenetics, which has always been one of the difficult problems in the field of genetics and breeding. Traditional breeding mainly uses phenotypes to infer genotypes and estimate bre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
Inventor 张浩付玉田小龙张戌园张博张雅文
Owner CHINA AGRI UNIV