A blood sampling device for preparing supernatant stimulated by bovine tuberculosis

Abstract

The present invention firstly provides a blood collection device for bovine tuberculosis stimulation supernatant preparation, which includes an inner cylinder and other commonly used components of the blood collection device, and a mixture of heparin and a cocktail antigen is placed in the inner cylinder of the blood collection device, and the cocktail Antigens include ESAT6, CFP10, FixB and Rv3615c proteins. The invention simplifies the traditional method, and can directly detect after only blood collection and incubation. The entire test process can be carried out in a common laboratory, and each person can save 4 hours for the stimulation supernatant detection of 50 cows. It makes the originally complicated bovine tuberculosis gamma-interferon test very easy to popularize. At the same time, the cocktail antigen used in the blood collection device can eliminate false positive reactions caused by vaccine immunization.

Description

technical field [0001] The invention belongs to the field of animal epidemic detection equipment, and in particular relates to a blood sampling device for preparing supernatant stimulated by bovine tuberculosis. Background technique [0002] Bovine tuberculosis is a chronic consumptive zoonotic disease. It is an animal disease that must be notified according to the International Organization for Animal Health (OIE). It is a second-class animal infectious disease in my country. pose a major threat. According to statistics, about 5% of human tuberculosis is caused by Mycobacterium bovis. Therefore, it is of great public health significance to grasp the current situation of bovine tuberculosis in my country and to eliminate infected cattle through quarantine. The quarantine of bovine tuberculosis can be classified into three categories at present, bacteriological detection, molecular biological detection and immunological detection. The sample collection of bacteriological de...

AI Technical Summary

Problems solved by technology

[0010] It can be seen that the preparation process of the stimulated supernatant used in the bovine tuberculosis gamma interferon ELISA test is cumbersome, the amount of reagent consumables is large, and the workload is heavy. In addition to the time to harvest the supernatant, each person can prepare the stimulated supernatant of 50 cattle. takes 5 or more hours

At the same time, the determination of ELISA results is relatively complicated, and it is difficult to promote it in laboratories with poor conditions, and it cannot rule out false positive reactions caused by vaccine immunization.

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