60Co-gamma ray radiation mutation breeding method for iris sanguinea tissue culture seedlings

A technology for mutation breeding and tissue culture seedlings, which is applied in horticultural methods, botanical equipment and methods, and plant genetic improvement, etc. Stable mutants and other issues, to achieve the effect of increasing the breeding mutation frequency, shortening the breeding cycle, and increasing the mutation frequency

Inactive Publication Date: 2019-05-31
HEILONGJIANG ACAD OF SCI INST OF NATURAL RESOURCES
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AI Technical Summary

Problems solved by technology

[0005] There is a single breeding method for Iris plants, currently there are only conventional breeding techniques such as selfing and distant hybridization, and there is no report on the use of tissue culture seedlings of Iris to induce radiation and produce stable mutants.

Method used

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Experimental program
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Embodiment Construction

[0025] Creek sun tissue culture seedlings 60 Co-γ-ray radiation mutation breeding method, comprising the steps of:

[0026] 1) Selection of tissue cultured seedlings: select the tissue cultured bottle seedlings with excellent growth in the multiplication culture stage as radiation materials;

[0027] 2) 60 Co-gamma radiation treatment: 60 Co-γ-ray radiation treatment doses were 20Gy, 30Gy, 40Gy, 50Gy, respectively, after irradiating different tissue cultured seedlings to obtain different doses of radiation treated tissue cultured seedling groups, recorded as different doses of radiation experimental group;60 Co-γ-ray irradiated seedlings were used as control group;

[0028] 3) Primary selection of mutants of tissue-cultivated seedlings: select the group that survived in different doses of radiation experiment groups after radiation, and record it as the survival experiment group; compare the plant traits of the survival experiment group and the control group, and screen the ...

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Abstract

The invention relates to a 60Co-gamma ray radiation mutation breeding method for iris sanguinea tissue culture seedlings. The method comprises the following steps of 1, tissue culture seedling selection; 2, 60Co-gamma ray radiation treatment; 3, tissue culture seedling mutant primary selection; 4, enrichment subculture of primarily selected mutants; 5, rooting culture of primarily selected mutanttissue culture seedlings; 6, domestication transplanting; 7, field seedling mutant screening; 8, cutting propagation, tissue culture propagation and artificial selection. According to the method, theiris sanguinea tissue culture seedlings are adopted as materials, 60Co-gamma rays are utilized for mutation treatment, the mutagenic effect of different radiation dosages on the iris sanguinea tissueculture seedlings is observed, the proper radiation dosage is determined, and the iris sanguinea tissue culture seedling 60Co-gamma ray radiation mutation breeding technology is set up; by means of the method, rich heritable variations can be created, the breeding period of iris sanguinea is effectively shortened, a simple, convenient and efficient method is provided for new variety breeding, andthe hereditary basis of iris sanguine genetic resources is widened.

Description

technical field [0001] The present invention relates to a kind of Tissue culture seedling 60 Co-γ-ray radiation mutation breeding method. Background technique [0002] Iris sanguinea is a perennial herbaceous flower belonging to the family Iridaceae, with blue-purple and white flowers, black-brown reticular and yellow spots at the base of the outer perianth, and no appendages. It can grow well in wetlands and dry lands. It has strong disease resistance, cold resistance and moisture resistance, and has high ornamental value. It is an excellent material for garden wetland landscaping and creating natural wild interest. The breeding work of Iris plants in my country is progressing slowly, and the research on the breeding technology of Iris is of great significance for enriching the germplasm resources of Iris plants and developing and utilizing wetland plant resources. [0003] Radiation mutagenesis is mainly to treat materials with a certain dose of radiation, so as to cause...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01H4/00
Inventor 曲彦婷韩辉张天姝熊燕张兴斯琴图雅陈菲李黎唐焕伟张洪运
Owner HEILONGJIANG ACAD OF SCI INST OF NATURAL RESOURCES
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