Method for detecting diagnostic marker of follicular thyroid carcinoma using expression level of aminoacyl-trna synthetase-related protein
一种蛋白表达、合成酶的技术,应用在生物测试、测量装置、材料检验品等方向,能够解决特异性低、突变发生率不高、没有用于区分甲状腺滤泡癌和滤泡性腺瘤等问题
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Embodiment 1
[0080] Biotype identification of thyroid tissue proteins
[0081] A total of 2,909 protein classes were identified in follicular adenoma tissue and 2,739 protein classes were identified in follicular thyroid carcinoma tissue respectively, for a total of 4,162 proteins ( figure 1 ) for biological identification. This is unprecedented in academia to date, and is the result of the most extensive protein analysis of thyroid cancer.
Embodiment 2
[0083] Gene Ontology Analysis
[0084] The results of Gene Ontology analysis showed that the functional classification of various proteins related to oxidation / reduction, protein localization, and intracellular transport in the follicular adenoma patient group samples was obvious (List 2), but in the follicular thyroid carcinoma patient group, proteolysis However, the functional classification of proteins involved in the decomposition of macromolecules, RNA processing, and cell cycle is different (Table 1).
[0085] 【List 1】
[0086]
[0087] 【List 2】
[0088]
[0089] Especially among the proteins specifically expressed in the thyroid tissue of follicular carcinoma of the thyroid gland, the increase of the proteins related to the adhesion plaque was more significant than that of the thyroid tissue of follicular adenoma. It was also shown that proteins associated with the spliceosome are specifically increased in follicular thyroid carcinoma.
Embodiment 3
[0091] Analysis of protein expression tendency related to aminoacyl-tRNA synthetase (ARS)
[0092] Among the proteins identified by the biological category using the analysis results, after identifying the proteins associated with ARS, standard-free semi-quantitative analysis was performed based on the peak area. The results showed that 20 ARSs and AIMPs in follicular adenoma tissue and 21 ARS and AIMP in thyroid follicular carcinoma tissue were confirmed, and the quantitative analysis results are shown in the following table (List 3, List 4).
[0093] 【List 3】
[0094]
[0095] 【List 4】
[0096]
[0097] In particular, in follicular carcinoma of the thyroid tissue, AARS (Alanine-tRNA ligase) decreased, AIMP1 (aminoacyl-tRNA synthetase complex-interacting multifunctional protein 1), KARS (lysine-tRNA ligase) and VARS (valine-tRNA ligase) increased ( figure 2 , image 3 ). This study demonstrated for the first time that AIMP1 and KARS were increased in thyroid fol...
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