A kind of preparation and recovery method of Guangdong Cordyceps grass biomass
A technology of protoplasts and Cordyceps guangdong, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., to achieve the effect of promoting the progress of research applications and accelerating the speed of research
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[0025] The preparation of embodiment 1 Guangdong Cordyceps grass protoplast
[0026] (1) Preparation of culture medium and KC buffer:
[0027] YMPT liquid medium: Take 6g of malt extract, 10g of tryptone, 20g of glucose, and 6g of yeast extract, mix them, add deionized water to make up to 1L, sterilize at 121°C for 20min.
[0028] TB 3 Regeneration medium: Take 3g of yeast extract, 3g of acid hydrolyzed casein, 200g of sucrose, 15g of low-melting point agarose, add distilled water to make up to 1L, and sterilize at 121°C for 20min.
[0029] KC buffer: take KCl 13.42g, CaCl 2 1.665g, add deionized water to make up to 300mL, and sterilize at 121°C for 20min.
[0030] (2) Preparation and recovery of Guangdong Cordyceps protoplasts:
[0031] Inoculate the mycelium of Cordyceps guangdong into 200mL YMPT liquid culture medium, 120rpm, 24 ℃ cultivate 12d, obtain the tender mycelium (mycelia) of Cordyceps guangdong( figure 1 ). To prepare an enzyme solution, add 0.15 g of a mix...
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