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A kind of preparation and recovery method of Guangdong Cordyceps grass biomass

A technology of protoplasts and Cordyceps guangdong, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., to achieve the effect of promoting the progress of research applications and accelerating the speed of research

Active Publication Date: 2022-03-04
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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Problems solved by technology

Different fungi have their own enzymatic hydrolysis systems and hydrolysis conditions. At present, there have been studies on the preparation and recovery of protoplasts in the Cordyceps fungi Cordyceps sinensis, Cordyceps militaris, Paecilomyces cicadae, and Cordyceps kaohsiung. However, the protoplasts of Cordyceps guangdong The preparation method has not been established yet, and the method of preparing Cordyceps protoplasts using the mixed enzyme system of lyase and collapse enzyme has not been reported yet

Method used

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  • A kind of preparation and recovery method of Guangdong Cordyceps grass biomass
  • A kind of preparation and recovery method of Guangdong Cordyceps grass biomass
  • A kind of preparation and recovery method of Guangdong Cordyceps grass biomass

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Embodiment 1

[0025] The preparation of embodiment 1 Guangdong Cordyceps grass protoplast

[0026] (1) Preparation of culture medium and KC buffer:

[0027] YMPT liquid medium: Take 6g of malt extract, 10g of tryptone, 20g of glucose, and 6g of yeast extract, mix them, add deionized water to make up to 1L, sterilize at 121°C for 20min.

[0028] TB 3 Regeneration medium: Take 3g of yeast extract, 3g of acid hydrolyzed casein, 200g of sucrose, 15g of low-melting point agarose, add distilled water to make up to 1L, and sterilize at 121°C for 20min.

[0029] KC buffer: take KCl 13.42g, CaCl 2 1.665g, add deionized water to make up to 300mL, and sterilize at 121°C for 20min.

[0030] (2) Preparation and recovery of Guangdong Cordyceps protoplasts:

[0031] Inoculate the mycelium of Cordyceps guangdong into 200mL YMPT liquid culture medium, 120rpm, 24 ℃ cultivate 12d, obtain the tender mycelium (mycelia) of Cordyceps guangdong( figure 1 ). To prepare an enzyme solution, add 0.15 g of a mix...

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Abstract

The invention discloses a preparation and recovery method of Guangdong Cordyceps grass biomass. The preparation method includes the steps of mycelium cultivation, protoplast preparation, protoplast purification, etc., using a mixed enzyme system containing lyase and collapse enzyme to enzymolyze the mycelia of Cordyceps guangdong to obtain the protoplasts of Cordyceps guangdong, and Use TB 3 The recovery medium was used to recover the protoplasts of Cordyceps prairie in Guangdong. The present invention establishes the preparation and recovery system of Guangdong Cordyceps protoplasts for the first time, which is beneficial to the implementation of technologies such as late genetic transformation research or protoplast fusion of Cordyceps cantonese, accelerates the research speed of its genetics and breeding, and helps to promote its in health care. The progress of research and application in the field of food and medicine, benefiting society.

Description

technical field [0001] The invention belongs to the biological field of fungi, and in particular relates to a method for preparing and recovering Guangdong Cordyceps protoplasts. Background technique [0002] Cordyceps guangdongensis is a new species published in 2008, which has been successfully domesticated and established a mature fruiting body cultivation system. Its fruiting body is safe and non-toxic, and was officially approved by the Ministry of Health in 2013 as "new resource food" (now renamed as "new food raw material"). Experiments have found that the fruiting body of Cordyceps guangdong has significant pharmacological effects, including anti-avian influenza virus, anti-fatigue and life extension, treatment of chronic renal failure, treatment of chronic bronchitis, and immune regulation. Previous studies also found that Cordyceps guangdong contains rich metabolites, including polysaccharides, fatty acids, cordycepic acid, amino acids, adenosine, trace elements, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 邓旺秋黄虹李泰辉张成花李挺
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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