Application of nano-gold probe in preparation of early diagnosis reagent for kidney injury

A nano-gold probe, kidney injury technology, applied in the field of diagnostic pharmacology, can solve the problems of high price and inability to be widely used, low resolution and inability to achieve diagnostic effect, etc.

Active Publication Date: 2021-10-01
FUDAN UNIV
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  • Abstract
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Problems solved by technology

However, these methods also have many application limitations
For example, CT, MRI and PET-CT cannot be widely used because of their high price, while ultrasound and X-ray cannot achieve good diagnostic results due to their low resolution.
[0004] All of the above-mentioned deficiencies limit the application of glomerular filtration rate and endogenous markers in the early diagnosis of kidney injury. A Fusion of Optical Visualization

Method used

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  • Application of nano-gold probe in preparation of early diagnosis reagent for kidney injury
  • Application of nano-gold probe in preparation of early diagnosis reagent for kidney injury
  • Application of nano-gold probe in preparation of early diagnosis reagent for kidney injury

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Experimental program
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Effect test

Embodiment 1

[0072] (1) Establish uuo-r animal model

[0073] All experiments were approved by the Animal Care and Use Committee of Shanghai Medical College, Fudan University. The present invention uses male adult balb / c mice (provided by Shanghai Slack Experimental Animal Co., Ltd., Shanghai, China).

[0074] 20 male balb / c mice, weighing about 20, were anesthetized with 2% pentobarbital intraperitoneal injection (50mg / kg) after the mice were weighed, and the mice were fixed on the mouse board in a supine position, and the skin was prepared and sterilized , making an incision on the left side of the abdomen, exposing and separating the left ureter, and using a silk thread to the left. The ureter was double-ligated at the kidney, and the wound was sutured after checking that there was no continuous bleeding in the surgical field of view. After 96 hours, the above operation was repeated and the ligation was removed.

[0075] Tissue collection: Anesthetized with 2% pentobarbital intraperit...

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Abstract

The invention belongs to the field of diagnostic pharmacology, and relates to the use of nano-gold probes in the early diagnosis of kidney damage, especially the use of nano-gold probes in the early diagnosis of kidney damage in diabetic nephropathy. The experimental results of the present invention show that the kidney damage induced under the condition of unilateral ureteral obstruction and release can be detected by the nano gold probe, but cannot be detected by traditional blood biochemical indicators and tissue section methods. Indicating the role of nano-gold probes in the early diagnosis of kidney injury. The nano-gold probe of the present invention detects kidney damage through different particle sizes, and the nano-gold probe of the present invention provides meaningful reference data and support for a new method of diagnosing kidney damage.

Description

technical field [0001] The invention belongs to the technical field of diagnostic pharmacology, and relates to the use of nano-gold probes in the preparation of early diagnostic reagents for kidney damage, especially kidney diseases that affect glomerular filtration pore size. Background technique [0002] Epidemiological studies have shown that there are more than 100,000 patients with end-stage renal disease in the world at present, and the annual growth rate of the number of patients with kidney disease is 10%. 10% of the total number of patients with kidney disease in the world is in China. Moreover, the incidence of some basic diseases such as hypertension and diabetes is increasing year by year, and the number of patients with kidney disease caused by these reasons is also increasing. Kidney disease will not only greatly damage the quality of life of patients, but also cost a lot of treatment. Among people aged 60 and above, the medical consumption of kidney disease pa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K49/04A61K49/22G01N21/17G01N21/33
CPCA61K49/0004A61K49/04A61K49/22G01N21/1702G01N21/33
Inventor 张雪梅彭文潘万马
Owner FUDAN UNIV
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