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Application of dendrobium polysaccharide in preparation of reproductive injury recovery drugs after cancer chemotherapy

A technology of dendrobium polysaccharides and chemotherapy drugs, applied in the application field of dendrobium polysaccharides and drugs for the recovery of reproductive damage after cancer chemotherapy, can solve the problems of lack of research reports on the prevention and treatment of reproductive function damage, and achieve the convenience of large-scale production and low production costs Low, easy-to-handle effect

Inactive Publication Date: 2019-07-09
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, there is a lack of research reports on the prevention and treatment of reproductive function damage caused by radiotherapy and chemotherapy of Dendrobium and its components.

Method used

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  • Application of dendrobium polysaccharide in preparation of reproductive injury recovery drugs after cancer chemotherapy
  • Application of dendrobium polysaccharide in preparation of reproductive injury recovery drugs after cancer chemotherapy
  • Application of dendrobium polysaccharide in preparation of reproductive injury recovery drugs after cancer chemotherapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Extraction and Detection of Dendrobium Polysaccharides

[0054] 1. Extraction of Dendrobium polysaccharides

[0055] Get Dendrobium candidum (Dendrobium candidum is planted in the experimental base of the Institute of Crops of Guangdong Academy of Agricultural Sciences, is identified as Dendrobium candidum through species identification, fresh strips are sampled and then dried and crushed to obtain Dendrobium candidum dry powder) 4g, according to the material of 1:30 (m / V) Add distilled water to the liquid ratio, fully dissolve, extract three times in a 90°C water bath, 120 minutes each time, divide the extract into 7mL centrifuge tubes, freeze and solidify in a -20°C refrigerator, and freeze in a freeze dryer

[0056] Centrifuge at 10000r / min for 6 hours, and concentrate the liquid components in the tube to concentrate the extract. Dissolve and collect the concentrated product with 1-2 mL of distilled water, then add 4 times the volume of absolute ethanol, p...

Embodiment 2

[0065] Changes in the sperm and testis-related parameters of embodiment 2 model mice

[0066] Cisplatin Injection is produced by Jiangsu Hansoh Pharmaceutical Co., Ltd., specification: 6mL: 30mg, National Drug Approval: H20040813.

[0067] 1. Solution preparation

[0068] (1) Preparation of dendrobium polysaccharide solution: take the dendrobium polysaccharide powder prepared in Example 1, dissolve it in distilled water, and prepare a 100 mg / mL dendrobium polysaccharide solution.

[0069] (2) Preparation of HTF solution (human fallopian tube culture fluid)

[0070] Prepare the HTF solution according to the formula in Table 1.

[0071] Table 1 HTF liquid formula

[0072]

[0073] 2. Animal testing

[0074] (1) Blank control group (from day 0 of the experiment, normal saline was injected intraperitoneally, and normal saline was intragastrically administered the next day (after 24 hours) for 7 or 14 consecutive days.)

[0075] (2) Dendrobium polysaccharide group (from day...

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Abstract

The invention discloses an application of dendrobium polysaccharide in the preparation of reproductive injury recovery drugs after cancer chemotherapy. The dendrobium polysaccharide has obvious therapeutic effect and small side effects, which can effectively reduce the damage caused by platinum anticancer drugs to the reproductive system, not only the sperm quantity of mice is significantly increased, the sperm quality is also improved, the antioxidant activity of mouse testicular tissue is also effectively improved, and in mouse testicular tissue, the level of SOD, GPx, GSH and CAT is obviously increased while the level of MDA is obviously decreased. The dendrobium polysaccharide with wide source is simple in extraction and purification method, and the obtained dendrobium polysaccharide is high in sugar content and suitable for large-scale production; the dendrobium polysaccharide has a good prospect in the preparation and clinical application of reproductive injury recovery drugs after cancer chemotherapy.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of dendrobium polysaccharides in the preparation of drugs for the recovery of reproductive damage after cancer chemotherapy. Background technique [0002] In today's society, the incidence and mortality of cancer are increasing year by year. In 2012, the number of cancer diagnoses in my country reached 3.5862 million, and 2.1866 million died of various cancers. In 2015, the number of cancer diagnoses rose to 4.292 million, while the death toll rose to 2.841 million, and the trend of younger age is becoming increasingly prominent. The top ten malignant tumors leading to high mortality in my country are gastric cancer, liver cancer, esophageal cancer, colorectal cancer, leukemia and lymphoma, cervical cancer, nasopharyngeal cancer, and breast cancer. major threat. The current cancer treatment is mainly surgical resection and radiotherapy and chemotherapy. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P15/08
CPCA61K31/715
Inventor 芦春斌张裕明李春梦朱倍倍仇平乐
Owner JINAN UNIVERSITY
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