Preparation method of amauroderma rude polysaccharide F212 and application thereof to prevention and/or treatment of breast cancer

A technique for the preparation of F. chinensis and F212, which is applied to the preparation of F. chinensis polysaccharide F212, and the application field of preventing and/or treating breast cancer, and can solve the problems of difficulty in reaching medicinal levels, mixing, and blurred extract components.

Active Publication Date: 2019-08-02
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Application Information

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On the one hand, the ingredients of this kind of research are vague and mixed...

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  • Preparation method of amauroderma rude polysaccharide F212 and application thereof to prevention and/or treatment of breast cancer
  • Preparation method of amauroderma rude polysaccharide F212 and application thereof to prevention and/or treatment of breast cancer
  • Preparation method of amauroderma rude polysaccharide F212 and application thereof to prevention and/or treatment of breast cancer

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preparation example Construction

[0036] A preparation method of the above-mentioned polysaccharide F212 from Pseudomonas rugosa, comprising:

[0037] (1) Dried, pulverized, and extracted with water, preferably with hot water, to obtain the water extract of F. chinensis, and adding alcohol to the water extract, preferably at a mass percentage concentration It is 95%-100% alcohol, more preferably 95% alcohol by mass percentage, adjusted to 80% alcohol mass percentage concentration, standing at room temperature, preferably standing overnight, and then centrifuging, separating and collecting the precipitate product, to obtain the crude polysaccharide F0 of Fructus Chinensis;

[0038] (2) Purify F0 using DEAE sepharose fast flow, using sodium chloride solution as eluent to carry out gradient elution, the flow rate is preferably 20-40ml / min, and the concentration of sodium chloride solution is preferably 0.1, 0.5, 1, 2Mol / l, 4 components were collected;

[0039] (3) Using breast cancer cells to screen the activit...

Embodiment 1

[0052] Example 1: Preparation of polysaccharide F212

[0053] 1. After drying and crushing 1kg of the fruiting body of False Chrysanthemum rugosa, it is extracted with hot water to obtain a water-extracted concentrated solution of False Ginger. Add 95%-100% alcohol to the concentrated solution and adjust it to 80%. Leave it overnight, then centrifuge and precipitate to separate, collect the precipitate, and obtain the crude polysaccharide F0 of False Chrysanthemum wrinkle cap, with a yield of 1%-4%;

[0054] 2. Purify F0 with DEAE sepharose fast flow, and carry out gradient elution. The eluent is eluted with 0.1, 0.5, 1, and 2Mol / l sodium chloride in turn at a flow rate of 5ml / min, and gradient collection is carried out to obtain 4 components;

[0055] 3. Activity screening experiment: breast cancer cells (4T1 and MDA-MB-231) were used for activity screening of the four components.

[0056] 4. Screen out the most active component F1, purify it with Sephaacyl S-200chromatogra...

Embodiment 2

[0058] Example 2: Composition analysis of the polysaccharide F212

[0059] After testing, the molecular weight of the polysaccharide F212 is MN (number average molecular weight): 4.5852e3g / mol, MW (weight average molecular weight): 5.8659e3g / mol.

[0060] After testing, the polysaccharide F212 monosaccharide composition of A. chinensis is: 4.307% ribose, 0.717% rhamnose, 5.265% arabinose, 4.527% xylose, 30.527% mannose, 37.284% glucose, and 17.374% galactose.

[0061] Gel chromatographic analysis and infrared spectroscopic analysis were carried out on the polysaccharide F212 of A. figure 1 As shown, the spectrogram as figure 2 shown. The attribution of the absorption peaks in the infrared spectrum is shown in Table 1.

[0062] Table 1 Interpretation of the Absorption Peaks of F212 Infrared Spectrum

[0063]

[0064] Interpret the absorption peaks of the infrared spectrum, and see Table 1 for the assignment of each peak. Combined with infrared spectroscopy figure 2 A...

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Abstract

The invention relates to a polysaccharide, in particular to a preparation method and application of an amauroderma rude polysaccharide F212. The amauroderma rude polysaccharide F212 comprises the following monosaccharides in percent by mass: 4.3% of ribose, 0.7% of rhamnose, 5.3% of arabinose, 4.5% of xylose, 30.5% of mannose, 37.3% of glucose and 17.4% of galactose. By the preparation method of the polysaccharide F212, the polysaccharide F212 is obtained by separating and purifying amauroderma rude sporocarps. The polysaccharide F212 is applied to prevention and/or treatment of breast cancer.The invention provides the preparation method and application of the amauroderma rude polysaccharide F212. Researches in the invention show that the amauroderma rude polysaccharide F212 has an anti-tumor property and is safe and non-toxic, especially for the breast cancer, and has a wide application prospect and a good economic value.

Description

technical field [0001] The present invention relates to polysaccharides, in particular to a preparation method of the polysaccharide F212 and its application for preventing and / or treating breast cancer. Background technique [0002] The official journal of the American Cancer Society published the report "Global Cancer Statistics 2018," which assessed the incidence and mortality of 36 cancers in 185 countries. According to the data, the incidence of lung cancer remains high, and among women, breast cancer occupies the top spot. For every 100 new cancer patients in the world, the Chinese account for 21. It is equivalent to that more than 10,000 people are diagnosed with cancer every day in China, and an average of 7 people get cancer every minute. Shocking big data informed the public of the harsh cancer status. Cancer has a high mortality rate and is prone to recurrence and metastasis. Current antitumor drugs generally have the characteristics of large toxic and side ef...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P35/00A23L33/125A23L31/00
CPCA23V2002/00A61K31/715A23L31/00A23L33/125A61P35/00C08B37/0003C08B37/006A23V2200/308A23V2250/208A23V2300/14
Inventor 潘鸿辉吴清平谢意珍雷苏炜蔡超
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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