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A pharmaceutical composition and its application in the preparation of antitumor drugs

An anti-tumor drug and composition technology, applied in the field of biomedicine, can solve the problems of unclear anti-cancer mechanism and action target, and achieve the effect of inhibiting colony formation and improving the inhibitory effect.

Active Publication Date: 2021-12-03
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it has been reported that flubendazole has certain anticancer effects, but its specific anticancer mechanism and target are still unclear.

Method used

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  • A pharmaceutical composition and its application in the preparation of antitumor drugs
  • A pharmaceutical composition and its application in the preparation of antitumor drugs
  • A pharmaceutical composition and its application in the preparation of antitumor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The tumor cell proliferation experiment was carried out by MTT assay, and the effects of flubendazole (Flu) and pentafluorouracil (5-Fu) alone and in combination on cell proliferation were compared. The experimental steps were as follows:

[0028] Spread 3000 cells / well in a 96-well plate. After the cells adhere to the wall, add different drug concentrations of flubendazole or pentafluorouracil single drug or a combination of the two (the solvent is DMSO), and add 20ul after 48 hours of drug action. / well MTT solution (5 mg / ml). After continuing to incubate for 4 hours, the culture was terminated, and the culture supernatant in the well was carefully aspirated and discarded. Add 150ul DMSO to each well to dissolve the crystals. Measure the light absorption value of each well at a wavelength of 490nm with an enzyme-linked immunosorbent monitor, and calculate the combination coefficient. The results are shown in Figure 1 ~ Figure 3 , Figure 1 ~ Figure 3 The unit of d...

Embodiment 2

[0035] Tumor cells can proliferate indefinitely to form cell colonies. Through colony formation experiments, we can understand the effect of drugs on cell proliferation. Spread cells evenly in a 6-well plate at a rate of 1000 cells / well. After the cells adhere to the wall, add the single drug or combination of the two drugs. After 24 hours of action, remove the drug, add fresh medium to continue culturing, and wait for the cells to form colonies visible to the naked eye. , fixed with 4% paraformaldehyde and stained with crystal violet, compared the size and number of colonies in each well, the results are shown in Figure 4 . The results showed that the combination of drugs can more significantly limit the formation of tumor cell colonies.

[0036] The results of Examples 1 and 2 show that the combination of flubendazole and pentafluorouracil can significantly inhibit the proliferation and colony formation of colorectal cancer cells, and reduce the effective drug concentratio...

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Abstract

The invention discloses a pharmaceutical composition and its application in the preparation of antitumor drugs, which consists of pentafluorouracil and flubendazole. In the present invention, pentafluorouracil and flubendazole are used to form a new pharmaceutical composition, and through the synergistic effect of the two, the inhibitory effect on rectal cancer cells is obviously improved, and at the same time, colony formation of colorectal cancer cells can be significantly inhibited.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the application of the combination of flubendazole and pentafluorouracil in the preparation of antitumor drugs. Background technique [0002] Tumor is one of the main causes of human death, and its morbidity and mortality are generally increasing every year. Colorectal cancer is one of the most common digestive tract malignancies worldwide and one of the leading causes of cancer-related deaths. At the same time, with the process of urbanization and the improvement of people's living standards, the incidence of colorectal cancer continues to increase. Although many advances have been made in the research of colorectal cancer, including screening and treatment, the overall survival rate of colorectal cancer patients is still low, and the tumor recurrence rate remains unchanged. Complex tumor pathogenesis, poor tumor treatment effect, high recurrence and metastasis rate, hig...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/513A61K31/4184A61P35/00
CPCA61K31/4184A61K31/513A61P35/00A61K2300/00
Inventor 赵承光林士崇杨乐和左志贵赵海洋周斌胡万乐朱建东李继法崔日
Owner WENZHOU MEDICAL UNIV
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