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Analysis method of volatile metabolic markers in Nicotiana tabacum L. with aroma mutant based on GC-MS characteristics

A technology of metabolic markers and analysis methods, applied in the field of analysis of volatile metabolic markers based on GC-MS characteristic aroma mutant tobacco, can solve problems such as easy oxidation, biological stress effects, inaccurate metabolic profile characterization, etc. To achieve the effect of ensuring accuracy and expanding the scope and field of application

Pending Publication Date: 2019-08-09
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inventors found that the current methods based on GC-MS technology and metabolomics generally have the following problems in the study of volatile metabolites during tobacco growth: because tobacco volatile metabolites are easily oxidized, degraded and rearranged, etc. characteristics, and is greatly affected by the outside world, and the dynamic adsorption method is often used in the in situ collection of plant volatiles, which is likely to cause biological stress effects and cannot reflect the true state of the tobacco plant, resulting in inaccurate characterization of the metabolic profile

Method used

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  • Analysis method of volatile metabolic markers in Nicotiana tabacum L. with aroma mutant based on GC-MS characteristics
  • Analysis method of volatile metabolic markers in Nicotiana tabacum L. with aroma mutant based on GC-MS characteristics
  • Analysis method of volatile metabolic markers in Nicotiana tabacum L. with aroma mutant based on GC-MS characteristics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] 1 Materials and methods

[0063] 1.1 Experimental materials

[0064] The material of this example is the characteristic fragrance mutant material with lemon fragrance and rose fragrance and its control Zhongyan 100. The field test of the tested materials was carried out at the Luozhuang Experimental Station in Zhucheng, Shandong Province. In the budding period of tobacco plants (mid-July), 6 plants with normal growth and consistent growth were selected for each material, and the volatile substances of 2-3 pieces of terminal buds or lateral buds were analyzed by headspace-solid phase microextraction method. Collected in situ in the field.

[0065] 1.2 Instruments and reagents

[0066] Instruments: gas chromatography-mass spectrometer Agilent 7890GC / 5975MSD (Agilent Technologies Co., Ltd., USA); custom-made headspace vials (inertized triangular flasks with SPME sampling ports); freeze dryers (CHRIST, Germany); Solid-phase microextraction device (manual SPME sampler an...

experiment example 1

[0091] The difference between the method for collecting and detecting organic volatiles in flue-cured tobacco plants of this experimental example and that of Example 1 is that the tobacco plants with good growth conditions and no insect damage are selected as the research object, and 2-3 pieces of terminal buds are selected at the beginning of flowering stage. Other steps Same as Example 1, the results showed that 18 volatile components were detected in the three flue-cured tobacco varieties, and β-elemene in the lemon-flavored and rose-flavored flue-cured tobacco varieties was not detected, nor was it detected in the rose-flavored variety The characteristic component of (4R,4aS,6S)-4,4a-dimethyl-6-(prop-1-en-2-yl)-1,2,3,4,4a,5,6,7-octa Hydronaphthalene and farnesene were tested for principal component analysis of the volatile substances, and the results showed that among the three flue-cured tobacco varieties, lemon-flavored flue-cured tobacco and China Tobacco 100 clustered i...

experiment example 2

[0093] The difference between the method for collecting and detecting organic volatiles in flue-cured tobacco plants of this experimental example and that of Example 1 is that the tobacco plants that are well grown in the field without insect damage are taken as the research object, and 2-3 pieces of terminal buds are selected at the budding stage. Take it back to the laboratory and place it in a silanized triangular flask. The other steps are the same as in Example 1. A total of 20 volatile components were detected in the 3 varieties of flue-cured tobacco. At the same time, the rose-flavored flue-cured tobacco variety was not detected in this experimental example. The unique substances in γ-terpinene and (4R,4aS,6S)-4,4a-dimethyl-6-(prop-1-en-2-yl)-1,2,3,4,4a, 5,6,7-octahydronaphthalene, principal component analysis of the detected volatile substances, the results showed that among the three varieties of flue-cured tobacco, lemon-flavored flue-cured tobacco and China Tobacco 1...

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Abstract

The invention provides an analysis method of volatile metabolic markers in Nicotiana tabacum L. with aroma mutants based on GC-MS characteristics. The problems of collection, identification and semi-quantitative detection of unstable and easily degradable active volatile matter can be effectively solved, the spatio-temporal dynamic information of plant organic volatile matter molecules is mastered, a theoretical basis is provided for studying the change, types and mechanisms of plant organic volatile metabolites, and the analysis method of the volatile metabolic markers in the Nicotiana tabacum L. with the aroma mutants based on the GC-MS characteristics is suitable for distinguishing and identifying flue-cured tobacco with the different aroma mutants; and therefore, good practical application value is achieved.

Description

technical field [0001] The invention belongs to the technical field of tobacco metabolomics analysis, and in particular relates to a method for analyzing volatile metabolic markers of tobacco based on GC-MS characteristic aroma mutants. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Tobacco (Nicotiana tabacum L.) is an important economic crop in the world, and aroma is an important indicator for evaluating tobacco. In the sensory evaluation of the 100-point system, aroma alone accounts for 32 points among the six indicators. There are generally problems such as insufficient aroma quantity and single aroma types in tobacco varieties in my country. Tobacco aroma is affect...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 林樱楠曹建敏邱军曹鹏云徐晓玲庞雪莉
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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