Fluorescent probe for detecting mercury ions and preparation method and application of fluorescent probe

Abstract

The invention provides a fluorescent probe for detecting mercury ions and a preparation method and application of the fluorescent probe. The chemical name of the fluorescent probe is 3-(4-(1,3,5,7-tetramethyl-8 propyl)boron fluoride dipyrrol-4-carbonyl)piperazine)-rhodamine B hydrazide. The fluorescent probe takes a boron fluoride dipyrrol fluorescent dye BODIPY unit as an energy donor, a rhodamine unit serves as an energy receptor, by means of bivalent mercury ions, 'on-off' of a spiramide ring is promoted, the purpose that the BODIPY unit and the rhodamine unit are subjected to fluorescenceresonance energy transfer to cause fluorescent red shift is achieved, and then a fluorescence ratio type probe can be used for detecting Hg<2+>; the fluorescent probe takes n-(3-hydroxyphenyl)piperazine and 2-(4-diethylamino)-2-hydroxyl)-benzoic acid as the raw materials, after a reaction is conducted, the raw materials and (1,3,5,7-tetramethyl-8-propoxycarbonyl succinimide)boron fluoride dipyrrolare subjected to a condensation reaction, and the fluorescent probe is obtained. The probe is an FRET-based ratio type fluorescent probe which is high in sensitivity, good in selectivity and capableof detecting the mercury ions in an aqueous phase.

Description

technical field [0001] The invention relates to the technical field of cation detection; in particular, it relates to a fluorescent probe for detecting mercury ions, a preparation method and application thereof. Background technique [0002] Mercury ion (Hg 2+ ) is a very physiologically toxic heavy metal ion, which can be directly enriched in the human body through the skin, digestive tract or respiratory tract, and cause various extremely lethal diseases, such as Minamata disease, which is very harmful. Hg 2+ The mechanism of causing the disease has not been fully studied, and it is widely accepted that Hg 2+ It can combine with certain sulfur-containing groups (such as sulfhydryl) in proteins in the body to form Hg-S complexes, leading to protein inactivation. Important physiological activities in the body are accomplished by proteins, such as nucleoproteins that form the nucleus and affect cell functions; enzyme proteins can promote food digestion, absorption and util...

AI Technical Summary

Problems solved by technology

"Turn-on" and "turn-off" type fluorescent probes both realize the detection of the target through the change of the single fluorescence emission spectrum, so they are easily affected by the light source and the sample environment during the detection process, resulting in accurate detection lower

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