Method for promoting callus of postharvest potato tubers by Candida olive

A technology of olive false silk and potato block, which is applied in the agricultural field, can solve the problems of pesticide residue environment and pollution, and achieve the effects of enhancing disease resistance, health and environmental protection, and promoting postharvest healing

Active Publication Date: 2022-07-19
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for Candida olive to promote postharvest potato tuber callus to solve the problem that chemical drugs will cause pesticide residues and environmental pollution in promoting potato tuber callus

Method used

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  • Method for promoting callus of postharvest potato tubers by Candida olive
  • Method for promoting callus of postharvest potato tubers by Candida olive
  • Method for promoting callus of postharvest potato tubers by Candida olive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, potato tuber is processed according to the following steps:

[0026] A. Inoculate Candida olive on the slanted surface of nutritional yeast glucose solid medium (NYDA) for initial activation, the activation temperature is 28 ° C, and the activation time is 2 days, and the activated Candida olive is prepared with sterile water. yeast suspension;

[0027] B. Inoculate the yeast suspension prepared in step A into the nutritional yeast glucose liquid medium (NYDB), and cultivate for 24 hours at a temperature of 28 °C and a rotation speed of 220 r / min; re-inoculate the cultured yeast into the nutritional yeast Continue to re-cultivate in yeast glucose liquid medium (NYDB) at a temperature of 28 °C and a rotation speed of 220 r / min to obtain the target yeast suspension, and adjust the final concentration of yeast in the target yeast suspension to 1 × 10 9 cells / mL;

[0028] C. Rinse the potato tubers with tap water first, then soak and disinfect with sodium hy...

Embodiment 2

[0041] Embodiment 2, potato tuber is processed according to the following steps:

[0042] A. Inoculate Candida olive on the slanted surface of nutritional yeast glucose solid medium (NYDA) for initial activation, the activation temperature is 26 ° C, and the activation time is 3 days, and the activated Candida olive is prepared with sterile water. yeast suspension;

[0043] B. Inoculate the yeast suspension prepared in step A into the nutritional yeast glucose liquid medium (NYDB), and cultivate for 24 hours at a temperature of 28 °C and a rotation speed of 220 r / min; re-inoculate the cultured yeast into the nutritional yeast Continue to re-cultivate in yeast glucose liquid medium (NYDB) at a temperature of 28 °C and a rotation speed of 220 r / min to obtain the target yeast suspension, and adjust the final concentration of yeast in the target yeast suspension to 1 × 10 8 cells / mL;

[0044] C. Rinse the potato tubers with tap water first, then soak and disinfect with sodium hy...

Embodiment 3

[0046] Embodiment 3, potato tuber is processed according to the following steps:

[0047] A. Inoculate Candida olive on the slanted surface of nutritional yeast glucose solid medium (NYDA) for initial activation, the activation temperature is 30 ° C, and the activation time is 2 days, and the activated Candida olive is prepared with sterile water. yeast suspension;

[0048] B. Inoculate the yeast suspension prepared in step A into the nutritional yeast glucose liquid medium (NYDB), and cultivate for 24 hours at a temperature of 28 °C and a rotation speed of 220 r / min; re-inoculate the cultured yeast into the nutritional yeast Continue to re-cultivate in yeast glucose liquid medium (NYDB) at a temperature of 28 °C and a rotation speed of 220 r / min to obtain the target yeast suspension, and adjust the final concentration of yeast in the target yeast suspension to 1 × 10 6 cells / mL;

[0049] C. Rinse the potato tubers with tap water first, then soak and disinfect with sodium hy...

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Abstract

The invention discloses a method for promoting the callus of postharvest potato tubers by Candida olive, which belongs to the field of agriculture and solves the problem of pesticide residues and environmental pollution caused by chemical drugs promoting the callus of potato tubers. The present invention includes the following steps: inoculating Candida olive on the inclined surface of the nutritional yeast glucose solid medium for activation, and then preparing the yeast suspension with sterile water; inoculating the yeast suspension into the nutritional yeast glucose liquid medium Carry out two cultivations; rinse the harvested potato tubers, soak and disinfect with sodium hypochlorite, rinse with deionized water and air dry; spray the target yeast suspension evenly on the surface of the potato tuber wound, and put it into a perforated fresh-keeping bag after natural air-drying in the healing process. The present invention adopts non-polluting Candida olive to promote the callus of postharvest potato tubers, and Candida olive can not only have biological control effects on postharvest diseases, but also can enhance the disease resistance of potato tubers.

Description

technical field [0001] The invention belongs to the field of agriculture, in particular to a method for promoting postharvest potato tuber callus by Candida olive. Background technique [0002] Potato (Solanum tuberosum. L) is the fourth largest food crop in the world. It is widely grown all over the world and has a huge impact on world food security and economic development. Potato tubers will inevitably have wounds during harvesting and transportation, and these wounds will become the main channels for harmful microorganisms to infect potato tubers. Potato tubers can form callus through self-calling, which provides the main protection against the infection of pathogens. However, the natural callus of potato will be affected by the external environment, and the temperature, humidity and oxygen are not suitable for callus. At the concentration, the callus of potato was further inhibited. Although some chemicals have been reported to promote the callus of potato tubers, thi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16A23B7/155A23B7/157
CPCA23B7/155A23B7/157
Inventor 郑晓渊毕阳姜红王斌王调兰
Owner GANSU AGRI UNIV
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