Drug-loaded nano-composite, preparation method thereof and application of nano-composite

A drug-loaded nano-composite technology, applied in drug combinations, pharmaceutical formulations, anti-tumor drugs, etc., can solve the problem of solubility, the mechanism of metabolic toxicity of tumors is not fully researched, the drug damages normal organs, and increases the metabolic burden. and other problems to achieve the effect of improving load stability, avoiding metabolic burden and stable release

Inactive Publication Date: 2019-08-16
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the field of tumor treatment technology, the toxic and side effects of small-molecule chemotherapy drugs in clinical practice greatly limit the use of chemotherapy drugs, and small-molecule chemotherapy drugs often release too quickly or burst during the administration process, which increases the The metabolic burden of the drug also exacerbates the damage of drugs to normal organs; although the immunotherapy based on fullerene and its derivatives can inhibit tumor growth by regulating the tumor microenvironment, its efficacy is limited, and there are solubility problems, metabolic toxicity, etc. The mechanism of action on tumors has not yet been fully elucidated

Method used

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  • Drug-loaded nano-composite, preparation method thereof and application of nano-composite
  • Drug-loaded nano-composite, preparation method thereof and application of nano-composite
  • Drug-loaded nano-composite, preparation method thereof and application of nano-composite

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Experimental program
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Effect test

Embodiment 1

[0087] This embodiment provides a drug-loaded nanocomposite, the preparation method of which specifically includes the following steps:

[0088] (1) 20mg PEG-co-PLGA was dissolved in 4mL dichloromethane to obtain solution A;

[0089] (2) 0.4mg of Gd@C 82 (OH) 22 Dissolve in 400 μL of secondary water to obtain solution B;

[0090] (3) Mix solution B obtained in step (2) with 0.4 mg of doxorubicin hydrochloride, and stir for 24 hours at room temperature in the dark to obtain solution C;

[0091] (4) Mix solution A obtained in step (1) with solution C obtained in step (3) under magnetic stirring, and ultrasonically emulsify for 2 minutes at a power of 50 W with an ultrasonic breaker to obtain colostrum;

[0092] (5) Add 6mL emulsifier to the colostrum that step (4) obtains, obtain double emulsion after ultrasonic breaker 10min under the power of 100W with ultrasonic breaker; , and then centrifuged at a high speed to obtain the drug-loaded nanocomposite.

Embodiment 2

[0094] This embodiment provides a drug-loaded nanocomposite, the preparation method of which specifically includes the following steps:

[0095] (1) 30mg PEG-co-PLGA was dissolved in 4mL dichloromethane to obtain solution A;

[0096] (2) 0.6mg of Gd@C 82 (OH) 22 Dissolve in 400 μL of secondary water to obtain solution B;

[0097] (3) Mix solution B obtained in step (2) with 0.8 mg of doxorubicin hydrochloride, and stir for 24 hours at room temperature in the dark to obtain solution C;

[0098] (4) Mix solution A obtained in step (1) with solution C obtained in step (3) under magnetic stirring, and ultrasonically emulsify for 1 min at a power of 75W with an ultrasonic breaker to obtain colostrum;

[0099] (5) Add 9mL emulsifier to the colostrum that step (4) obtains, obtain double emulsion after ultrasonic breaker 7min under the power of 200W with ultrasonic breaker; , and then centrifuged at a high speed to obtain the drug-loaded nanocomposite.

Embodiment 3

[0101] This embodiment provides a drug-loaded nanocomposite, the preparation method of which specifically includes the following steps:

[0102] (1) 50mg PEG-co-PLGA was dissolved in 4mL dichloromethane to obtain solution A;

[0103] (2) 1 mg of Gd@C 82 (OH) 22 Dissolve in 400 μL of secondary water to obtain solution B;

[0104] (3) Mix solution B obtained in step (2) with 3 mg of doxorubicin hydrochloride, and stir for 24 hours at room temperature in the dark to obtain solution C;

[0105] (4) Mix solution A obtained in step (1) with solution C obtained in step (3) under magnetic stirring, and ultrasonically emulsify for 5 minutes at a power of 100 W with an ultrasonic breaker to obtain colostrum;

[0106] (5) Add 15mL emulsifier to the colostrum that step (4) obtains, and obtain double emulsion after ultrasonic breaking under the power of 500W with an ultrasonic breaker for 10min; the double emulsion obtains a dispersion after rotary steaming under reduced pressure at roo...

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Abstract

The invention provides a drug-loaded nano-composite, a preparation method thereof and an application of the nano-composite. The drug-loaded nano-composite comprises a core-shell structure and anti-tumor drugs, the core-shell structure takes metal-containing fullerol as a core and takes an amphiphilic polymer as a shell, and the anti-tumor drugs are loaded on the surface of the metal-containing fullerol core. The core-shell structure of the drug-loaded nano-composite can achieve massive steady load and slow release functions for the drugs, the metal-containing fullerol has anti-tumor activity and the function of relieving toxic and side effects of chemotherapeutic drugs on bodies, so that a drug carrier and the anti-tumor drugs are cooperatively matched with each other, immunological therapy and chemotherapy for tumors can be combined, and tumor therapy effects are greatly improved. The drug-loaded nano-composite has good long cycle effects and tumor targeting properties, and the preparation method is simple and good in stability.

Description

technical field [0001] The invention belongs to the technical field of drug-loaded materials, and in particular relates to a drug-loaded nanocomposite and its preparation method and application. Background technique [0002] At present, tumor has become the number one killer that threatens human health. The basic principle of traditional tumor treatment methods (such as chemotherapy, radiotherapy, etc.) is to kill tumor cells. It leads to high tumor recurrence rate and unsatisfactory clinical treatment effect. In recent years, with the rapid development of biological technology and the discovery of a large number of drug targets, tumor immunotherapy has become a research hotspot. A variety of tumor immunotherapy drugs, such as Ipilimumab, Nivolumab, Pembrolizumab, etc., have been approved by the FDA for clinical use However, there are still problems that cannot be ignored in the use of small molecule and antibody drugs, such as limited drug efficacy, easy degradation, and u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/14A61K47/04A61K45/00A61K31/704A61P35/00
CPCA61K9/143A61K31/704A61K45/00A61P35/00
Inventor 陈春英唐敬龙刘颖周会鸽
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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