Vibrio alginolyticus bacteriophage, bacteriophage composition and application of vibrio alginolyticus bacteriophage, bacteriophage composition

A technology of vibrio alginolyticus and phage, which is applied in the direction of phage, virus/phage, application, etc. It can solve the problems affecting the treatment effect and resistance mutation, achieve long-term bactericidal effect, avoid anti-phage mutation, and host specificity strong effect

Active Publication Date: 2019-08-16
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, phage therapy also has certain limitations. For example, pathogenic bacteria can easily

Method used

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  • Vibrio alginolyticus bacteriophage, bacteriophage composition and application of vibrio alginolyticus bacteriophage, bacteriophage composition
  • Vibrio alginolyticus bacteriophage, bacteriophage composition and application of vibrio alginolyticus bacteriophage, bacteriophage composition
  • Vibrio alginolyticus bacteriophage, bacteriophage composition and application of vibrio alginolyticus bacteriophage, bacteriophage composition

Examples

Experimental program
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Effect test

Example Embodiment

[0060] Example 1 Isolation of Vibrio alginolyticus phage ValB1 MD

[0061] A sample of offshore sea water was taken, filtered through a 0.22 μm sterile filter membrane, and added to the Vibrio alginolyticus VIB 283 T bacterial solution in the exponential growth phase at a volume ratio of 10%, and cultured with shaking at 28°C. Samples were taken on 1d, 3d, 5d, and 7d. After the samples were filtered and sterilized by a 0.22μm sterile filter, they were diluted with SM Buffer for different concentration gradients, and then 1ml of the diluted filtrate and 1ml of the log phase Vibrio alginolyticusVIB 283 T bacterial solution Mix and incubate with shaking at 28°C for 30min, then pour the melted and pre-cooled RO semi-solid at about 48°C, mix and pour on the RO solid plate, shake it horizontally until the semi-solid solidifies. Incubate the semi-solid plate in a constant temperature incubator at 28°C overnight. If plaque appears, it is a preliminary isolated phage.

Example Embodiment

[0062] Example 2 Purification of Vibrio alginolyticus phage ValB1 MD

[0063] The plaque obtained in Example 1 was soaked in SM Buffer overnight, and then the soaking solution was filtered through a 0.22 μm sterile filter and then diluted with SM Buffer gradient. The double-layer plate was made according to the method described in Example 1, and the double-layer plate was obtained. The evenly dispersed plaques on the plate. Repeat the above operation 3-5 times until the size and shape of the plaque on the double-layer plate obtained last time are consistent. The purified phage double-layer plate see figure 1 .

Example Embodiment

[0064] Example 3 Isolation of Vibrio alginolyticus phage ValB1 HC

[0065] The specific implementation method is the same as that described in Example 1.

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Abstract

The invention provides a vibrio alginolyticus bacteriophage, a bacteriophage composition and an application of the vibrio alginolyticus bacteriophage and the bacteriophage composition and belongs to the technical of microbial control. Two new strains of the vibrio alginolyticus bacteriophage are obtained by screening, and the new strains have strong host specificity and strong cracking and killingeffect on vibrio alginolyticus; meanwhile, the mixed use of the new strains can effectively prevent the vibrio alginolyticus from producing resistant mutation, so as to achieve the ideal pathogen removal effect, and the important application value in the prevention and control of vibrio alginolyticus infectious diseases in marine aquaculture animals is achieved.

Description

technical field [0001] The invention belongs to the technical field of microbial control, and in particular relates to Vibrio alginolyticus phages and phage compositions and applications thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Vibrio alginolyticus is a common opportunistic pathogenic bacteria in aquaculture fishery. It can infect seawater fish and freshwater fish. It manifests as extensive skin damage and bacteremia in fish. In severe cases, it can develop muscle and multiple organ edema and further lead to the death of fish. my country is a large aquaculture country, and the infection of Vibrio alginolyticus causes huge economic losses to the aquacul...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K35/76A61P31/04A01N63/00A01P1/00C12R1/92
CPCA01N63/00A61K35/76A61P31/04C12N7/00C12N2795/10121C12N2795/10131C12N2795/10132
Inventor 张永雨李成成
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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