Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method of 20(r)-ginsenoside rg3 and its application in the preparation of heat stress-induced spermatogenic damage medicine

A technology of ginsenoside and heat stress, which is applied in the field of medicine for the development of natural drug activity, to achieve the effect of remarkable efficacy, less dosage, and low-cost acquisition

Active Publication Date: 2020-02-07
JILIN YATAI PHARM CO LTD
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] With the increasing of global warming, a series of problems caused by heat stress are becoming more and more serious, but so far, the protective effect of ginsenoside Rg3 on spermatogenesis damage caused by heat stress in the scrotum has not been reported. The present invention proposes And it proves that 20(R)-Rg3 can be used to treat and prevent spermatogenic damage caused by environmental heat stress, which is closely related to people's life and has strong practicability

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of 20(r)-ginsenoside rg3 and its application in the preparation of heat stress-induced spermatogenic damage medicine
  • Preparation method of 20(r)-ginsenoside rg3 and its application in the preparation of heat stress-induced spermatogenic damage medicine
  • Preparation method of 20(r)-ginsenoside rg3 and its application in the preparation of heat stress-induced spermatogenic damage medicine

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0027] Experimental Example 1: Preparation and isolation of R-Rg3 using ginsenoside Rd as a substrate

[0028] 1.1 Single factor investigation experiment

[0029] 1.1.2 The effect of acid concentration on the reaction

[0030] Accurately weigh 20 mg of ginsenoside Rd powder and dissolve in 1 mL of formic acid solutions with concentrations of 0.05%, 0.1%, and 0.2%, respectively, and react at 100° C. for 3 h. After the reaction, the sample was centrifuged to obtain a precipitate and the supernatant was concentrated. Part of the precipitate was removed and the methanol was settled in a 10 mL volumetric flask for HPLC analysis. The content of rare ginsenoside Rg3 in the precipitate was calculated according to the peak area. The results are shown in Table 1. It can be seen from the results that the content of Rg3 is low at 0.05%, and there is incomplete hydrolysis of Rd, while the generation of rare ginsenoside Rg3 is at a maximum at 0.1%, and the ratio of by-product Rk1 / Rg5 is lo...

experiment example 2

[0044] Experimental example 2: Protective effect of R and S configuration ginsenoside Rg3 on mouse Leydig cells TM3

[0045] The experimental method refers to the literature (Zhao Jian, et al. Establishment of oxidative stress model of TM3 cells induced by hydrogen peroxide, China Public Health, 2015, 31, 312-314). The specific experimental scheme is omitted.

[0046] As shown in Table 4, the experimental results show that the two configurations of Rg3 were applied to TM3 cells at three doses for 24 hours, and the dosage of hydrogen peroxide was 600 μM. configuration, especially the effect of 6μM in the high-dose group was more obvious.

[0047] According to literature reports and test results of the present invention (Hee-Won Park, et al., J Ginseng Res2013,37,457-467; Jin Yan, et al., Plant Foods Hum Nutr, 2015,70:141-145; Lee, et al. ., J.Ginseng Res.2012,36,102-106), the ratio of S configuration to R configuration Rg3 in red ginseng is about 2:1~3:1, as attached figure ...

experiment example 3

[0054] Experimental Example 3: The protective effect of 20(R)-ginsenoside Rg3 on the damage of germ cells induced by heat stress in the scrotum

[0055] 3.1 Materials and methods

[0056] 3.1.1 Materials, reagents and instruments

[0057] 20(R)-ginsenoside Rg3 (hereinafter referred to as Rg3), purity > 98.5% (HPLC); sodium pentobarbital (C 11 h 17 N 2 NaO 3 ), purity>99%.

[0058]Malondialdehyde (MDA), reduced glutathione (GSH) and superoxide dismutase (SOD) kits were purchased from Nanjing Jiancheng Bioengineering Institute; testosterone (Testosterone) kits were purchased from American R&D Corporation . BP211D electronic balance, Germany Sartorius company; DK-98-1 type electric heating constant temperature water bath, Tianjin Test Instrument Co., Ltd.; HC-2517 high-speed centrifuge, Anhui Zhongke Zhongjia Company; Epoch ultra-micro microplate spectrophotometer , American Boten Instrument Co., Ltd.; FSH-2A adjustable high-speed homogenizer, Jintan Huacheng Hailong Exper...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of medicines, and specifically discloses application of 20(R)-ginsenoside Rg3 (R-Rg3, C42H72O13) in preparing medicine for treating or prevent spermatogenesis damagecaused by heat stress (HS). The main R-Rg3 is mainly obtained by performing acid hydrolysis reaction on diol type saponin Rd to prepare a composition containing the R-Rg3, and then performing traditional preparative separation to obtain a target product with high purity. In vivo and in vitro experimental study shows that the R-Rg3 has prominent effects in treating the spermatogenesis damage caused by the HS, problems of fertility disfunction and the like caused by the HS can be effectively avoided, no toxic and side effects exist, the R-Rg3 is a safe and effective, and stable medicine which is simple in preparation technology and can treat the HS, the medicine is suitable for industrial production, and the promotion is easy. According to the invention, a new medicine source for treating and preventing the spermatogenesis damage caused by the environmental HS and series of problems thereof is provided.

Description

Technical field: [0001] The invention belongs to the medical technical field of active development of natural medicines, relates to a medicine or health care product for treating spermatogenic damage, in particular to a preparation method of a natural compound-20(R) ginsenoside Rg3 and its use in the preparation of treatment or prevention environment New application of heat stress-induced spermatogenesis damage drugs and health products. Background technique [0002] Heat stress (heat stress, HS) refers to the stress response of general organisms under adverse environmental conditions such as high temperature. The incidence of infertility is gradually increasing. However, people pay more attention to the impact of environmental pollutants and infection on male reproduction, but the impact of environmental temperature rise and lifestyle changes on male reproductive development and function has not been fully paid attention to. Spermatogenesis is a temperature-dependent proc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/704A61P15/08A23L33/105
CPCA23L33/105A23V2002/00A61K31/704A61P15/08A23V2200/30A23V2250/2124
Inventor 李伟李新殿王梓李有宝任珅姜爽刘伟冷晶
Owner JILIN YATAI PHARM CO LTD