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Method for preparing monascus pigment by using yellow serofluid as raw material

A technology of monascus pigment and yellow pulp water, which is applied in the field of preparation of red yeast rice pigment, can solve the problems of insufficient utilization of nutrients in yellow pulp water, environmental pollution of yellow pulp water discharge, etc., to solve resource waste and environmental pollution, and achieve significant economic benefits The effect of high benefit and high production efficiency

Pending Publication Date: 2019-08-30
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for preparing monascus pigment with yellow pulp water as raw material, so as to solve technical problems such as insufficient utilization of yellow pulp water nutrients, environmental pollution caused by yellow pulp water discharge, etc.

Method used

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  • Method for preparing monascus pigment by using yellow serofluid as raw material
  • Method for preparing monascus pigment by using yellow serofluid as raw material
  • Method for preparing monascus pigment by using yellow serofluid as raw material

Examples

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Embodiment 1

[0026] This embodiment illustrates the method of preparing red yeast rice pigment by fermenting with yellow pulp water as a raw material and only adding glucose as a carbon source. The method is as follows:

[0027] Add 80g / L, 100g / L, 120g / L glucose respectively to the yellow pulp water to make the fermentation medium, sterilize at 121°C for 20 minutes, cool to room temperature naturally, then, under aseptic conditions, press 10% (V / The inoculum amount of V) was inserted into the Monascus seed liquid, and cultivated in the dark for 7 days at 30° C. on a constant temperature shaker at 150 rpm. Then the pigment fermentation liquid was centrifuged at 10000rpm for 5 minutes, and the supernatant was diluted to an appropriate multiple, so that the absorbance was finally between 0.2-0.6, and the absorbance was measured at 420nm and 500nm wavelengths to measure its extracellular color value; 70% alcohol to the volume of the original fermentation broth, extract in a water bath at 60°C...

Embodiment 2

[0032] This example illustrates the method of preparing red yeast pigment by fermenting with yellow pulp water as a raw material and only adding soluble starch as a carbon source. The method is as follows:

[0033] Add 80g / L, 100g / L, and 120g / L soluble starch to the yellow pulp water to make fermentation medium, sterilize at 121°C for 20 minutes, cool naturally to room temperature, and then, under aseptic conditions, press 10% (V The inoculation amount of / V) was inserted into the Monascus seed liquid, and cultivated in the dark for 7 days at 30° C. on a constant temperature shaker at 150 rpm. Then the pigment fermentation liquid was centrifuged at 10000rpm for 5 minutes, and the supernatant was diluted to an appropriate multiple, so that the absorbance was finally between 0.2-0.6, and the absorbance was measured at 420nm and 500nm wavelengths to measure its extracellular color value; 70% alcohol to the volume of the original fermentation broth, extract in a water bath at 60°C...

Embodiment 3

[0038] This embodiment illustrates the method of preparing red yeast rice pigment by fermenting with yellow pulp water as a raw material and only adding old rice flour as a carbon source. The method is as follows:

[0039] Add 80g / L, 100g / L, and 120g / L aged rice flour to the yellow pulp water to make fermentation medium. The aged rice flour comes from Jinjian rice industry indica rice purchased from the market and stored naturally for more than 5 years, crushed and passed through a 60-mesh standard sieve Gained, cultivated based on 121°C sterilization for 20 minutes, naturally cooled to room temperature, then under aseptic conditions, insert the Monascus seed liquid at an inoculum size of 10% (V / V), at 30°C, a constant temperature of 150rpm Incubate in the dark on a shaker for 7 days. Then the pigment fermentation liquid was centrifuged at 10000rpm for 5 minutes, and the supernatant was diluted to an appropriate multiple, so that the absorbance was finally between 0.2-0.6, and...

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Abstract

The invention provides a method for preparing a monascus pigment by using yellow serofluid as a raw material. The method uses the yellow serofluid as a raw material, and different carbon-source substances are respectively added to prepare fermentation liquor so as to prepare the monascus pigment. When glucose is added as a carbon source, the fermentation measures that the maximum yield of the pigment transformed from the glucose is 862.8+ / -13.6 U / g; when soluble starch is added as a carbon source, the fermentation measures that the maximum yield of the pigment transformed from the soluble starch is 739.4+ / -7.48 U / g; and when old rice flour is added as a carbon source, the fermentation measures that the maximum yield of the pigment transformed from the old rice flour is 1066+ / -18.75 U / g. The method takes the yellow serofluid as a raw material and adds different carbon sources to produce the monascus pigment, well solves the problems of resource waste and environmental pollution caused by discharging the yellow serofluid as waste, prolongs the industrial chain, and turns waste into valuable. The method is simple in preparation process, low in cost, good in waste utilization, economical and environmentally friendly, high in production efficiency and remarkable in economic benefit.

Description

technical field [0001] The invention relates to the technical field of production of monascus pigments, in particular to a method for preparing monascus pigments by using yellow pulp water as a raw material. Background technique [0002] Monascus pigment is a polyketide secondary metabolite produced by Monascus through solid-state fermentation or liquid submerged fermentation. In the past two thousand years, it has been widely used in food coloring, and now it also plays a huge role in the food industry. For example, Monascus pigment is used in sausages, fermented bean curd, traditional brewed rice wine, seafood, and meat products. They are widely used. At the same time, monascus pigments are also considered to be able to partially replace nitrates and nitrites in Europe for the color enhancement and storage of meat products, such as the color enhancement and preservation of sausages and hams. In addition to being widely used as a food colorant, monascus pigments also have ...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12R1/645
CPCC12P1/02C12R2001/645C12N1/145
Inventor 刘俊罗运川柴雪莹吴井艳赵会赵雯郭亭林亲录
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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