Boletus calopus polysaccharide, and preparation method and application thereof

A technology of boletus and boletus sprouts, which is applied in the field of bolete polysaccharide and its preparation, and can solve the problems of fine structure, immune activity and antitumor activity that have not been reported.

Active Publication Date: 2019-09-06
西充星河生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no reports on the fine structure, immune activity and ...

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  • Boletus calopus polysaccharide, and preparation method and application thereof
  • Boletus calopus polysaccharide, and preparation method and application thereof
  • Boletus calopus polysaccharide, and preparation method and application thereof

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Embodiment Construction

[0049] Unless otherwise defined, all scientific and technical terms used in the present invention have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention pertains.

[0050] The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0051] 1. Structural analysis of the polysaccharide BRS-X from Boletus americana

[0052] 1.1 Extraction, separation and purification of polysaccharides from Boletus americana

[0053] 1.1.1 Extraction of crude polysaccharides from Boletus mesicola by hot water extraction

[0054] Powder the dried fruiting body of Boletus melica, and put it in a water bath at 100°C for 6 hours according to the liquid-to-material ratio of 3:1, repeat 3 times, collect the supernatant and concentrate. The crude polysaccharide was precipitated with three volumes of absolute ethanol, and the protein in the crud...

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Abstract

The invention discloses a Boletus calopus polysaccharide, and a preparation method and an application thereof. The polysaccharide is obtained through water extraction, alcohol precipitation, protein removal, ion exchange column chromatography and dialysis concentration of Boletus calopus sporophores, and is heteropolysaccharide composed of alpha-D-galactose and beta-D-glucose, wherein a molar ratio of the alpha-D-galactose residue to the beta-D-glucose residue is 5:1. The polysaccharide has a significant activity, and has a significant inhibition activity to multiple tumor cells.

Description

technical field [0001] The invention belongs to the technical field of fungal polysaccharide applications, and in particular relates to a polysaccharide from Boletus americana and its preparation method and application. Background technique [0002] Polysaccharides are natural biological macromolecules that are linked by more than 10 monosaccharides through glycosidic bonds. At present, studies have found that polysaccharides have a variety of physiological effects, such as immune regulation, anti-tumor, anti-virus, anti-oxidation, hypoglycemic and so on. Moreover, the two-dimensional NMR spectrum ( 1 H- 1 The rapid development of technologies such as H NMR, HSQC, HMBC, etc.), gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography (HLPC), X-ray diffraction, and scanning electron microscopy have largely promoted the Structural studies. [0003] Boletus reticulates Schaeff. belongs to Agaricaceae, Boletaceae, and Boletus genus, and is edible a...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P35/00A61P37/04A23L33/125
CPCA23V2002/00A61K31/715A23L33/125A61P35/00A61P37/04C08B37/006A23V2200/324A23V2200/308A23V2250/51A23V2250/208
Inventor 侯怡铃丁祥粟思源
Owner 西充星河生物科技有限公司
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