Boletus calopus polysaccharide, and preparation method and application thereof
A technology of boletus and boletus sprouts, which is applied in the field of bolete polysaccharide and its preparation, and can solve the problems of fine structure, immune activity and antitumor activity that have not been reported.
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[0049] Unless otherwise defined, all scientific and technical terms used in the present invention have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention pertains.
[0050] The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.
[0051] 1. Structural analysis of the polysaccharide BRS-X from Boletus americana
[0052] 1.1 Extraction, separation and purification of polysaccharides from Boletus americana
[0053] 1.1.1 Extraction of crude polysaccharides from Boletus mesicola by hot water extraction
[0054] Powder the dried fruiting body of Boletus melica, and put it in a water bath at 100°C for 6 hours according to the liquid-to-material ratio of 3:1, repeat 3 times, collect the supernatant and concentrate. The crude polysaccharide was precipitated with three volumes of absolute ethanol, and the protein in the crud...
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