A kind of preparation method of cytotoxic T cell
A cytotoxic and cellular technology, applied in the field of preparation of cytotoxic T cells, can solve the problems of low yield and low purity
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[0044] CTL cell culture
[0045] 1. Verify that the suction tube of the plasma bag is sealed. Wipe the pipette and scissors with two different cleanroom wipes soaked in 70% ethanol.
[0046] 2. Hold the suction tube with tweezers, cut the front end of the tube and control the flow rate with tweezers to transfer the plasma into a 50ml test tube. Record the plasma volume transferred.
[0047] 3. Centrifuge the tube (400g, 4°C) for 30 minutes to remove platelets.
[0048] 4. Pour the supernatant liquid into a new 50ml test tube.
[0049] 5. Put the test tube into a constant temperature water tank (56°C) and incubate for 30 minutes.
[0050] 6. Centrifuge tubes (500g, 4°C) for 60 minutes to remove precipitated fibrin.
[0051] 7. Pour the upper liquid (autologous serum) into a new 50ml test tube. Label the date of preparation and refrigerate (4°C).
[0052] 8. Take a new plasma bag to confirm that the seal is intact, and shake the plasma evenly.
[0053] 9. Wipe the suctio...
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