65 results about "Cell harvest" patented technology

The present disclosure is directed to devices, arrays, kits and methods for detecting biomolecules in a tissue section (such as a fresh or archival sample, tissue microarray, or cells harvested by an LCM procedure) or other substantially two-dimensional sample (such as an electrophoretic gel or cDNA microarray) by creating "carbon copies" of the biomolecules eluted from the sample and visualizing the biomolecules on the copies using one or more detector molecules (e.g., antibodies or DNA probes) having specific affinity for the biomolecules of interest. Specific methods are provided for identifying the pattern of biomolecules (e.g., proteins and nucleic acids) in the samples. Other specific methods are provided for the identification and analysis of proteins and other biological molecules produced by cells and / or tissue, especially human cells and / or tissue. The disclosure also provides a plurality of differentially prepared and / or processed membranes that can be used in described methods, and which permit the identification and analysis of biomolecules.

The main objects of the present invention, which relates to regenerative medical treatments, are to enable (i) storage and conveyance of harvested or cultured cells without contamination occuring (ii) simple injection of the cells into a living body. To achieve these objects, cells harvested from a living body, or cells obtained by culturing harvested cells, are stored in a syringe-type storage vessel and subsequently transplanted into a living body. It is preferable that at least a part of the storage vessel inner wall in contact with the cells is formed from a cell non-adhesive material. Besides enabling cells in the vessel to take in the oxygen they require to survive, the present invention also enables cells quick and easy transplantation of cells into a living body without a cell detachment process, because cells are prevented from adhering to the inside of the vessel. Further, it is preferable that a stored tissue regeneration composition contains cell culture microcarriers floating in a fluidity medium, and that the cell culture microcarriers are composed of a bioabsorbable material and have cells adhering to their surfaces. Using this kind of tissue regeneration composition, a regenerative treatment can be carried out satisfactorily by simply and quickly transplanting cells from the syringe-type cell storage vessel into a living body without intricate scaffold-related procedures being required.

A tissue engineering cartilage construction method using bone matrix gelatin which comprises, using cartilage cell for isolated culture or base material stem cell for isolated culture to evoke cartilage cell i.e. seed cell, fetching New Zealand rabbit or human embryon os longum and metaphysic trabecular bone to construct bone matrix gel BMG, inoculating the seed cells harvested through isolated culture onto cortex bone or cancellous bone BMG for extracorporal culture.

A method and apparatus for cell harvest of production scale quantities of cell cultures using single use components comprising a flexible membrane mounted on a rigid frame and is supported within a multiple use rigid centrifuge bowl, such single use components including a core with an increased diameter and an internal truncated cone shape in order to permit the system to maintain a sufficiently high angular velocity to create a settling velocity suited to efficiently processing highly concentrated cell culture streams. Features which minimize feed turbidity, and others which permit the continuous or semi-continuous discharge of cell concentrate, increase the overall production rate over the rate which can be achieved using current intermittent processing methods for large cell culture volumes. Injection of a diluent during the cell concentrate removal process permits more complete removal of viscous cell concentrates.

The invention relates to the biological cell technology field, particularly a method for separating and culturing miniature swine bone marrow-derived endothelial progenitor cells. The conventional EPCs culture method has the disadvantages of complexity and greatly reduced cell harvest rate by using immunomagnetic bead separation. The method in the invention comprises the steps of directly using myelomonocyte to conduct culture in vitro to obtain EPCs, adding growth factors in culture solution, planting in a definite density in a culture flask / vessel or a glass sheet enveloped by fibronectin, and conducing appropriate culture to obtain EPC. The method with high repeatability simplifies the steps of the conventional culture method, effectively avoids unnecessary cell loss caused by the conventional separation and culture method, increases the pick-up rate of EPCs, and saves a large amount of funds. The method of the invention also prepares for clinical application in future, and can reduce the usage of bone marrow and provide a technology platform for curing traumatic or ischemic diseases by autotransfusion.

This invention relates to methods for monitoring and controlling bioprocesses. Specifically, it describes using quasi-real-time analytical and numerical techniques to analyze optical loss measurements calibrated to indicate cell viability, whereby it is possible to reveal process changes and / or process events such as feeding or induction. Additionally, the present invention makes it possible to accurately estimate the onset of a decrease in cell viability and / or a suitable time for cell harvesting for a cell culture growth process. Pattern recognition methods for identifying specific process events such as batch feeding, cell infection, and product precipitation are also described.

A method for the identification of a nucleic acid molecule differentially expressed in an in vitro model of a biological system, comprising the steps of: (1) harvesting cells from the model system at predetermined time points; (2) obtaining total RNA from the cells harvested at each time point; (3) preparing cDNA from the total RNA from each time point to provide a plurality of pools of cDNA; (4) performing a suppression subtractive hybridization (SSH) on the cDNA pools from each time point sequentially so as to progressively amplify cDNAs derived from nucleic acid molecules differentially expressed from one time period to the next.

The present invention relates to compositions and methods for promoting tissue regeneration, preferably neural tissue regeneration. Compositions of the invention include (i) certain diphenyl sulfides, diphenyl sulfoxides, diphenyl sulfones, and sulfide, sulfoxide and sulfones of dibenzothiophene and thioxanthene, as well as various analogues and derivatives of these compounds; (ii) one or more cells harvested from an animal or organism subsequent to the administration of a composition comprising a compound of (i); or (iii) any combination of (i) and (ii). The invention can be useful in treating decreases in neuronal function, for example from injury or disease.

Described herein are novel rapid and reliable methods of detection of extracellular vesicles and quantifying extracellular vesicle concentrations and absolute number from various sources, including raw cell harvest. The methods described herein comprise detection of intrinsic fluorescence of extracellular vesicles in biological samples. Extracellular vesicles analyzed by the methods of this application have a stereotypical elution profile distinct from known contaminants. The methods described herein are a significant improvement over the state of the art and fulfills an unmet need in the field of extracellular vesicle manufacturing and quality control.

The invention discloses a centrifugal settling-type bioreactor for continuous culture of plant cells. The centrifugal settling-type bioreactor comprises a main body which is in a U-shaped structure on the whole; a liquid inlet is also formed in one side of a liquid lifting region; a cell harvest opening is formed in the bottom position of a liquid dropping part; a liquid lifting part is provided with a gas nozzle at the bottom position of the main body; a seal funnel and a spiral pipe are arranged on the liquid dropping part from top to bottom; the top part of the seal funnel and the inner wall of the liquid dropping part of the main body are in fit seal; the bottom part of the seal funnel is connected to the inlet of the spiral pipe; a gravity settling pipe is also connected to the bottom part of the spiral pipe; and the other side of the gravity settling pipe is connected with a waste liquid collector. Separation of cells and a culture solution is achieved by the gravity settling pipe, which is connected with the bottom of the spiral pipe, in the bioreactor; the cell intercept efficiency can be obviously improved by adding the spiral pipe to the reactor, so that continuous perfusion culture of the plant cells is achieved; and the culture efficiency of the plant cells is improved.

The present invention provides improved devices for biopsy, aspiration, stem cell acquisition, and methods of using the same. The devices balance aspiration with concurrent infusion to manage changes in pressure at the site of biopsy. The present invention can be adapted for any biopsy, aspiration, or cell harvest procedure, including adipose tissue aspiration and bone marrow aspiration (BMA). In particular, the present invention limits patient pain, prevents blood contamination, and increases cell mobilization, such as improved stem cell yields with intraosseous (10) pharmacological mobilization of stem cells during a BMA procedure, and with improved stem cell yields using pharmacological mobilization of stem cells from fat. The pharmacological mobilization of cells allows the harvest of cells from a biopsy many fold larger than existing methods.

Devices and methods for cell harvesting are disclosed. More particularly this invention relates to devices and methods for enabling the formation of a dispersion of cells from tissue for medical or research use. This invention provides a rapid method for cell isolation thereby reducing the time and costs associated with the production of autologous cells where required, for example, for tissue engineering in the operating theater or in research. Alternately this invention may be used to separate a cell dispersion containing cells of varying sizes or types from a predigested tissue dispersion. Also a kit is disclosed which provides the basic components of the device and instructions on how to accomplish the use of the device.

Provided are a cell analyzer, and a cell analysis method. A cell analyzer 1 includes a measurement device 2 for detecting information of each cell from a measurement specimen containing cells harvested from an epithelial tissue, and a data processing device 3 for determining appropriateness of the cell harvesting of parabasal cells and acquiring information related to canceration of the cell based on the information detected by the measurement device 2. The data processing device 3 displays on a display section a dialogue showing “cell harvesting inappropriate” when determined that the harvesting of parabasal cells is inappropriate.

The invention discloses an electron microscope quantitative detection method for virus particles by taking nanoparticles as a reference substance. The method comprises the following steps: mixing a cell harvesting liquid containing the virus particles with a DMEM culture medium containing the nanoparticles with the diameter of 20-200nm; then carrying out ultra-high-speed centrifugal treatment, andcollecting the virus particles; re-suspending the collected virus particles into the DMEM culture medium, and then adding 3-5% of agar with the same volume as the DMEM culture medium; after the sample is coagulated into blocks, dehydrating and embedding the blocks, finally cutting the blocks into ultrathin slices with the size of 0.1 mu m as a sample, observing the number of the virus particles and the number of the nanoparticles with the area of 1369 mu m < 2 > under an electron microscope, and calculating the concentration of the nanoparticles in the electron microscope sample according tothe observed number of the virus particles and the number of the nanoparticles; and further calculating the concentration of the nanoparticles in the reference substance, and finally calculating the concentration of the virus particles in cell harvesting liquid. According to the method, errors existing in a traditional detection method can be effectively avoided.

The invention provides an adherent culture method of neural stem cells. The method includes the steps of conducting primary culture on forebrain histocytes to obtain a neural stem cell sphere suspension; digesting neural stem cell spheres into suspension neural stem cells, inoculating the suspension neural stem cells into a petri dish wrapped by human fibronectin for culture, and conducting continuous cell culture after the cells grow to a certain integration degree; using digestive enzymes to digest the cells during follow-up continuous cell culture, after the cells shrink, blowing down the cells and then conducting centrifugation to obtain cell sediments for continuous cell culture. The adherent culture method of the neural stem cells has the advantages that monolayer adherent culture of the cells is achieved in a limited culture area, the cell harvest yield per unit area is clear, and the cell quantity after harvest can be ensured; moreover, the cells after the adherent culture are easy to digest, damage to the cells is reduced, and the viability of the obtained cells is high; the used human fibronectin for facilitating the adherent culture of the neural stem cells is low in price and capable of being utilized over and over again, other reagents are all common, and the cost of the unit cell quantity is low.

The invention discloses a method for mass production for a recombinant adenovirus for a novel coronavirus gene vaccine. The method comprises the following steps of carrying out cell proliferation; inoculating cells harvested through proliferation into a packed bed bioreactor for culture; carrying out virus infection on the cells in the packed bed bioreactor and harvesting a culture solution containing a recombinant adenovirus; filtering and concentrating a recombinant adenovirus collection fluid; adding broad-spectrum nuclease into the recombinant adenovirus collection fluid and carrying out nuclease treatment; and separating and purifying the recombinant adenovirus collection fluid subjected to nuclease treatment by using an anion exchange column chromatography and obtaining the purifiedrecombinant adenovirus. Infection and proliferation of the recombinant adenovirus are carried out by adopting the packed bed bioreactor, so that the efficiency is high and the method is more suitablefor the requirements of a GMP production environment. Separation and purification are carried out by adopting full-automatic column chromatography, so that more economical and efficient large-scale industrial GMP production of recombinant adenovirus preparation products is achieved, and the novel coronavirus gene vaccine is used for preventing infection and transmission of a novel coronavirus.

The invention relates to the technical field of biological tissue engineering, and discloses a lung cancer organoid culture solution and a culture reagent combination and a culture method thereof, and the lung cancer organoid culture solution comprises a complete medium and an autogenous condition medium; the complete culture medium comprises a conditioned culture medium, a basic culture medium, compound antibiotics and growth factors; the autogenous condition culture medium is obtained by culturing fiber cells extracted from lung cancer tissues by using a tumor-associated fibroblast culture medium. All the components of the culture solution have a synergistic effect, so that the formation and growth of lung cancer organoid cells are accelerated, and the culture speed is increased while the cell harvesting number is increased; and the in-vitro cultured organoid is more bionic, can be suitable for personalized culture of the lung cancer organoid, and can maintain the physical structure and pathological characteristics of the primary tissue of each patient.

The invention relates to the field of separation culture of dental pulp stem cells, and discloses a separation culture method of the dental pulp stem cells. The method comprises the following steps: crushing a tooth to expose dental pulp tissues, cutting the dental pulp tissues into pieces, collecting into a centrifuge tube, and using collagenase type I and neutral protease to decompose the dental pulp tissues; adding a complete culture solution to finish the decomposition, centrifuging to remove supernatant, then resuspending cells by using the complete culture solution, and filtering to obtain primary cell suspension liquid; adopting 1*104 / cm<2> as primary cell planting density; two days later, changing the liquid for the first time, then changing the liquid for once every three days, culturing for 9-19 days, wherein about 100 clone cells are formed by each primary cell, and carrying out cell harvesting and passage; planting passage cells according to the planting density of 1*4000 / cm<2>, and carrying out cell harvesting and passage again when the cells reach 70-90% of convergence degree. The separation culture method increases the separation culture efficiency and yield of the dental pulp stem cells and reduces the production cost by regulating the separation culture method of the dental pulp stem cells and all parameters in the concrete operation.

The invention provides a human umbilical cord blood NK cell culture system and a preparation method, and belongs to the technical field of immune cell therapy. The human umbilical cord blood NK cell culture system in the invention comprises human hepatitis B immune globulin, human serum albumin, a serum-free medium, an activating factor and autologous plasma. Human umbilical cord blood NK cells are obtained through the steps of culture bottle coating, umbilical cord blood gradient centrifugation preparation of umbilical cord blood mononuclear cells and autologous plasma, inoculation, liquid changing, bagging, cell harvesting and the like. According to the invention, the human hepatitis B immune globulin and the human serum albumin are creatively combined for coating a culture bottle; pure factors IL2 and IL15 are combined for use; the clinical use risk is reduced; furthermore, the culture period of the preparation method in the invention is 12 days; the cost is reduced; and the prepared NK cells are good in amplification efficiency, high in purity, large in quantity and capable of meeting clinical requirements.

The invention relates to the technical field of separation culture of endothelial progenitor cells of umbilical blood, particularly a separation culture method for endothelial progenitor cells of umbilical blood and a kit of the method. The method comprises the following steps: carrying out adherent culture on a mononuclear cell of umbilical blood in advance; and carrying out cell culture on non-adherent cells, wherein a culture medium for cell culture is a full culture medium containing fetuin. Compared to cells obtained by conventional methods, the endothelial progenitor cells of umbilical blood cultured by the method provided by the invention are relatively uniform in shape, relatively high in convergence degree, relatively great in cell harvest yield, relatively fast in cell proliferation and relatively high in antigen presentation rate on cell surface.

The invention belongs to the biotechnical field of microalgae and relates to a method for sorting microalgae at high throughput. The method specifically comprises the following steps: (1) culturing microalgae: (2) adding chemicals to selectively hydrophobize algae cells; (3) collecting the algae in an air floating method; and (4) detecting the content of target contents of microalgae cells harvested each period of time. The method provided by the invention has the advantages and positive effects that (1) the method is simple to operate and low in cost; 2, the method is suitable for sorting and harvesting various economical microalgae; 3, the microalgae cells with high content of targeted products can be quickly obtained in batches; and 4, the method is mild in operating condition and hardly damages cells.

Described herein are novel rapid and reliable methods of detection of extracellular vesicles and quantifying extracellular vesicle concentrations and absolute number from various sources, including raw cell harvest. The methods described herein comprise detection of light scattering of extracellular vesicles in biological samples. Extracellular vesicles analyzed by the methods of this application have a stereotypical elution profile distinct from known contaminants. The methods described herein are a significant improvement over the state of the art and fulfills an unmet need in the field of extracellular vesicle manufacturing and quality control.