38 results about "Cell harvest" patented technology

The invention provides a preparation method of a cell for expressing a chimeric antigen receptor. The preparation method comprises the following steps: (1) enabling the cell to be in contact with an activating agent for activation; (2) contacting the cell with a nucleic acid molecule for coding the CAR, the nucleic acid molecule for coding the CAR being on a non-viral vector, and obtaining the cell containing the nucleic acid molecule; and (3) harvesting cells. Wherein the step (3) is not later than 72 hours after the step (1) is started. Compared with cells prepared by other similar methods, the cells in the step (3) show a higher CAR positive rate, a higher proportion of undifferentiated cell phenotypes, a lower proportion of depleted cells and a stronger amplification ability.

The present invention relates to a method for rapidly preparing chimeric antigen receptor T cells (CAR-T), comprising the steps of: 1) contacting T cells or a cell population comprising the T cells with a stimulating agent to activate the T cells to obtain activated T cells or a cell population comprising the activated T cells; 2) contacting the activated T cell or a cell population comprising activated T cells with a nucleic acid molecule encoding a chimeric antigen receptor (CAR), thereby obtaining a CAR-T cell expressing the CAR; and (3) harvesting the CAR-T cells, culturing for 18-24 hours, harvesting after the culture is finished, and freezing or not freezing. According to the method, the total preparation time of the CAR-T cells is greatly shortened, and meanwhile, the amplification and tumor killing capabilities of the CAR-T cells are remarkably improved.

The present invention relates to a method and platform for generating CAR-T cells and processing biological cells within a single functionally closed container. The method involves introducing host cells into a sealed container with multiple sterile channels, performing genetic modifications, and maintaining the cells under controlled conditions monitored by real-time feedback until a desired fraction of genetically modified cells is obtained. The container also supports target cell separation using antibody or aptamer functionalized buoyant microbubbles, facilitating the separation of target cells from non-target cells during centrifugation. Waste separation and cell harvesting are performed using pneumatic pressure, enabling high purity cell recovery. The platform includes modules for cell enrichment, sequestration, and isolation, enabling high recovery and high purity of therapeutic cell populations. This integrated closed system approach ensures sterile conditions, efficient processing, and high quality cell products for advanced therapeutic applications.

The invention discloses a full-automatic cell harvesting system and a harvesting control method.The harvesting system comprises a centrifugal bin used for executing centrifugal action, and the centrifugal bin is provided with a liquid inlet channel communicated to the position close to the top of the side wall of a bin body and a liquid outlet channel connected to the bottom wall of the bin body; the liquid adding unit is connected with the liquid inlet channel and comprises a sample branch, a cleaning branch, a resuspension branch and a liquid adding pump; the recovery unit is connected with the liquid outlet channel and comprises a finished product branch, a waste liquid branch and a recovery pump; and the visual detection module is triggered by a pulse signal to carry out image acquisition at a preset frequency, and the pulse signal adjusts the triggering frequency according to the rotating speed of the centrifugal bin. Through cooperation of the centrifugal bin, the liquid adding unit and the recycling unit, full-automatic harvesting of cells is achieved, and the high-throughput cell harvesting requirement can be met.

A method for producing and applying cellular materials from human cells for non-therapeutic applications is provided. The method comprises the steps of obtaining human cells, cultivating the human cells in a controlled environment, harvesting the cultivated human cells, processing the cultivated human cells to produce cellular materials and applying the cellular materials to a human subject for non-therapeutic applications. Other example embodiments are described herein. In certain embodiments, the method offers an environmentally sustainable solution that provides human cellular materials having improved safety, bioactivity, and batch consistency for human use.

1. Name of the design product: bioreactor. 2. Use of the design product: the product is an integrated bioreactor, which can complete cell culture (primary culture), expansion culture, cell culture from tissues, medium replacement and cell harvesting. 3. Design points of the design product: in shape. 4. Picture or photo best indicating the design points: front view.

The invention relates to a cell processing method, comprising the following steps: S1: filling a centrifugal cup with a cleaning liquid: the centrifugal cup is rotated to maintain a centrifugal state, and the cleaning liquid is passed into the centrifugal cup. After the cleaning liquid fills the centrifugal cup, the cleaning liquid is stopped from being pumped in; S2: continuous flow concentration: a cell sample liquid is pumped into the centrifugal cup, and under the action of centrifugal force, the cells adhere to the wall of the centrifugal cup, and the supernatant is discharged from the centrifugal cup; S3: cleaning the cell sample: the cleaning liquid is pumped into the centrifugal cup, and after the cleaning liquid fills the centrifugal cup, a set volume of the cleaning liquid is continuously pumped in and a centrifugal supernatant of the same volume is synchronously pumped out, and the process is maintained for a certain period of time; S4: breaking up the cell sample: the centrifugal state of the centrifugal cup is stopped, and the centrifugal cup is periodically rotated forward and reversely to break up the clumped cells in the centrifugal cup into single suspended cells; S5: cell resuspending: a cell freezing liquid is passed into the centrifugal cup, and the centrifugal cup is mixed by rotating forward and reversely to mix the cell suspension; S6: collecting cells.

This utility model discloses a cell harvester with an automatic sample introduction mechanism, including a chamber and test tubes. A centrifugation mechanism assembly is installed at the top of the chamber, and an automatic sample introduction component is installed at the top of the chamber, directly above the centrifugation mechanism assembly. A sample tube rack is installed at the top of the chamber, and placement slots are evenly distributed at the top of the sample tube rack. Test tubes are placed inside the placement slots. A rotating disk is rotatably connected inside the placement slots, and an arc-shaped groove is evenly distributed around the top of the rotating disk. Multiple sets of clamping rods are installed at the top of the rotating disk, and a connecting shaft adapted to the arc-shaped groove is fixedly installed at the bottom of the clamping rods. A cavity is opened inside the sample tube rack. In this utility model, by pulling the pulling rod, the limiting block is disengaged from the limiting slot, and the adjusting block can be slid. The rotating disk is driven by rack and pinion transmission, so that multiple sets of clamping rods move synchronously, flexibly adapting to test tubes of different sizes and increasing the convenience of use.

The invention provides a method for efficiently separating and culturing mesenchymal stem cells. The method comprises the steps of separating adipose-derived mesenchymal stem cells, culturing the cells and the like. In the steps of adipose tissue treatment and cell separation, a specific digestive enzyme mixed solution is used for digestion treatment, so that the adipose tissue is fully digested, the cell damage is reduced to the greatest extent, the separation of adipose cells is promoted to the greatest extent, and the yield of the cells is remarkably improved. For cell culture, a serum-free culture medium is innovatively adopted. The formula of the culture medium is elaborately blended, a DMEM-F12 culture medium is used as a matrix, and platelet lysate, human serum albumin, recombinant insulin, EGF, bFGF, special oligopeptide and other components in a specific proportion are added. The components have a synergistic effect, so that the potential risk caused by serum is avoided, a suitable growth environment is provided for cells, the growth and proliferation of the cells are promoted, and the undifferentiated state of the cells is maintained.

The invention particularly relates to the technical field of stem cell preparation and tissue engineering, and discloses a placenta mesenchymal stem cell multi-source extraction system based on an integrated treatment platform. Comprising a multi-region synchronous pretreatment module, a zoning adherent culture module, a differentiated cell harvesting module, a cell characteristic collection module, a functional cell division module and a whole-process closed-loop control module, the multi-region synchronous pretreatment module adopts an integrated design, and the zoning adherent culture module realizes centralized culture through an independent marking region; the differentiation cell harvesting module is used for carrying out merging amplification; the cell characteristic acquisition module is used for acquiring cell characteristic data; the functional cell division module is used for realizing cell type and function judgment; the whole-process closed-loop control module is used for realizing whole-process quality tracing; through the integrated design, the cost is reduced, the cell quality stability is ensured, and the whole-process quality tracing is realized.

The utility model discloses automatic production equipment for cell drugs. The automatic production equipment comprises a process operation cavity and a cell factory incubator, the cell factory incubator is communicated with the process operation cavity; the process operation cavity is provided with a cell harvesting area and a cell inoculation liquid changing area; a centrifugal bottle pipetting and cover opening and closing assembly, a centrifugal assembly, a subpackaging assembly and a first transfer mechanism are arranged in the cell harvesting area; the first transfer mechanism is used for transferring the centrifugal bottle between the centrifugal bottle pipetting and cover opening and closing assembly and the centrifugal assembly; a cell factory pipetting and cover opening and closing assembly and a second transfer mechanism are arranged in the cell inoculation liquid changing area; the second transfer mechanism is used for transferring the cell factory among the cell factory pipetting and cover opening and closing assembly, the cell factory incubator and the centrifugal bottle pipetting and cover opening and closing assembly. By means of the arrangement, the function of full-process automation is achieved, and the purposes of reducing manual intervention and improving operation efficiency and sterility are achieved.

The utility model relates to cell medical field discloses a kind of for cell liquid supplement and cell suspension collection device, including collection bottle, filter screen, sealing ring and injection assembly, the filter screen is slidably connected in the collection bottle top, the handle is fixedly connected in the filter screen inside, the sealing ring is slidably connected in the collection bottle outside, the collection bottle top and the sealing ring inside are all fixedly connected with four magnets, the injection assembly is installed in the sealing ring top, the positioning assembly is installed in the injection assembly outside, the injection assembly includes pipeline, and the pipeline output end is fixedly connected in the sealing ring top.In the utility model, by pulling slide bar, piston compression spring one is driven, simultaneously under the cooperation of two check valves, after supplement liquid, cell suspension is inhaled into shell from pipeline left end, then slide bar is pushed, and it will be transported to collection bottle along pipeline right side, and liquid supplement or cell harvesting can be realized quickly, and it is transported to collection bottle, and the equipment structure is simple, easy to operate, and low in operating cost.

A three-dimensional porous growth surface (carriers) and cell culture devices including the same are provided. The carriers are made by multiple layers of netting or mesh, especially large dimension and area of fabrics that are capable to form a column-type fixed bed by rolling the layers or other shape of fixed bed by stacking or randomly disposed packing the carriers to form a packed-bed for cell culture the layers together, thereby sealing the surrounding of the multilayer nettings or meshes to reduce particle generation during cell harvest, or ease of separation by filtration due to larger wall dimension on the nettings or meshes than cells. The fixed bed make by large dimension of the growth surface can easily to manufacture a fixed bed simply by rolling the multiple layers of sheets, which can reduce the manufacture cost and also facilitate mass production of carriers for fixed bed bioreactors.

1. Name of the design product: bioreactor. 2. Use of the design product: the product is an integrated bioreactor, which can complete cell culture (primary culture), expansion culture, cell culture from tissues, medium replacement and cell harvesting. 3. Design points of the design product: in shape. 4. Picture or photo best indicating the design points: front view.

The present application relates to the technical field of cell culture and cell harvesting, and in particular to a lysis powder and its application in lysis of gelatin-based microcarriers. The present application comprises a lysis enzyme module, an EDTA chelation module and a cell protection module, which can realize rapid lysis of gelatin-based microcarriers and release of cells, improve the proportion of live cells in the harvested cells, and maintain the ability of the harvested cells to adhere, recover and proliferate after being reseeded, thereby being suitable for cell engineering, tissue engineering, cell culture meat and related large-scale production scenarios.

Provided is a cell harvesting device. The cell harvesting apparatus comprises: an agitator adapted to receive a bioreactor; and a connector adapted to couple the agitator to the bioreactor.

The invention provides a method for preparing cells expressing a chimeric antigen receptor from cryopreserved cells. The method comprises the following steps: (1) resuscitating the cryopreserved cells; (2) activating the cells by contacting the cells with an activating agent; (3) contacting the cell with a CAR-encoding nucleic acid molecule, the CAR-encoding nucleic acid molecule being on a non-viral vector, to introduce the nucleic acid molecule into the cell; and (4) harvesting cells. According to the invention, the high-quality CAR-T cell is successfully prepared from the cryopreserved PBMC by using an electroporation process, and compared with the CAR-T prepared from the fresh PBMC, the CAR-T cell has the same performance, which proves that the immune cell therapy can be realized through the cryopreservation of the PBMC.

The present invention relates to a rapid preparation method for a chimeric antigen receptor (CAR) T cell (CAR-T cell), comprising the steps of: 1) enabling a T cell or a cell population containing the T cell to come into contact with a stimulating agent to activate the T cell, so as to obtain an activated T cell or a cell population containing the activated T cell; 2) enabling the activated T cell or the cell population containing the activated T cell to come into contact with a nucleic acid molecule encoding a CAR, so as to obtain a CAR-T cell expressing the CAR; and 3) harvesting the CAR-T cell, culturing same for 18-24 hours, harvesting same after the culture, and performing or not performing cryopreservation. The method greatly shortens the total preparation duration of a CAR-T cell, and significantly improves the CAR-T cell proliferation and tumor killing capabilities.

The utility model discloses a full-automatic cell harvesting instrument which comprises a frame, a liquid injection mechanism, a liquid suction mechanism, a centrifugal mechanism and an oscillating mechanism, the liquid injection mechanism, the liquid suction mechanism, the centrifugal mechanism and the oscillating mechanism are located in the frame, the frame comprises a cross beam and a workbench, the cross beam is located above the workbench, and the liquid injection mechanism and the liquid suction mechanism are fixed on the cross beam. The centrifugal mechanism is fixed to the workbench and located below the workbench, the liquid injection needle translation assembly and the liquid injection needle lifting assembly horizontally move on the cross beam, the liquid injection needle lifting assembly vertically moves relative to the liquid injection needle translation assembly, the oscillating mechanism is located below the workbench, and the centrifugal mechanism comprises a plurality of centrifugal test tube racks. The vibration rod drives the centrifugal test tube rack to vibrate through the rotation of the eccentric ball seat. The liquid injection mechanism and the liquid suction mechanism are fixed to the cross beam, the centrifugal mechanism, the oscillation mechanism and the supporting mechanism are fixed to the lower portion of the workbench, cooperative work of multiple mechanisms is achieved, an integrated working platform is achieved, and cell chromosome collection work is completed.

The invention relates to a method for preparing a comestible nutrient composition comprising the steps of (i) cultivating proliferating non-human animal cells of interest in vitro until the cell count of said cells has multiplied by at least 2-fold or more; (ii) harvesting the cells to provide cell-derived material; and (iii) preserving the cell-derived material from the cells harvested in step (ii) from spoiling. Furthermore, the invention refers to a comestible nutrient composition obtainable from such method and use thereof for providing a well-defined nutrient composition.

Disclosed herein is a cell harvesting instrument suitable for concentrating and / or washing cells from a source suspension of cells, the instrument comprising: a housing for accommodating mechanical elements comprising at least one fluid pump, at least one valve; and a treatment kit removably inserted into the housing, the kit comprising a substantially flat frame having or supporting a plurality of sealed fluid paths, the fluid paths being arranged in a substantially plane and such that fluid in the paths does not contact the mechanical element, where at least a portion of the fluid paths comprises a flexible tube, the flexible tube being configured to receive fluid from the fluid paths. The outer surface of the flexible tube may be manipulated by the or each fluid pump to provide fluid flow in one or more of the paths, and / or by the or each valve to constrain fluid flow in one or more of the paths. In an embodiment, the kit also includes a fluid handling reservoir and a filter adapted to separate cells from the fluid in the pathway. Transfer mechanisms for moving and weighing fluid processing reservoirs are also disclosed.

The present invention provides improved devices for biopsy, aspiration, stem cell acquisition, and methods of using the same. The devices balance aspiration with concurrent infusion to manage changes in pressure at the site of biopsy. The present invention can be adapted for any biopsy, aspiration, or cell harvest procedure, including adipose tissue aspiration and bone marrow aspiration (BMA). In particular, the present invention limits patient pain, prevents blood contamination, and increases cell mobilization, such as improved stem cell yields with intraosseous (10) pharmacological mobilization of stem cells during a BMA procedure, and with improved stem cell yields using pharmacological mobilization of stem cells from fat. The pharmacological mobilization of cells allows the harvest of cells from a biopsy many fold larger than existing methods.

The present invention relates to the technical field of CHO cell culture, and specifically relates to a CHO cell additive and its application. The additive specifically includes: asparagine 0.5 - 3.0 g / L, leucine 0.5 - 3.0 g / L, isoleucine 0.5 - 3.0 g / L, valine 0.1 - 1.0 g / L, phenylalanine 0.1 - 1.0 g / L, and metal framework nanoparticles 0.1 - 10 mg / L. By using the additive, during fed-batch culture, the rate at which cells uptake nutrients is more balanced. During the overall culture process, certain key nutrients such as amino acids and vitamins can be maintained until cell harvest, enabling better maintenance of cell viability, and thus increasing the protein yield at cell harvest.

The invention relates to a method and platform for producing CAR-T cells and processing biological cells within a single, functionally closed container. The method involves introducing host cells into a sealed container with multiple aseptic passages, performing genetic modification, and maintaining cells in controlled conditions monitored by real-time feedback until a desired fraction of genetically modified cells is achieved. The container also supports target cell isolation using buoyant microbubbles functionalized with antibodies or aptamers, facilitating separation of target cells from non-target cells during centrifugation. Pneumatic pressure is employed for waste separation and cell harvest, achieving high-purity cell recovery. The platform includes modules for cell enrichment, sequestration, and isolation, achieving high recovery and purity rates for therapeutic cell populations. This integrated, closed-system approach ensures aseptic conditions, efficient processing, and high-quality cell products for advanced therapeutic applications.

The invention relates to a method and platform for producing CAR-T cells and processing biological cells within a single, functionally closed container. The method involves introducing host cells into a sealed container with multiple aseptic passages, performing genetic modification, and maintaining cells in controlled conditions monitored by real-time feedback until a desired fraction of genetically modified cells is achieved. The container also supports target cell isolation using buoyant microbubbles functionalized with antibodies or aptamers, facilitating separation of target cells from non-target cells during centrifugation. Pneumatic pressure is employed for waste separation and cell harvest, achieving high-purity cell recovery. The platform includes modules for cell enrichment, sequestration, and isolation, achieving high recovery and purity rates for therapeutic cell populations. This integrated, closed-system approach ensures aseptic conditions, efficient processing, and high-quality cell products for advanced therapeutic applications.

The utility model relates to the field of cell culture, and discloses a cell harvesting support for cell autophagy, which comprises a bottom plate, the upper part of the top surface of the bottom plate is fixedly connected with a support body, the upper part of the support body is provided with a placing plate, the right side of the support body is fixedly connected with a motor, and the rear side of the placing plate is provided with a hook piece. A fixing piece is arranged on the front side of the containing plate, an auxiliary piece is arranged on the upper portion of the containing plate, vertical plates are fixedly connected to the left side and the right side of the top face of the containing plate, connecting grooves are formed in the left portion and the right portion of the front side of the top face of the containing plate, a sliding groove is formed in the middle of the rear side of the top face of the containing plate, and sliding openings are formed in the upper portions of the vertical plates. And the output end of the motor is fixedly connected with a rotating shaft. According to the cell culture device disclosed by the utility model, the placement plate is matched with the auxiliary part, and the telescopic rod is matched with the compression roller, so that cells in the cell culture bag can be better guided out, and the cell culture device is more practical.

The utility model discloses a suction tube convenient for cell harvesting and passage, which relates to the technical field of cell passage scraping, and adopts the technical scheme that the suction tube comprises a suction head, a conical tube and a thick-diameter tube, the top and the bottom of the thick-diameter tube are respectively connected with the suction head and the conical tube, and the suction tube is characterized in that a rotating device is arranged at the bottom of the conical tube; a suction pipe is arranged at the end, away from the conical pipe, of the rotating device; the rotating device comprises a spherical shell and a rotating spherical shell; the top of the spherical shell is connected with the conical pipe, and the large-diameter pipe, the conical pipe and the spherical shell are integrally formed. The outer side wall of the rotating spherical shell is rotationally connected with the inner side wall of the spherical shell, the top and the bottom of the rotating spherical shell are both provided with openings, and the position, away from the opening of the conical pipe, of the rotating spherical shell is connected with the suction pipe. According to the L-shaped plastic suction tube, the swinging angle of the suction tube can be controlled, cell colonies can be dispersed by using the outer side wall of the suction tube, and an operator can conveniently press the suction head to suck and transfer cells.

The invention relates to the technical field of biological materials, in particular to a plant source microcarrier, a preparation method and application. The invention discloses a soybean protein isolate-based cell culture microcarrier which is low in cost, high in safety and easy in cell recovery and a preparation method thereof, and is suitable for large-scale culture of adherent cells. The microcarrier takes soybean protein isolate as a matrix, the particle size of the solid microcarrier is 50-350 microns, and the density is 1.03 g / cm < 3 >. The solid soybean protein isolate microcarrier matrix material provided by the invention is plant-derived, avoids the risk of animal-derived pollution, is good in safety, can mildly and conveniently harvest cells in a manner of splitting the microcarrier by using the disperase II, and can still maintain high activity of the harvested cells, so that the downstream treatment operation after the cells are amplified by the microcarrier is simplified. The cell culture medium can support the growth of various types of cells, including VERO for vaccine production, SHED for regenerative medicine and C2C12 for artificial meat research, and has the potential of being used as a multi-purpose microcarrier in different cell culture fields.

The invention relates to a cell treatment method which comprises the following steps: S1, filling a centrifugal cup with a cleaning solution, namely rotating the centrifugal cup to keep a centrifugal state, introducing the cleaning solution into the centrifugal cup, and stopping pumping the cleaning solution after the centrifugal cup is filled with the cleaning solution; s2, continuous flow concentration: pumping the cell sample liquid into a centrifugal cup, enabling cells to be attached to the wall of the centrifugal cup under the action of centrifugal force, and discharging supernate out of the centrifugal cup; s3, cleaning the cell sample: pumping the cleaning solution into the centrifugal cup, after the centrifugal cup is filled with the cleaning solution, continuously pumping the cleaning solution with a set volume and synchronously pumping out a centrifugal supernatant with the same volume, and maintaining for a certain time; s4, scattering the cell sample: stopping the centrifugal state of the centrifugal cup, periodically rotating the centrifugal cup forwards and backwards, and scattering clustered cells in the centrifugal cup into single suspension cells; s5, cell resuspension: introducing the cell cryopreservation liquid into a centrifugal cup, and uniformly mixing the centrifugal cup in a positive and negative rotation manner to uniformly mix the cell suspension; s6, collecting the cells.

Methods and compositions related to Norovirus virus-like particles are provided. An example method comprises: providing a cell harvest containing host cells and Norovirus antigenic proteins, subjecting the cell harvest to a cell lysis step to obtain a cell lysate of the host cells, centrifuging the cell lysate to obtain a lysate supernatant, adding one or more precipitating agents to the lysate supernatant to obtain a supernatant fraction and a precipitate fraction of the lysate supernatant, subjecting the precipitation fraction to a separation step to obtain a retentate product, redissolving the Norovirus VLPs of the retentate product in a buffer to obtain a buffer solution containing the Norovirus VLPs, centrifuging the buffer solution to obtain a supernatant thereof, subjecting the supernatant of the buffer solution to one or more chromatography steps to obtain a chromatography product, and subjecting the chromatography product to a combined ultrafiltration and diafiltration (UF / DF) step.