20 results about "Cell harvest" patented technology

The present invention relates to a method and platform for generating CAR-T cells and processing biological cells within a single functionally closed container. The method involves introducing host cells into a sealed container with multiple sterile channels, performing genetic modifications, and maintaining the cells under controlled conditions monitored by real-time feedback until a desired fraction of genetically modified cells is obtained. The container also supports target cell separation using antibody or aptamer functionalized buoyant microbubbles, facilitating the separation of target cells from non-target cells during centrifugation. Waste separation and cell harvesting are performed using pneumatic pressure, enabling high purity cell recovery. The platform includes modules for cell enrichment, sequestration, and isolation, enabling high recovery and high purity of therapeutic cell populations. This integrated closed system approach ensures sterile conditions, efficient processing, and high quality cell products for advanced therapeutic applications.

The invention discloses a full-automatic cell harvesting system and a harvesting control method.The harvesting system comprises a centrifugal bin used for executing centrifugal action, and the centrifugal bin is provided with a liquid inlet channel communicated to the position close to the top of the side wall of a bin body and a liquid outlet channel connected to the bottom wall of the bin body; the liquid adding unit is connected with the liquid inlet channel and comprises a sample branch, a cleaning branch, a resuspension branch and a liquid adding pump; the recovery unit is connected with the liquid outlet channel and comprises a finished product branch, a waste liquid branch and a recovery pump; and the visual detection module is triggered by a pulse signal to carry out image acquisition at a preset frequency, and the pulse signal adjusts the triggering frequency according to the rotating speed of the centrifugal bin. Through cooperation of the centrifugal bin, the liquid adding unit and the recycling unit, full-automatic harvesting of cells is achieved, and the high-throughput cell harvesting requirement can be met.

A method for producing and applying cellular materials from human cells for non-therapeutic applications is provided. The method comprises the steps of obtaining human cells, cultivating the human cells in a controlled environment, harvesting the cultivated human cells, processing the cultivated human cells to produce cellular materials and applying the cellular materials to a human subject for non-therapeutic applications. Other example embodiments are described herein. In certain embodiments, the method offers an environmentally sustainable solution that provides human cellular materials having improved safety, bioactivity, and batch consistency for human use.

1. Name of the design product: bioreactor. 2. Use of the design product: the product is an integrated bioreactor, which can complete cell culture (primary culture), expansion culture, cell culture from tissues, medium replacement and cell harvesting. 3. Design points of the design product: in shape. 4. Picture or photo best indicating the design points: front view.

This utility model discloses a cell harvester with an automatic sample introduction mechanism, including a chamber and test tubes. A centrifugation mechanism assembly is installed at the top of the chamber, and an automatic sample introduction component is installed at the top of the chamber, directly above the centrifugation mechanism assembly. A sample tube rack is installed at the top of the chamber, and placement slots are evenly distributed at the top of the sample tube rack. Test tubes are placed inside the placement slots. A rotating disk is rotatably connected inside the placement slots, and an arc-shaped groove is evenly distributed around the top of the rotating disk. Multiple sets of clamping rods are installed at the top of the rotating disk, and a connecting shaft adapted to the arc-shaped groove is fixedly installed at the bottom of the clamping rods. A cavity is opened inside the sample tube rack. In this utility model, by pulling the pulling rod, the limiting block is disengaged from the limiting slot, and the adjusting block can be slid. The rotating disk is driven by rack and pinion transmission, so that multiple sets of clamping rods move synchronously, flexibly adapting to test tubes of different sizes and increasing the convenience of use.

The invention particularly relates to the technical field of stem cell preparation and tissue engineering, and discloses a placenta mesenchymal stem cell multi-source extraction system based on an integrated treatment platform. Comprising a multi-region synchronous pretreatment module, a zoning adherent culture module, a differentiated cell harvesting module, a cell characteristic collection module, a functional cell division module and a whole-process closed-loop control module, the multi-region synchronous pretreatment module adopts an integrated design, and the zoning adherent culture module realizes centralized culture through an independent marking region; the differentiation cell harvesting module is used for carrying out merging amplification; the cell characteristic acquisition module is used for acquiring cell characteristic data; the functional cell division module is used for realizing cell type and function judgment; the whole-process closed-loop control module is used for realizing whole-process quality tracing; through the integrated design, the cost is reduced, the cell quality stability is ensured, and the whole-process quality tracing is realized.

The utility model discloses automatic production equipment for cell drugs. The automatic production equipment comprises a process operation cavity and a cell factory incubator, the cell factory incubator is communicated with the process operation cavity; the process operation cavity is provided with a cell harvesting area and a cell inoculation liquid changing area; a centrifugal bottle pipetting and cover opening and closing assembly, a centrifugal assembly, a subpackaging assembly and a first transfer mechanism are arranged in the cell harvesting area; the first transfer mechanism is used for transferring the centrifugal bottle between the centrifugal bottle pipetting and cover opening and closing assembly and the centrifugal assembly; a cell factory pipetting and cover opening and closing assembly and a second transfer mechanism are arranged in the cell inoculation liquid changing area; the second transfer mechanism is used for transferring the cell factory among the cell factory pipetting and cover opening and closing assembly, the cell factory incubator and the centrifugal bottle pipetting and cover opening and closing assembly. By means of the arrangement, the function of full-process automation is achieved, and the purposes of reducing manual intervention and improving operation efficiency and sterility are achieved.

The utility model relates to cell medical field discloses a kind of for cell liquid supplement and cell suspension collection device, including collection bottle, filter screen, sealing ring and injection assembly, the filter screen is slidably connected in the collection bottle top, the handle is fixedly connected in the filter screen inside, the sealing ring is slidably connected in the collection bottle outside, the collection bottle top and the sealing ring inside are all fixedly connected with four magnets, the injection assembly is installed in the sealing ring top, the positioning assembly is installed in the injection assembly outside, the injection assembly includes pipeline, and the pipeline output end is fixedly connected in the sealing ring top.In the utility model, by pulling slide bar, piston compression spring one is driven, simultaneously under the cooperation of two check valves, after supplement liquid, cell suspension is inhaled into shell from pipeline left end, then slide bar is pushed, and it will be transported to collection bottle along pipeline right side, and liquid supplement or cell harvesting can be realized quickly, and it is transported to collection bottle, and the equipment structure is simple, easy to operate, and low in operating cost.

A three-dimensional porous growth surface (carriers) and cell culture devices including the same are provided. The carriers are made by multiple layers of netting or mesh, especially large dimension and area of fabrics that are capable to form a column-type fixed bed by rolling the layers or other shape of fixed bed by stacking or randomly disposed packing the carriers to form a packed-bed for cell culture the layers together, thereby sealing the surrounding of the multilayer nettings or meshes to reduce particle generation during cell harvest, or ease of separation by filtration due to larger wall dimension on the nettings or meshes than cells. The fixed bed make by large dimension of the growth surface can easily to manufacture a fixed bed simply by rolling the multiple layers of sheets, which can reduce the manufacture cost and also facilitate mass production of carriers for fixed bed bioreactors.

1. Name of the design product: bioreactor. 2. Use of the design product: the product is an integrated bioreactor, which can complete cell culture (primary culture), expansion culture, cell culture from tissues, medium replacement and cell harvesting. 3. Design points of the design product: in shape. 4. Picture or photo best indicating the design points: front view.

The present application relates to the technical field of cell culture and cell harvesting, and in particular to a lysis powder and its application in lysis of gelatin-based microcarriers. The present application comprises a lysis enzyme module, an EDTA chelation module and a cell protection module, which can realize rapid lysis of gelatin-based microcarriers and release of cells, improve the proportion of live cells in the harvested cells, and maintain the ability of the harvested cells to adhere, recover and proliferate after being reseeded, thereby being suitable for cell engineering, tissue engineering, cell culture meat and related large-scale production scenarios.

Provided is a cell harvesting device. The cell harvesting apparatus comprises: an agitator adapted to receive a bioreactor; and a connector adapted to couple the agitator to the bioreactor.

Disclosed herein is a cell harvesting instrument suitable for concentrating and / or washing cells from a source suspension of cells, the instrument comprising: a housing for accommodating mechanical elements comprising at least one fluid pump, at least one valve; and a treatment kit removably inserted into the housing, the kit comprising a substantially flat frame having or supporting a plurality of sealed fluid paths, the fluid paths being arranged in a substantially plane and such that fluid in the paths does not contact the mechanical element, where at least a portion of the fluid paths comprises a flexible tube, the flexible tube being configured to receive fluid from the fluid paths. The outer surface of the flexible tube may be manipulated by the or each fluid pump to provide fluid flow in one or more of the paths, and / or by the or each valve to constrain fluid flow in one or more of the paths. In an embodiment, the kit also includes a fluid handling reservoir and a filter adapted to separate cells from the fluid in the pathway. Transfer mechanisms for moving and weighing fluid processing reservoirs are also disclosed.

The present invention provides improved devices for biopsy, aspiration, stem cell acquisition, and methods of using the same. The devices balance aspiration with concurrent infusion to manage changes in pressure at the site of biopsy. The present invention can be adapted for any biopsy, aspiration, or cell harvest procedure, including adipose tissue aspiration and bone marrow aspiration (BMA). In particular, the present invention limits patient pain, prevents blood contamination, and increases cell mobilization, such as improved stem cell yields with intraosseous (10) pharmacological mobilization of stem cells during a BMA procedure, and with improved stem cell yields using pharmacological mobilization of stem cells from fat. The pharmacological mobilization of cells allows the harvest of cells from a biopsy many fold larger than existing methods.

The utility model discloses a suction tube convenient for cell harvesting and passage, which relates to the technical field of cell passage scraping, and adopts the technical scheme that the suction tube comprises a suction head, a conical tube and a thick-diameter tube, the top and the bottom of the thick-diameter tube are respectively connected with the suction head and the conical tube, and the suction tube is characterized in that a rotating device is arranged at the bottom of the conical tube; a suction pipe is arranged at the end, away from the conical pipe, of the rotating device; the rotating device comprises a spherical shell and a rotating spherical shell; the top of the spherical shell is connected with the conical pipe, and the large-diameter pipe, the conical pipe and the spherical shell are integrally formed. The outer side wall of the rotating spherical shell is rotationally connected with the inner side wall of the spherical shell, the top and the bottom of the rotating spherical shell are both provided with openings, and the position, away from the opening of the conical pipe, of the rotating spherical shell is connected with the suction pipe. According to the L-shaped plastic suction tube, the swinging angle of the suction tube can be controlled, cell colonies can be dispersed by using the outer side wall of the suction tube, and an operator can conveniently press the suction head to suck and transfer cells.

The invention relates to the technical field of biological materials, in particular to a plant source microcarrier, a preparation method and application. The invention discloses a soybean protein isolate-based cell culture microcarrier which is low in cost, high in safety and easy in cell recovery and a preparation method thereof, and is suitable for large-scale culture of adherent cells. The microcarrier takes soybean protein isolate as a matrix, the particle size of the solid microcarrier is 50-350 microns, and the density is 1.03 g / cm < 3 >. The solid soybean protein isolate microcarrier matrix material provided by the invention is plant-derived, avoids the risk of animal-derived pollution, is good in safety, can mildly and conveniently harvest cells in a manner of splitting the microcarrier by using the disperase II, and can still maintain high activity of the harvested cells, so that the downstream treatment operation after the cells are amplified by the microcarrier is simplified. The cell culture medium can support the growth of various types of cells, including VERO for vaccine production, SHED for regenerative medicine and C2C12 for artificial meat research, and has the potential of being used as a multi-purpose microcarrier in different cell culture fields.

The utility model relates to a blending device and cell harvesting equipment, which comprises a support plate, a hook is arranged on the support plate, a push plate parallel to the support plate is arranged at the lower part of the support plate, the hook is used for hanging a cell sample bag, and the push plate can move relative to the support plate so as to extrude the cell sample bag. The cell harvesting equipment comprises a machine base, a hanging frame and the uniform mixing device, a centrifugal cup containing cavity is formed in the machine base, a plurality of liquid storage bags are hung on the hanging frame, and the centrifugal cup containing cavity is used for containing the centrifugal cups.

Disclosed herein is a cell harvesting instrument suitable for concentrating and / or washing cells from a source suspension of cells, the instrument comprising: a housing for accommodating mechanical elements comprising at least one fluid pump, at least one valve; and a treatment kit removably inserted into the housing, the kit comprising a substantially flat frame having or supporting a plurality of sealed fluid paths, the fluid paths being arranged in a substantially plane and such that fluid in the paths does not contact the mechanical element, where at least a portion of the fluid paths comprises a flexible tube, the flexible tube being configured to receive fluid from the fluid paths. The outer surface of the flexible tube may be manipulated by the or each fluid pump to provide fluid flow in one or more of the paths, and / or by the or each valve to constrain fluid flow in one or more of the paths. In an embodiment, the kit also includes a fluid handling reservoir and a filter adapted to separate cells from the fluid in the pathway. Transfer mechanisms for moving and weighing fluid processing reservoirs are also disclosed.

The present application relates to cell harvester equipment technical field, especially to a kind of centrifugal tube taking and fixing mechanism of full-automatic cell harvester.It includes disc-shaped tube rack disc, the center of tube rack disc is provided with lock rod, multiple centrifugal tube mounting holes are uniformly distributed around the lock rod, centrifugal tube mounting hole is used to install centrifugal tube, the lock rod is passed through tube rack disc and can be axially moved, the upper end of lock rod is hinged with pressure rod, and pressure rod is hinged on tube rack disc by connecting rod;The lower end of the lock rod is provided with push mechanism for pushing the axial movement of the lock rod.The present application can cooperate with mechanical arm to realize automatic placement of centrifugal tube, and adopts automatic mechanical device to press or release centrifugal tube.The automation degree of centrifugal tube taking and pressing is high, and manual operation is not needed, to provide technical support for realizing full-automatic cell harvester without manual operation.

The invention discloses a method for controlling cell sorting culture. The method comprises the following steps: S1, sorting preparation: adding magnetic beads and initial cell sap into a container; s2, cell sorting: applying a magnetic field to the container to enrich the magnetic beads on the container, and discharging waste liquid; s3, cell culture: removing the magnetic field on the container, and adding a culture solution into the container for culture; s4, harvesting cells: harvesting target cells after cell culture is completed. The invention also discloses a cell sorting culture system and a consumable for cell sorting culture. The method, the system and the consumables for controlling cell sorting culture have the advantage of convenience of cell sorting and culture integration automation.