13 results about "Cell harvest" patented technology

The present invention relates to a method and platform for generating CAR-T cells and processing biological cells within a single functionally closed container. The method involves introducing host cells into a sealed container with multiple sterile channels, performing genetic modifications, and maintaining the cells under controlled conditions monitored by real-time feedback until a desired fraction of genetically modified cells is obtained. The container also supports target cell separation using antibody or aptamer functionalized buoyant microbubbles, facilitating the separation of target cells from non-target cells during centrifugation. Waste separation and cell harvesting are performed using pneumatic pressure, enabling high purity cell recovery. The platform includes modules for cell enrichment, sequestration, and isolation, enabling high recovery and high purity of therapeutic cell populations. This integrated closed system approach ensures sterile conditions, efficient processing, and high quality cell products for advanced therapeutic applications.

The invention discloses a full-automatic cell harvesting system and a harvesting control method.The harvesting system comprises a centrifugal bin used for executing centrifugal action, and the centrifugal bin is provided with a liquid inlet channel communicated to the position close to the top of the side wall of a bin body and a liquid outlet channel connected to the bottom wall of the bin body; the liquid adding unit is connected with the liquid inlet channel and comprises a sample branch, a cleaning branch, a resuspension branch and a liquid adding pump; the recovery unit is connected with the liquid outlet channel and comprises a finished product branch, a waste liquid branch and a recovery pump; and the visual detection module is triggered by a pulse signal to carry out image acquisition at a preset frequency, and the pulse signal adjusts the triggering frequency according to the rotating speed of the centrifugal bin. Through cooperation of the centrifugal bin, the liquid adding unit and the recycling unit, full-automatic harvesting of cells is achieved, and the high-throughput cell harvesting requirement can be met.

A method for producing and applying cellular materials from human cells for non-therapeutic applications is provided. The method comprises the steps of obtaining human cells, cultivating the human cells in a controlled environment, harvesting the cultivated human cells, processing the cultivated human cells to produce cellular materials and applying the cellular materials to a human subject for non-therapeutic applications. Other example embodiments are described herein. In certain embodiments, the method offers an environmentally sustainable solution that provides human cellular materials having improved safety, bioactivity, and batch consistency for human use.

1. Name of the design product: bioreactor. 2. Use of the design product: the product is an integrated bioreactor, which can complete cell culture (primary culture), expansion culture, cell culture from tissues, medium replacement and cell harvesting. 3. Design points of the design product: in shape. 4. Picture or photo best indicating the design points: front view.

This utility model discloses a cell harvester with an automatic sample introduction mechanism, including a chamber and test tubes. A centrifugation mechanism assembly is installed at the top of the chamber, and an automatic sample introduction component is installed at the top of the chamber, directly above the centrifugation mechanism assembly. A sample tube rack is installed at the top of the chamber, and placement slots are evenly distributed at the top of the sample tube rack. Test tubes are placed inside the placement slots. A rotating disk is rotatably connected inside the placement slots, and an arc-shaped groove is evenly distributed around the top of the rotating disk. Multiple sets of clamping rods are installed at the top of the rotating disk, and a connecting shaft adapted to the arc-shaped groove is fixedly installed at the bottom of the clamping rods. A cavity is opened inside the sample tube rack. In this utility model, by pulling the pulling rod, the limiting block is disengaged from the limiting slot, and the adjusting block can be slid. The rotating disk is driven by rack and pinion transmission, so that multiple sets of clamping rods move synchronously, flexibly adapting to test tubes of different sizes and increasing the convenience of use.

The utility model discloses automatic production equipment for cell drugs. The automatic production equipment comprises a process operation cavity and a cell factory incubator, the cell factory incubator is communicated with the process operation cavity; the process operation cavity is provided with a cell harvesting area and a cell inoculation liquid changing area; a centrifugal bottle pipetting and cover opening and closing assembly, a centrifugal assembly, a subpackaging assembly and a first transfer mechanism are arranged in the cell harvesting area; the first transfer mechanism is used for transferring the centrifugal bottle between the centrifugal bottle pipetting and cover opening and closing assembly and the centrifugal assembly; a cell factory pipetting and cover opening and closing assembly and a second transfer mechanism are arranged in the cell inoculation liquid changing area; the second transfer mechanism is used for transferring the cell factory among the cell factory pipetting and cover opening and closing assembly, the cell factory incubator and the centrifugal bottle pipetting and cover opening and closing assembly. By means of the arrangement, the function of full-process automation is achieved, and the purposes of reducing manual intervention and improving operation efficiency and sterility are achieved.

The utility model relates to cell medical field discloses a kind of for cell liquid supplement and cell suspension collection device, including collection bottle, filter screen, sealing ring and injection assembly, the filter screen is slidably connected in the collection bottle top, the handle is fixedly connected in the filter screen inside, the sealing ring is slidably connected in the collection bottle outside, the collection bottle top and the sealing ring inside are all fixedly connected with four magnets, the injection assembly is installed in the sealing ring top, the positioning assembly is installed in the injection assembly outside, the injection assembly includes pipeline, and the pipeline output end is fixedly connected in the sealing ring top.In the utility model, by pulling slide bar, piston compression spring one is driven, simultaneously under the cooperation of two check valves, after supplement liquid, cell suspension is inhaled into shell from pipeline left end, then slide bar is pushed, and it will be transported to collection bottle along pipeline right side, and liquid supplement or cell harvesting can be realized quickly, and it is transported to collection bottle, and the equipment structure is simple, easy to operate, and low in operating cost.

A three-dimensional porous growth surface (carriers) and cell culture devices including the same are provided. The carriers are made by multiple layers of netting or mesh, especially large dimension and area of fabrics that are capable to form a column-type fixed bed by rolling the layers or other shape of fixed bed by stacking or randomly disposed packing the carriers to form a packed-bed for cell culture the layers together, thereby sealing the surrounding of the multilayer nettings or meshes to reduce particle generation during cell harvest, or ease of separation by filtration due to larger wall dimension on the nettings or meshes than cells. The fixed bed make by large dimension of the growth surface can easily to manufacture a fixed bed simply by rolling the multiple layers of sheets, which can reduce the manufacture cost and also facilitate mass production of carriers for fixed bed bioreactors.

1. Name of the design product: bioreactor. 2. Use of the design product: the product is an integrated bioreactor, which can complete cell culture (primary culture), expansion culture, cell culture from tissues, medium replacement and cell harvesting. 3. Design points of the design product: in shape. 4. Picture or photo best indicating the design points: front view.

The present application relates to the technical field of cell culture and cell harvesting, and in particular to a lysis powder and its application in lysis of gelatin-based microcarriers. The present application comprises a lysis enzyme module, an EDTA chelation module and a cell protection module, which can realize rapid lysis of gelatin-based microcarriers and release of cells, improve the proportion of live cells in the harvested cells, and maintain the ability of the harvested cells to adhere, recover and proliferate after being reseeded, thereby being suitable for cell engineering, tissue engineering, cell culture meat and related large-scale production scenarios.

Disclosed herein is a cell harvesting instrument suitable for concentrating and / or washing cells from a source suspension of cells, the instrument comprising: a housing for accommodating mechanical elements comprising at least one fluid pump, at least one valve; and a treatment kit removably inserted into the housing, the kit comprising a substantially flat frame having or supporting a plurality of sealed fluid paths, the fluid paths being arranged in a substantially plane and such that fluid in the paths does not contact the mechanical element, where at least a portion of the fluid paths comprises a flexible tube, the flexible tube being configured to receive fluid from the fluid paths. The outer surface of the flexible tube may be manipulated by the or each fluid pump to provide fluid flow in one or more of the paths, and / or by the or each valve to constrain fluid flow in one or more of the paths. In an embodiment, the kit also includes a fluid handling reservoir and a filter adapted to separate cells from the fluid in the pathway. Transfer mechanisms for moving and weighing fluid processing reservoirs are also disclosed.

Disclosed herein is a cell harvesting instrument suitable for concentrating and / or washing cells from a source suspension of cells, the instrument comprising: a housing for accommodating mechanical elements comprising at least one fluid pump, at least one valve; and a treatment kit removably inserted into the housing, the kit comprising a substantially flat frame having or supporting a plurality of sealed fluid paths, the fluid paths being arranged in a substantially plane and such that fluid in the paths does not contact the mechanical element, where at least a portion of the fluid paths comprises a flexible tube, the flexible tube being configured to receive fluid from the fluid paths. The outer surface of the flexible tube may be manipulated by the or each fluid pump to provide fluid flow in one or more of the paths, and / or by the or each valve to constrain fluid flow in one or more of the paths. In an embodiment, the kit also includes a fluid handling reservoir and a filter adapted to separate cells from the fluid in the pathway. Transfer mechanisms for moving and weighing fluid processing reservoirs are also disclosed.

The present application relates to cell harvester equipment technical field, especially to a kind of centrifugal tube taking and fixing mechanism of full-automatic cell harvester.It includes disc-shaped tube rack disc, the center of tube rack disc is provided with lock rod, multiple centrifugal tube mounting holes are uniformly distributed around the lock rod, centrifugal tube mounting hole is used to install centrifugal tube, the lock rod is passed through tube rack disc and can be axially moved, the upper end of lock rod is hinged with pressure rod, and pressure rod is hinged on tube rack disc by connecting rod;The lower end of the lock rod is provided with push mechanism for pushing the axial movement of the lock rod.The present application can cooperate with mechanical arm to realize automatic placement of centrifugal tube, and adopts automatic mechanical device to press or release centrifugal tube.The automation degree of centrifugal tube taking and pressing is high, and manual operation is not needed, to provide technical support for realizing full-automatic cell harvester without manual operation.