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Method for sorting microalgae at high throughput

A high-throughput, microalgae technology, applied in unicellular algae and other directions, to achieve the effects of less cell damage, low cost, and mild operating conditions

Active Publication Date: 2015-07-15
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, most of the microalgae are harvested by foam separation technology or air flotation separation method to harvest all the biomass at one time, and there are no reports on the patent of flotation of microalgae with high content of the target product using the difference in surface properties.

Method used

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  • Method for sorting microalgae at high throughput

Examples

Experimental program
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Effect test

Embodiment 1

[0027] 1. Algae species: select Chlorella, isolated from the freshwater waters of southern Xinjiang;

[0028] 2. Synchronized culture: 250mL Erlenmeyer flask was inoculated with 150mL culture solution in shake flask culture, the initial inoculation concentration was 1×10 6 Cells / mL, 14h / 10h light and dark cycle culture Chlorella to achieve synchronized culture. The reactor used is an open or closed reactor; the light source used is sunlight, incandescent lamp, LED lamp, etc.; the cultivation method is autotrophic cultivation.

[0029] 3. Selective hydrophobization in batches: the microalgae cells in the logarithmic phase, stable phase and recession phase were taken to measure the oil content after cultured to the 5th day, 10th day and 15th day, and the oil content of each stage was 33%, 38%, and 43% respectively . Take microalgae cells to adjust the concentration of algae liquid to 1g / L, and add 0.005, 0.01, 0.03, 0.05g / LAl to 100mL of cells at each stage 2 (SO 4 ) 3 18H ...

Embodiment 2

[0031] Embodiment 2 (all the other steps are with embodiment 1)

[0032] 1. Microalgae culture: use 0.6m under natural light 2 Culture Chlorella in plastic pots for 10 days to obtain heterogeneous cells with an average oil content of 35%;

[0033] 2. Pre-hydrophobic treatment: add 0.01g / L Al 2 (SO 4 ) 3 18H 2 O, stir rapidly for 2min;

[0034] 3. Flotation: Harvest the microalgae biomass that floats out within 0 to 2 minutes by dissolved air flotation. The biomass accounts for 1 / 3 of the total biomass, and the oil content of the biomass reaches 39%.

Embodiment 3

[0035] Embodiment 3 (all the other steps are with embodiment 1)

[0036] 1. Microalgae culture: use 0.6m under natural light 2 Chlorella was cultured in plastic pots for 7 days to obtain heterogeneous cells with an average oil content of 26%.

[0037] 2. Pre-hydrophobic treatment: add 0.01g / L Al 2 (SO 4 ) 3 18H 2 O, stir rapidly for 2min.

[0038] 3. Flotation: Harvest the microalgae biomass that floats out within 0 to 2 minutes by dissolved air flotation. The biomass accounts for 1 / 3 of the total biomass, and the oil content of the biomass reaches 36%.

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Abstract

The invention belongs to the biotechnical field of microalgae and relates to a method for sorting microalgae at high throughput. The method specifically comprises the following steps: (1) culturing microalgae: (2) adding chemicals to selectively hydrophobize algae cells; (3) collecting the algae in an air floating method; and (4) detecting the content of target contents of microalgae cells harvested each period of time. The method provided by the invention has the advantages and positive effects that (1) the method is simple to operate and low in cost; 2, the method is suitable for sorting and harvesting various economical microalgae; 3, the microalgae cells with high content of targeted products can be quickly obtained in batches; and 4, the method is mild in operating condition and hardly damages cells.

Description

technical field [0001] The invention belongs to the technical field of microalgae, and relates to a method for sorting microalgae with high throughput. Background technique [0002] Microalgae are single-celled organisms with high photosynthetic efficiency, easy cultivation, short growth cycle, and high biomass production. Moreover, microalgae are rich in protein, fat, carbohydrates and other biologically active substances. They are an important class of biological resources and have excellent application prospects in industrial fields such as health food, biological bait, medicine and biofuel. For example, Chlorella, Crescent algae, Spirulina, etc. have been used as protein sources; Chlorella, Dunaliella salina, Haematococcus pluvialis, etc. rich in polyunsaturated fatty acids have been used as health food and pharmaceutical industries; Chlorella, Nannochloropsis. However, the key problem in the development of microalgae industrialization is the high production cost, and ...

Claims

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Application Information

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IPC IPC(8): C12N1/12
CPCC12N1/12
Inventor 宋少先夏令李洪强赵云良刘艳艳
Owner WUHAN UNIV OF TECH
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