Separation culture method of dental pulp stem cells

A technique for the separation and culture of dental pulp stem cells, applied to non-embryonic pluripotent stem cells, animal cells, vertebrate cells, etc., can solve the problems of unsatisfactory and lack of dental pulp stem cell culture effect, and improve the separation and culture efficiency of dental pulp stem cells. , the effect of reducing production costs

Inactive Publication Date: 2017-06-06
GUIZHOU BEIKE FACTORR BIOTECH CO LTD
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, at present, there is a lack of a complete system for the cultivation of dental pulp stem cells, and the cultivation effect of dental pulp stem cells is not ideal.

Method used

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  • Separation culture method of dental pulp stem cells
  • Separation culture method of dental pulp stem cells
  • Separation culture method of dental pulp stem cells

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Embodiment Construction

[0020] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0021] The invention provides a method for isolating and culturing dental pulp stem cells. By establishing a complete system for isolating and culturing dental pulp stem cells, the culture efficiency of dental pulp stem cells is improved, which includes the following steps.

[0022] like figure 1 , S1, Isolate stem cells: Wrap the tooth with sterilized gauze, place it in a vise, crush the tooth to expose the pulp tissue, cut the pulp tissue with ...

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Abstract

The invention relates to the field of separation culture of dental pulp stem cells, and discloses a separation culture method of the dental pulp stem cells. The method comprises the following steps: crushing a tooth to expose dental pulp tissues, cutting the dental pulp tissues into pieces, collecting into a centrifuge tube, and using collagenase type I and neutral protease to decompose the dental pulp tissues; adding a complete culture solution to finish the decomposition, centrifuging to remove supernatant, then resuspending cells by using the complete culture solution, and filtering to obtain primary cell suspension liquid; adopting 1*104/cm<2> as primary cell planting density; two days later, changing the liquid for the first time, then changing the liquid for once every three days, culturing for 9-19 days, wherein about 100 clone cells are formed by each primary cell, and carrying out cell harvesting and passage; planting passage cells according to the planting density of 1*4000/cm<2>, and carrying out cell harvesting and passage again when the cells reach 70-90% of convergence degree. The separation culture method increases the separation culture efficiency and yield of the dental pulp stem cells and reduces the production cost by regulating the separation culture method of the dental pulp stem cells and all parameters in the concrete operation.

Description

technical field [0001] The invention relates to the field of isolating and culturing dental pulp stem cells, in particular to a method for isolating and culturing dental pulp stem cells. Background technique [0002] The stem cell storage business has developed from simple umbilical cord blood storage to the current storage of stem cells from various sources such as umbilical cord, placenta, and teeth, and its importance and necessity have been recognized by more and more people. However, there are still quite a few children who lost the first opportunity to store umbilical cord blood stem cells or umbilical cord stem cells for their children due to their parents’ lack of knowledge about stem cells in the past. The development of deciduous stem cell storage business can make up for this regret. Buy an insurance policy for your child's health. Dental pulp stem cells can be used in pulp regeneration, bone regeneration, nerve cell regeneration, cardiac muscle, blood vessel reg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/074C12N5/0775
CPCC12N5/0664
Inventor 张凤宁
Owner GUIZHOU BEIKE FACTORR BIOTECH CO LTD
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