Methods, devices, arrays and kits for detecting and analyzing biomolecules

a biomolecule and array technology, applied in the field of biomolecule detection and analysis methods, can solve the problems of low biomolecule specificity and high affinity of certain membranes used in such methods, and achieve the effect of increasing specificity
US20040081979A1Inactive Publication Date: 2004-04-29HEALTH & HUMAN SERVICES THE US SEC +1
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
HEALTH & HUMAN SERVICES THE US SEC
Publication Date
2004-04-29
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present disclosure is directed to devices, arrays, kits and methods for detecting biomolecules in a tissue section (such as a fresh or archival sample, tissue microarray, or cells harvested by an LCM procedure) or other substantially two-dimensional sample (such as an electrophoretic gel or cDNA microarray) by creating "carbon copies" of the biomolecules eluted from the sample and visualizing the biomolecules on the copies using one or more detector molecules (e.g., antibodies or DNA probes) having specific affinity for the biomolecules of interest. Specific methods are provided for identifying the pattern of biomolecules (e.g., proteins and nucleic acids) in the samples. Other specific methods are provided for the identification and analysis of proteins and other biological molecules produced by cells and / or tissue, especially human cells and / or tissue. The disclosure also provides a plurality of differentially prepared and / or processed membranes that can be used in described methods, and which permit the identification and analysis of biomolecules.
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Description

REFERENCE TO RELATED APPLICATIONS

[0001] This application is a Continuation in Part (CIP) of U.S. patent application Ser. No. 09 / 753,574 (filed Jan. 4, 2001), which is a CIP of U.S. patent application Ser. No. 09 / 718,990 (Filed Nov. 20, 2000), which is a CIP of International Patent Application No. US00 / 20354, filed Jul. 26, 2000 and published in the English language, and claims the benefit of U.S. Provisional Patent Application No. 60 / 145,613 (filed Jul. 26, 1999). The current application further claims the benefit of U.S. Provisional Patent Application Nos. 60 / 286,258 (filed Apr. 25, 2001), 60 / 304,031 (filed Jul. 9, 2001), and 60 / 296,475 (filed Jun. 8, 2001). Each of these related applications is incorporated herein in their entirety.FIELD OF THE DISCLOSURE

[0003] The present disclosure is directed to methods, devices, arrays, and kits for identifying and analyzing large numbers of biomolecules in a sample, such as a biological sample. The disclosure further relates to using these me...

Claims

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