Separation culture method for endothelial progenitor cells and kit of method

An endothelial progenitor cell, separation and culture technology, which is applied in the field of the separation and culture method of umbilical cord blood endothelial progenitor cells and a kit thereof, can solve the problems of limited endothelial progenitor cells, low amplification efficiency, slow cell proliferation and the like, and achieves cell surface antigen The effect of high expression rate, large cell harvest, and high cell confluency
CN104845935AInactive Publication Date: 2015-08-19GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
Publication Date
2015-08-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the technical field of separation culture of endothelial progenitor cells of umbilical blood, particularly a separation culture method for endothelial progenitor cells of umbilical blood and a kit of the method. The method comprises the following steps: carrying out adherent culture on a mononuclear cell of umbilical blood in advance; and carrying out cell culture on non-adherent cells, wherein a culture medium for cell culture is a full culture medium containing fetuin. Compared to cells obtained by conventional methods, the endothelial progenitor cells of umbilical blood cultured by the method provided by the invention are relatively uniform in shape, relatively high in convergence degree, relatively great in cell harvest yield, relatively fast in cell proliferation and relatively high in antigen presentation rate on cell surface.
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Description

technical field

[0001] The invention relates to the technical field of separation and culture of endothelial progenitor cells, in particular to a method for separation and culture of umbilical cord blood endothelial progenitor cells and a kit thereof. Background technique

[0002] Cardiovascular and cerebrovascular diseases are collectively referred to as cardiovascular and cerebrovascular diseases, and generally refer to ischemic or hemorrhagic diseases in the heart, brain, and systemic tissues caused by hyperlipidemia, blood viscosity, atherosclerosis, and high blood pressure. . It is a common disease that seriously threatens the health of human beings, especially middle-aged and elderly people over 50 years old. Even with the most advanced and perfect treatment methods, more than 50% of the survivors of cerebrovascular accidents still cannot take care of themselves completely. The number of people dying from cardiovascular and cerebrovascular diseases is as high as 15 mi...

Claims

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