Filling preparation and preparation method thereof

A preparation and fat particle technology, applied in the field of filling preparations and their preparation, can solve the problems of short maintenance time, low survival rate, poor filling effect, etc., and achieve the effects of small rejection reaction, improved survival rate, and high safety factor for use

Pending Publication Date: 2019-09-17
CHENGDU QINGKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a filling preparation and a preparation method thereof, which can effectively solve the problems of low survival rate, short maintenance time after transplantation and poor filling effect existing in existing filling materials

Method used

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  • Filling preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A kind of filling preparation, its preparation method comprises the following steps:

[0028] (1) Collect peripheral blood, centrifuge at 800g / min for 6min, discard the platelet-poor plasma in the upper section and red blood cells in the lower section, store the platelet-rich plasma in the middle section at -40°C for 3 hours, take out the freeze-thaw, and then freeze and thaw the platelet-rich plasma Plasma was centrifuged at 2000rpm / min for 6min to remove membrane fragments at the bottom to obtain platelet factor-rich plasma for later use;

[0029] (2) Absorb autologous subcutaneous fat, let it stand still, discard the upper layer of fat, and wash it twice with sterile saline to obtain fat particles. Take half of the fat particles, add DMSO freezing solution of 1 times the volume of fat particles to it, and remove the fat particles. Carry out gradient cooling and freeze storage, then resuscitate the fat granules and wash away the DMSO freezing solution in the fat granu...

Embodiment 2

[0033] A kind of filling preparation, its preparation method comprises the following steps:

[0034] (1) Collect peripheral blood, centrifuge at 2000g / min for 12min, discard the platelet-poor plasma in the upper section and red blood cells in the lower section, store the platelet-rich plasma in the middle section at -80°C for 8 hours, take out the freeze-thaw, and then freeze and thaw the platelet-rich plasma Plasma was centrifuged at 4000rpm / min for 10min to remove membrane fragments at the bottom to obtain platelet factor-rich plasma for later use;

[0035] (2) Absorb autologous subcutaneous fat, let it stand, discard the upper layer of fat and wash it with sterile saline for 3 times to obtain fat particles, take half of the fat particles, add 4 times the volume of fat particles to DMSO freezing liquid, and remove the fat particles Carry out gradient cooling and cryopreservation, then resuscitate the fat particles and wash away the DMSO freezing solution in the fat particles...

Embodiment 3

[0039] A kind of filling preparation, its preparation method comprises the following steps:

[0040] (1) Collect peripheral blood, centrifuge at 1200g / min for 8min, discard the platelet-poor plasma in the upper section and red blood cells in the lower section, store the platelet-rich plasma in the middle section at -60°C for 5 hours, take out the freeze-thaw, and then freeze and thaw the platelet-rich plasma Plasma was centrifuged at 2500rpm / min for 8min to remove membrane fragments at the bottom to obtain platelet factor-rich plasma for later use;

[0041] (2) Absorb autologous subcutaneous fat, let it stand, discard the upper layer of fat and wash it with sterile saline for 3 times to obtain fat particles, take half of the fat particles, add DMSO freezing solution twice the volume of fat particles to it, and remove the fat particles Carry out gradient cooling and freeze storage, then resuscitate the fat granules and wash away the DMSO freezing solution in the fat granules, t...

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Abstract

The invention discloses a filling preparation and a preparation method thereof. The preparation method comprises the following steps that peripheral blood is taken, centrifuging is conducted to obtain platelet-rich plasma, freezing is conducted, then the platelet-rich plasma is taken out and freeze-thawed, centrifuging continues to be conducted to remove membrane fragments at the bottom, and platelet-rich factor plasma is obtained for use; autologous subcutaneous fat is taken, fat granules are obtained and taken, DMSO refrigerating liquid is added in the granules for cryopreservation, then resuscitation is conducted, a sterile saline solution is added in the processed granules, a fat granule suspension is obtained, finally insulin is added in the fat granule suspension, the mixture is shaken evenly for use; the fat granules are centrifuged, upper oil is discarded, fat cells are obtained, and the fat cells are expanded and cultured to the P4 generation, and a fat stem cell resuspension solution is obtained for use; the platelet-rich factor plasma, the fat granule suspension and the fat stem cell resuspension solution are mixed evenly according to a certain volume ratio, and the filling preparation is obtained. According to the filling preparation, the problems that an existing filler material is low in survival rate, short in maintenance time after transplantation and poor in filling effect can effectively solved.

Description

technical field [0001] The invention relates to the technical field of filling preparations, in particular to a filling preparation and a preparation method thereof. Background technique [0002] With the growth of age, the face will gradually appear sunken, wrinkled, skin aging, etc., which is a big blow to people who are born with beauty. How to restore their face and skin to a youthful state has also become their pursuit. As a result, various facial filling materials and technologies have been born, among which autologous fat has become one of the ideal materials for tissue filling. [0003] Adipose tissue is an ideal source of autologous tissue cells, which can be obtained in large quantities through surgical liposuction with minimal trauma. In the past 20 years, autologous adipose tissue transplantation has been widely used in filling surgery and tissue reconstruction. These are not limited to trauma Defects on the back of the face or body, such as developmental disord...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/38A61L27/54
CPCA61L27/3604A61L27/3616A61L27/3687A61L27/3691A61L27/3834A61L27/54A61L2300/252A61L2300/412
Inventor 高雪华海泉
Owner CHENGDU QINGKE BIOTECH
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