A strain of Pichia monteritima that degrades polyethylene and produces alkanes

A polyethylene, yeast technology, applied in the direction of microbe-based methods, microbes, microbes, etc.

Active Publication Date: 2022-05-03
NORTHEAST FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, most of the research on the conversion of polyethylene into alkanes is based on physical and chemical methods. For example, Jia X et al. reported that under mild conditions, a series of catalytic cross-alkane metathesis methods were used to convert polyethylenes of different molecular weights and types into useful liquid fuels. and waxes, but there are few reports on the generation of alkanes through microbial degradation of polyethylene. Therefore, it is of great research value to seek bacterial strains that can degrade polyethylene and produce alkanes at the same time, which provides a basis for the biodegradation of polyethylene and the synthesis of mixed alkanes. new research direction

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  • A strain of Pichia monteritima that degrades polyethylene and produces alkanes
  • A strain of Pichia monteritima that degrades polyethylene and produces alkanes
  • A strain of Pichia monteritima that degrades polyethylene and produces alkanes

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specific Embodiment approach 1

[0015] Specific embodiment 1: Pichia pastoris MeyerozymaguilliermondiiZJC-1, which can degrade polyethylene in this embodiment, is preserved in the General Microbiology Center (CGMCC) of China Microbial Culture Collection Management Committee, and the preservation address is No. 1, Beichen Road, Chaoyang District, Beijing Court No. 3, the deposit date is December 17, 2018, and the deposit number is CGMCC No: 16956.

[0016] In this embodiment, Pichia guilliermondii ZJC-1 is obtained by screening from the intestinal fluid of the larvae of the mellonella mellonella, and is identified according to the following method.

[0017] Molecular biological identification of the screened strains: Inoculate Meyerozyma guilliermondii ZJC-1 in YPD liquid medium, culture in a constant temperature shaking incubator at 30°C at 120r / min for 16-24h, take 2mL of the bacterial liquid and centrifuge at 8000rpm to collect the bacterial cells , Genomic DNA was extracted according to the OMEGA HP Funga...

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Abstract

A strain of Pichia montonii that degrades polyethylene and produces alkanes simultaneously involves a strain of Pichia mongoliana. The invention provides a strain of Pichia mongolica, which provides a new bacterial source for the biodegradation of polyethylene and the production of alkanes. This season is Pichia pastoris Meyerozyma guilliermondii ZJC‑1, which is preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms. The preservation address is No. 3, No. 1 Beichen Road, Chaoyang District, Beijing, and the preservation date On December 17, 2018, the deposit number is CGMCC No: 16956. The Pichia pastoris Meyerozyma guilliermondii ZJC-1 of the present invention can degrade polyethylene and produce alkane substances simultaneously, providing a new bacterial source for the biodegradation of polyethylene and the biosynthesis of alkane. For the degradation of polyethylene, but also for the preparation of alkanes.

Description

technical field [0001] The present invention relates to a strain of Pichia mongolica. Background technique [0002] At present, most of the waste polyethylene plastics are mainly disposed of by incineration and landfilling. The landfilling of a large amount of polyethylene plastic waste has caused serious environmental hazards, such as destroying the physical and chemical properties of the soil, which in turn causes soil hardening, and incineration will cause serious environmental hazards. Generate CO, HCl, NOx, SO 2 A large number of toxic gases such as dioxins and dioxins will pollute the atmospheric environment. Both of these two processing methods will cause serious pollution to the environment. Therefore, a promising solution for waste polyethylene plastics is to convert them into valuable liquid fuels or Chemical materials. [0003] The main sources of alkanes at this stage are natural gas and petroleum. Although the composition of natural gas varies from place to p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12R1/84
CPCC12R2001/16C12N1/205C12R2001/84C12N1/165
Inventor 张杰盖玉杰王滨松刘长莉张国财池明眼王珊珊郝孔利董欣欣姬彦飞高超王天顺
Owner NORTHEAST FORESTRY UNIVERSITY
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