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Method for preparing tablets for karyotype analysis of chrysosplenium macrophyllum oliv.

A technique of chromosome preparation and golden waist, which is applied in the field of cytogenetics and cell biology, can solve the problems of cytoplasm residue, insufficient staining, insufficient stretching, etc., and achieve the effect of clear staining, good dispersion, and easy operation

Active Publication Date: 2019-09-20
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] According to the information, there is currently no method for preparing chromosomes of plants of the genus Chrysophylla, and the conventional chromosome preparation methods, that is, the pressing method and the wall-removing low-osmosis method, have many problems for the preparation of Chromosome chromosomes, such as chromosomes are difficult to disperse , Insufficient stretching, more cytoplasmic residues, insufficient staining, unstable film production results, etc.

Method used

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  • Method for preparing tablets for karyotype analysis of chrysosplenium macrophyllum oliv.
  • Method for preparing tablets for karyotype analysis of chrysosplenium macrophyllum oliv.
  • Method for preparing tablets for karyotype analysis of chrysosplenium macrophyllum oliv.

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Experimental program
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Effect test

Embodiment 1

[0054] The method for the preparation of the karyotype of Dayejinyao chromosomal type in embodiment 1

[0055] A method for preparing the karyotype of Dayejinyao chromosome, the specific experimental steps are as follows:

[0056] (1) Obtaining the root tip: at around 9:00 am on a sunny day, select a luxuriant plant in a potted plant, take about 3 cm of the root tip, and then clean the soil attached to the root surface with distilled water;

[0057] (2) Pretreatment: put the cleaned root tip into a vial filled with redistilled water, place it in a refrigerator at 4°C, and perform pretreatment for 2-3 hours, or use 0.02% colchicine and 2 mmol of 8- Hydroxyquinoline mixed solution is pretreated;

[0058] (3) Fix: absorb the water from the pretreated root tip, put it into a small bottle filled with fixative solution (ethanol: glacial acetic acid = 3:1), and store it fixedly, or transfer it to 75% ethanol solution after fixing for 24 hours For medium and long-term storage, store...

Embodiment 2

[0064] Example 2 Effects of Different Pretreatment Methods on Chromosome Karyotype of Dayejinyao

[0065] In order to explore the effects of different processing methods on the karyotype of the Chrysanthemum chinensis chromosome, and strive to find the most suitable pretreatment method for the karyotype preparation of the chromosomal karyotype of Chrysanthemum chinensis, the following experimental design was adopted in this implementation:

[0066] To compare the effects of the 4°C low-temperature treatment group and the colchicine and 8-hydroxyquinoline mixed solution treatment group on the karyotype of Yejinyao chromosome, three parallel experiments were set up for each group, and the specific steps were as follows:

[0067] 1. Experimental method

[0068] (1) Obtaining the root tip: at around 9:00 am on a sunny day, select a luxuriant plant in a potted plant, take about 3 cm of the root tip, and then clean the soil attached to the root surface with distilled water;

[0069...

Embodiment 3

[0078] Example 3 Effects of Different Enzyme Hydrolysis Parameters on the Chromosome Karyotype of Dayejinyao

[0079] In order to explore the influence of different enzymatic hydrolysis parameters on the karyotype of Dayejinyao chromosome, and strive to find the most suitable enzymatic hydrolysis parameter method for the preparation of Dayejinyao chromosome karyotype, the following experimental design was adopted in this implementation:

[0080] 1% mixed enzyme treatment group: 4% Cellulase R-10, 4% PECTOLYASE Y-23 were prepared into a mixed enzyme at a ratio of 1:1, and 1% of the above mixed enzyme was added during enzymatic hydrolysis;

[0081] 2% mixed enzyme treatment group: 4% Cellulase R-10, 4% PECTOLYASE Y-23 were prepared into a mixed enzyme at a ratio of 1:1, and 2% of the above mixed enzyme was added during enzymatic hydrolysis;

[0082] 2.5% mixed enzyme treatment group: 4% Cellulase R-10, 4% PECTOLYASE Y-23 were prepared into a mixed enzyme at a ratio of 1:1, and 2...

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Abstract

The invention relates to a method for preparing tablets for karyotype analysis of chrysosplenium macrophyllum oliv., which is a low evergreen herb and has an important medicinal development value because of containing flavonoids. The method for preparing tablets for karyotype analysis of chrysosplenium macrophyllum oliv. disclosed in the invention comprises the steps of: obtaining of root tips from a chrysosplenium macrophyllum oliv. fresh plant, preprocessing, fixing, pre- hypotension, enzymolysis wall removal, post-hypotension, smearing and dyeing, microscopic examination and observation. The invention provides a simple and applicable method for preparing tablets for karyotype analysis of chrysosplenium macrophyllum oliv., which provides help for subsequent scientific research of the chrysosplenium macrophyllum oliv., and lays a foundation for classification, development, application and research of chrysosplenium plants.

Description

technical field [0001] The invention belongs to the field of cytogenetics and cell biology, and in particular relates to a method for preparing chromosomes of a golden waist. Background technique [0002] Chrysosplenium macrophyllum Oliv. is a perennial herb of the genus Chrysosplenium in the family Saxifragaceae. Its leaves are alternate and have stalks. The leaves are nearly round to broadly ovate, with 11-13 round edges Teeth, ventral surface sparsely brown pilose, backside glabrous, petiole brown pilose. Flower stems sparsely brown villous. Basal leaves several, stalked, leathery, obovate, apex obtuse, entire or with inconspicuous wavy crenates, base cuneate, ventral surface sparsely brown pilose, back glabrous; cauline leaves usually 1 leaf blade, narrowly elliptic, usually with 13 crenates on the margin, glabrous on the back, sparsely brown pilose on the ventral surface and margin. Polychaste cymes; inflorescence branches sparsely brown pilose or nearly glabrous; br...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N1/28
CPCG01N1/30G01N1/2813G01N2001/302
Inventor 刘虹覃瑞吴智华吴士筠阮易柔黄文
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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