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A method for purifying chromium in water by using the interaction between Bacillus cereus and Li Shihe

A Bacillus cereus, water purification technology, applied in the field of water pollution treatment, can solve the problems of poor purification effect and low purification treatment efficiency, and achieve the effect of high reduction rate and short time

Active Publication Date: 2021-12-14
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] However, the current purification efficiency of Cr(Ⅵ) in water is low, and the purification effect is poor.

Method used

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  • A method for purifying chromium in water by using the interaction between Bacillus cereus and Li Shihe
  • A method for purifying chromium in water by using the interaction between Bacillus cereus and Li Shihe
  • A method for purifying chromium in water by using the interaction between Bacillus cereus and Li Shihe

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] The Bacillus cereus used in the experiment was purchased from the China Center for Type Culture Collection (CCTCC), numbered CCTCCAB2011085, and the strain was in the form of freeze-dried powder.

[0067] In the early stage of the test, Bacillus cereus was inoculated on the inclined tube LB medium, and after the strain was activated and recovered, the expansion culture was carried out (control shaker conditions: 30°C, 160r / min), and the bacteria in the Erlenmeyer flask grew turbid after 12 hours of culture. There is a bacterial film on the wall of the bottle, which is light milky white, and the sedimentation is stratified after standing. The OD600 of the bacteria is 0.8, and it is in the logarithmic growth phase. Inoculate to the third generation, after the metabolism of the strain is stable, the experiment is carried out.

[0068] Divide the enlarged Bacillus cereus into 10mL centrifuge tubes, place them in a high-speed refrigerated centrifuge and centrifuge at 8000r / m...

Embodiment 2

[0073] Select different forms of nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer, and add them to the pre-cultivated Lishihe at low, medium and high concentrations respectively. The pH of the solution was controlled at 7.3, and the dissolved oxygen DO of the solution was 4mg / L. The Lishihe after the pre-cultivation was moved into a plastic beaker with an initial concentration of Cr(VI) of 10mg / L and an initial concentration of bacterium of 0.1g / L. Bacillus cereus was expanded to the logarithmic growth phase in liquid LB medium, and the roots of Lishihe were protected from light, and repeated in three groups to test the influence of exogenous nutrients on the system, and 20 strains of Lishihe were placed in each repetition. Make sure that other conditions are the same during the experiment. The form and concentration of exogenous substances are shown in the table below:

[0074] Table 3 Form and concentration of exogenous nutrients

[0075]

[0076] ...

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Abstract

The invention provides a method for purifying chromium in water by using the interaction between Bacillus cereus and Lishihe, which belongs to the technical field of water pollution treatment. The method comprises the following steps: 1) Put Lishihe in culture solution for cultivation After 12 to 18 days, add hexavalent chromium with a final concentration of 8 to 12 mg / L in the culture solution to carry out stress cultivation for 8 to 12 days to obtain pre-cultured Lishihe; 2) place the pre-cultured Lishihe In the chromium-containing wastewater whose initial concentration of hexavalent chromium is 8-12mg / L, Bacillus cereus in the logarithmic growth phase is inserted into it to form a joint treatment system to treat the chromium-containing wastewater. By controlling the key physiological and ecological factors, the method gives full play to the synergistic effect of Bacillus cereus and Lishihe, and provides important theoretical reference and practical guidance for improving the efficiency of phytomicrobial remediation of Cr(VI) polluted water.

Description

technical field [0001] The invention belongs to the technical field of water pollution treatment, and in particular relates to a method for purifying chromium in a water body by utilizing the interaction between bacillus cereus and Lishihe. Background technique [0002] Chromium pollution is a relatively common heavy metal pollution in water, which has the characteristics of concealment, long-term and irreversibility. The wide application of chromium and its compounds has caused a large amount of chromium-containing wastewater to be discharged into water bodies, causing pollution to the environment. With the rapid development of industrial production, a large amount of chromium slag is produced in the production process, which has caused large-scale pollution to soil and water bodies. Seriously affect the ecological environment and human health. Therefore, how to effectively control chromium-containing wastewater and chromium slag to reduce pollution has become an urgent pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C02F3/32C12N1/20C02F101/22C12R1/085
CPCC02F3/34C02F3/32C12N1/20C02F2101/22C02F2305/06C02F2209/02C02F2209/06C02F2209/22
Inventor 林华满向甜刘立恒游少鸿刘杰林志毅
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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