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Prunus verecunda chloroplast genome and application thereof

A technology of chloroplast genome and Xiayingye, which is applied in the field of genetics

Active Publication Date: 2019-10-08
ZHEJIANG FORESTRY ACAD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the chloroplast genome of Xiaying cherry leaves is still blank

Method used

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  • Prunus verecunda chloroplast genome and application thereof
  • Prunus verecunda chloroplast genome and application thereof
  • Prunus verecunda chloroplast genome and application thereof

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Embodiment Construction

[0034] The present invention will be described in detail through specific examples below. These embodiments are provided for a more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.

[0035] "Includes" or "comprises" mentioned throughout the specification and claims is an open term, so it should be interpreted as "including but not limited to". The subsequent description in the specification is a preferred implementation mode for implementing the present invention, but the description is for the purpose of the general principles of the specification, and is not intended to limit the scope of the present invention. The scope of protection of the present invention should be defined by the appended claims.

[0036] Unless otherwise specified, various reagents and materials used in the present invention can be purchased from the market.

[0037] 1. Extract the total DNA of the sample

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Abstract

The invention discloses a prunus verecunda chloroplast genome for the first time, applies the prunus verecunda chloroplast genome to prunus verecunda system growth research, and analyzes the prunus verecunda growth status in prunus. According to the prunus verecunda chloroplast genome, molecular systematics and phylogeny genomics method are used for establishing phylogenetic relationship of subgenus cerasus species through the chloroplast genome, on the basis, the whole chloroplast genome is taken as a super DNA bar code of subgenus cerasus and used for identifying sakura varieties, technicalsupports for protecting of sakura germplasm resources and species identification are provided, and great significances on breeding typical subgenus cerasus plants, and the evaluation, protection and utilization of genetic diversity are brought.

Description

technical field [0001] The invention belongs to the field of gene technology, and in particular relates to the chloroplast genome of rosa japonica leaf and its application. Background technique [0002] With the development of society, human beings have increasingly urgent needs for the exploration of biodiversity, the rational use of biological resources and the rapid identification of species, and DNA barcoding technology has emerged as the times require. Originally, a DNA barcode referred to a standard DNA sequence used for species identification. The father of DNA barcoding, Hebert et al., successfully identified 200 species of Lepidoptera insects using a fragment of the mitochondrial cytochrome oxidase subunit 1 (cytochromeoxidase I, COI) gene of about 680 bp, and proposed DNA fragments for the first time as distinguishing species. Similar to the concept of DNA barcodes that distinguish goods by barcodes. Each species has a DNA sequence that distinguishes it from othe...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12Q1/6895G16B30/00
CPCC07K14/415C12Q1/6895G16B30/00
Inventor 沈鑫蒋冬月柳新红
Owner ZHEJIANG FORESTRY ACAD
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