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Fermentation method and application of human intestinal flora

A technology of intestinal flora and fermentation methods, applied in the fields of microorganisms, clinical, pharmaceuticals and health care products, can solve problems such as "unhealthy, affecting the effect of treatment"

Active Publication Date: 2021-04-02
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some apparently "healthy" donors may not have a "healthy" and balanced intestinal flora composition, and there may even be some potential opportunistic pathogens. If such a flora is transplanted to a patient, it may seriously affect the The effect of treatment

Method used

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  • Fermentation method and application of human intestinal flora
  • Fermentation method and application of human intestinal flora
  • Fermentation method and application of human intestinal flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A fermentation method of human intestinal flora, comprising the following steps:

[0034] (1) Screening of donors: select 3 boys aged 12-14 (2 12 years old, 1 13 years old), healthy, with no history of major diseases, no obvious gastrointestinal symptoms, and have not taken drugs for at least 6 months Any antibiotics, regular life.

[0035] (2) Sample collection and processing: collect fresh feces from each donor, quickly transfer to anaerobic workstation, weigh, add pre-reduced sterile saline at a ratio of 1:10 (w / v), vortex fully Spin and mix well; filter with double-layer gauze to collect feces suspension;

[0036] The pre-reduced sterile physiological saline is prepared from 0.9% sodium chloride solution, sterilized at 121° C. for 20 minutes, degassed at an anaerobic workstation and replaced with gas for more than 12 hours.

[0037] (3) Fermentation: Take 150 mL of fecal suspension from 3 donors, mix them evenly, dispense them into sterile serum bottles, 75 mL per...

Embodiment 2

[0044] A fermentation method of human intestinal flora, comprising the following steps:

[0045] (1) Screening of donors: select 3 boys aged 12-14 (2 12 years old, 1 13 years old), healthy, with no history of major diseases, no obvious gastrointestinal symptoms, and have not taken drugs for at least 6 months Any antibiotics, regular life.

[0046] (2) Sample collection and processing: collect fresh feces from each donor, quickly transfer to anaerobic workstation, weigh, add pre-reduced sterile saline at a ratio of 1:10 (w / v), vortex fully Spin and mix well; filter with double-layer gauze to collect feces suspension;

[0047] The pre-reduced sterile physiological saline is prepared from 0.9% sodium chloride solution, sterilized at 121° C. for 20 minutes, degassed at an anaerobic workstation and replaced with gas for more than 12 hours.

[0048] (3) Fermentation: Take 150 mL of fecal suspension from 3 donors, mix them evenly, dispense them into sterile serum bottles, 75 mL per...

Embodiment 3

[0052] A fermentation method of human intestinal flora, comprising the following steps:

[0053] (1) Screening of donors: A 12-year-old boy was selected, who was in good health, had no history of major diseases, had no obvious gastrointestinal symptoms, had not taken any antibiotics for at least 6 months, and had a regular life.

[0054] (2) Sample collection and processing: Collect the fresh feces of the donor, quickly transfer to the anaerobic workstation, weigh, add pre-reduced sterile saline at a ratio of 1:10 (w / v), and vortex fully. Evenly; filter with double gauze to collect the feces suspension;

[0055] The pre-reduced sterile physiological saline is prepared from 0.9% sodium chloride solution, sterilized at 121° C. for 20 minutes, degassed at an anaerobic workstation and replaced with gas for more than 12 hours.

[0056] (3) Fermentation: Take 225 mL of the donor's fecal suspension, divide it into 3 sterile serum bottles, each bottle is 75 mL, and seal it with a sil...

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Abstract

The invention provides a fermentation method of human intestinal flora and application. The method comprises the following steps of a, donor screening, wherein boys who are twelve to fourteen years old, are in good health, do not have the serious disease history or obvious gastrointestinal tract symptoms, do not take any antibiotics in at least six months and have a regular life are screened out;b, sample collection and treatment, wherein fresh excrement of the donors is collected, quickly transferred to an anaerobic working station and weighed, a pre-reduction sterile saline solution is added according to a proportion, and even mixing with sufficient vortex is conducted; then filtering is conducted, and an excrement suspension solution is collected; c, fermentation, wherein the excrementsuspension solution is transferred to a sterile serum bottle, sealed and placed in a culture box for standing fermentation. According to the fermentation method, the obtained pH-acidity fermentationproduct with multiple rich probiotics is a kind of optimized healthy human intestinal flora and can be applied to the excrement bacterium transplantation technology and development of medicines, health care products and the like with the human intestinal flora as a target point.

Description

technical field [0001] The invention relates to the fields of microorganisms, clinics, medicines and health care products, in particular to a fermentation method of human intestinal flora and its application. Background technique [0002] A large number and variety of microorganisms are colonized in the human intestinal tract. They are widely involved in physiological activities such as food digestion, metabolism and immunity of the human body, and are closely related to human health. A large number of studies in recent years have shown that the imbalance of intestinal flora is closely related to obesity, diabetes, inflammatory bowel disease, colorectal cancer, autism and other chronic diseases with high incidence in modern society. Play a role in prevention and treatment. [0003] Currently commonly used methods for regulating intestinal flora include taking probiotics or prebiotic substances, dietary intervention, and fecal microbiota transplantation. Among them, taking ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/741A61P29/00
CPCA61K35/741A61P29/00C12N1/20
Inventor 庞小燕赵立平付鑫超
Owner SHANGHAI JIAOTONG UNIV
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