Preparation method of paper-based photocathode biosensor for detecting microRNA

Abstract

The invention discloses a preparation method of a paper-based photocathode biosensor for detecting microRNA. A gold nano-particle layer is coated in a working area of a paper-based carbon working electrode; polyhedral cuprous oxide is electrodeposited and lots of silver sulfide quantum dots can be loaded, and a photoelectric sensitized structure is formed, so that the cathode photocurrent is increased substantially; and in the presence of the target microRNA, a double-strand-specific-nuclease-induced target cycle reaction and a cascaded stem-branch hybridization strand reaction are made to obtain a negatively charged dendritic DNA double-helix structure. After assembling of positively charged gold nanoparticles, a gold tree with the good glucose oxidase mimicking activity is formed, so that the glucose oxidation reaction is catalyzed, the dissolved oxygen of the electron acceptor is consumed, the cathode photocurrent signal is reduced, and sensitive detection of the microRNA is realized.

Description

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AI Technical Summary

Benefits of technology

This patented technology describes how small particles called metalloids (metal or semiconducting material that are tiny enough to attach themselves together). These metallurgies help create strong electrical connections with other materials like carbon nanotubes when they come into contact. By adding these metals onto certain surfaces during synthesis, this process allows for more precise control over particle size distribution and orientation within each clustered atom. It also enhances the efficiency at detecting DNA sequences through enzymatic reactions involving specific chemical groups attached thereto. Overall, this method provides technical means to make highly effective and efficient biochemistry tools such as probes used for diagnosing diseases.

Problems solved by technology

This patented technical problem addressed in this patents relates to developing efficient ways to measure small amounts (microscopes) of DNA or other nucleic acids without being affected by factors like pH changes caused by cells' activity.